Protocols for Micropropagation of Woody Trees and Fruits

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A. ONAY ET AL.
2.2.3. Rootstock Production
Pistacia vera, with reduced vigor, is commonly used rootstock worldwide. Most
pistachio orchards in Iran, Turkey, Syria and Tunisia are planted by using this
rootstock. As seed sources, in this study, the following open pollinated rootstocks
were used: Pistacia vera L., P. terebinthus L., and P. khinjuk Stocks. At the end of
14th day in vitro culture period, observations were taken from the total of 50
seedlings on: stem diameter (mm), stem height (cm), and length of the roots (cm).
Stem diameter. In vitro micrografting, stem diameter is very important. Onay et al.
(2003b) observed stem diameter differences between rootstocks. P. vera L. had the
biggest stem diameter which is important to accommodate the microscions while
P. terebinthus L. and P. khinjuk stocks showed a reduced stem diameter. As
expected, an effect was found between stem height records of the seedlings. In
adventitious roots.
In vitro seed germination type. This type was also tested for the best rootstock
production on RPM with or without 0.5 mg l –1 relation with the number of roots, P. vera L. had less roots with a couple of
BA. In this experiment, intact kernels,
half kernels with embryos were isolated and cultured on RPM. After 14 day culture
period, records on a total number of 40 explants were taken on stem diameter (mm),
stem height (cm), and length of roots (cm).
The cultured explants exhibited various responses according to with and without
BA treatments. In the presence of BA, the length of root was reduced, and the
isolated embryos gave shorter root length than the intact and half kernels. Regarding
stem diameter, important differences between rootstocks were observed (Onay et al.,
2003b). The intact kernels gave the biggest stem diameter among the explants tested.
Considering observations made, Pistacia vera L. is promising for in vitro micrografting.
Explant
1. Use mature dry nuts of Pistacia vera ‘Siirt’ to raise in vitro seedlings for
rootstocks.
2. Bring the seeds to the laboratory and remove outer pericarp and shells of
seeds before sterilization.
Surface sterilization of mature seed
1. Sterilize kernels, from which the outer pericarp and shells are removed by
immersion in a 20% (v/v) commercial bleach solution for 30 min on a
shaker at 150 rpm.
2. Gently wash with SDW at least 3 times for 5 min.
3. Shake with SDW at least 30 min on a shaker at 100 rpm.
4. Remove the seed coats and then cut the half of the cotyledons.
5. Incubate the cultures in Magenta GA 7 vessels (Chicago Corp.) containing
50 ml RPM supplemented with 1.0 mg l –1 BA in a growth room.