Protocols for Micropropagation of Woody Trees and Fruits

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APRICOT MICROPROPAGATION occurs frequently and needs to be controlled by immersing the shoot tips in concentrated BA solution. High percentages of apricot in vitro plantlet acclimatization can be achieved by using standard procedures. Micro propagated plants, on their own roots, are an alternative to grafted plants when the chosen rootstocks are apricot seedlings. A better root system and the elimination of problems related to the grafting point are characteristics of self-rooted plants obtained by micro propagation. Figure 9. Apricot shoots stored for 6 months without subculture at 3°C on a proliferation medium in darkness. 4. REFERENCES Cassells, A.C. (1991) Problems in tissue culture: culture contamination. In Debergh, P.C. & Zimmerman, R.H. (Eds) Micropropagation. Netherlands: Kluwer Academic Publishers, pp. 31–44. Driver, J.A. & Kuniyuki, A.H. (1984) In vitro propagation of Paradox walnut rootstock. HortScience 19, 507–509. Kataeva, N.V., Alexandrova, I.G., Butenko, R.G. & Dragavtceva, E.V. (1991) Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro. Plant Cell Tiss. Org. Cult. 27, 149–154. Koubouris, G. & Vasilakakis, M. (2006) Improvement of in vitro propagation of apricot cultivar ‘Bebecou’. Plant Cell Tiss. Org. Cult. 85, 173–180. Manganaris, G.A., Economou, A.S., Boubourakas, I.N. & Katis, N.I. (2003) Elimination of PPV and PNRSV thermotherapy and meristem-tip culture in nectarine. Plant Cell Rep. 22, 195–200. Marino, G., Bertazza, G., Magnanini, E. & Altan, A.D. (1993) Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of Apricot. Plant Cell Tiss. Org. Cult. 34, 235–244. Pérez-Tornero, O. & Burgos, L. (2000) Different media requirements for micropropagation of apricot cultivars. Plant Cell Tiss. Org. Cult. 63, 133–141. Pérez-Tornero, O., Burgos, L. & Egea, J. (1999a) Introduction and establishment of apricot in vitro through the regeneration of shoots from meristem tips. In Vitro Cell. Dev. Biol.-Plant 35, 249–253. Pérez-Tornero, O., Ortín-Párraga, F., Egea, J. & Burgos, L. (1999b) Medium-term storage of apricot shoot tips in vitro by minimal growth method. HortScience 34, 1277–1278. 277
278 O. PÉREZ-TORNERO AND L. BURGOS Pérez-Tornero, O., López, J.M., Egea, J. & Burgos, L. (2000) Effect of basal media and growth regulators on the in vitro propagation of the apricot (Prunus armeniaca) cv. ‘Canino’. J. Horticult. Sci. Biotechnol. 75, 283–286. Pérez-Tornero, O., Egea, J., Olmos, E. & Burgos, L. (2001) Control of hyperhydricity in micropropagated apricot cultivars. In Vitro Cell. Dev. Biol. 37, 250–254. Pérez-Tornero, O., López, J.M., García-Montiel, F. & Burgos, L. (2006) Field performance differences in three apricot cultivars propagated by tissue culture or by grafting. Acta Hort. 717, 255–258. Quoirin, M. & Lepoivre, P. (1977) Etude de milieux adaptes aux cultures in vitro de Prunus. Acta Hort. 78, 437–442. Reighard, G.L., Cain, D.W. & Newall, W.C., Jr. (1990) Rooting and survival potential of hardwood cuttings of 406 species, cultivars, and hybrids of Prunus. HortScience 25, 517–518. Snir, I. (1984) In vitro propagation of ‘Canino’ apricot. HortScience 19, 229–230. Vanderschaeghe, A.M. & Debergh, P.C. (1987) Technical aspects of the control of the relative humidity in tissue culture containers. Medelingen van de Faculteit Landbouwwetenschappen Rijksuniversitat Gent. 52, 1429–1437. Zimmerman, R.H. & Debergh, P.C. (1991) Micropropagation of temperate zone fruit and nut crops. In Zimmerman, R.H. & Debergh, P. (Eds), Micropropagation: Technology and application. Boston: Kluwer Academic, pp. 231–246.
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PROTOCOLS FOR MICROPROPAGATION OF W
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A C.I.P. Catalogue record for this
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vi TABLE OF CONTENTS 14. Root induc
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viii TABLE OF CONTENTS 43. Micropro
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x PREFACE on precise stepwise proto
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CHAPTER 1 TOTIPOTENCY AND THE CELL
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TOTIPOTENCY AND THE CELL CYCLE 5 a
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2.2. Plant Bioregulators and the Ce
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TOTIPOTENCY AND THE CELL CYCLE 9 in
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TOTIPOTENCY AND THE CELL CYCLE 11 F
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Bartel, D. (2004) MicroRNAs: genomi
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CHAPTER 2 MICROPROPAGATION VIA ORGA
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MICROPROPAGATION OF SLASH PINE Tabl
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MICROPROPAGATION OF SLASH PINE Amon
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2.6. Field Testing MICROPROPAGATION
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CHAPTER 3 MICROPROPAGATION OF COAST
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MICROPROPAGATION OF SEQUOIA SEMPERV
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CHAPTER 4 MICROPROPAGATION OF PINUS
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MICROPROPAGATION OF PINUS PINEA L.
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42 2.1. Organ Culture K. ISHII ET A
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44 K. ISHII ET AL. scent light, 70
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46 Chemicals (mg/l) K. ISHII ET AL.
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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116 D.T. NHUT ET AL. culture to be
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118 D. EWALD ability of the plant m
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120 D. EWALD Figure 1. Yew micropro
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122 D. EWALD Root development. Afte
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CHAPTER 12 MICROPROPAGATION OF LARI
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MICROPROPAGATION OF LARIX SPECIES V
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CHAPTER 13 PROPAGATION OF SELECTED
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PROPAGATION OF SELECTED PINUS GENOT
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CHAPTER 14 ROOT INDUCTION OF PINUS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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CHAPTER 15 MICROPROPAGATION OF BETU
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MICROPROPAGATION OF BETULA PENDULA
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CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
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DOUBLED-HAPLOID MICROPROPAGATION IN
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CHAPTER 17 IN VITRO PROPAGATION OF
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IN VITRO PROPAGATION OF FRAXINUS SP
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Axillary shoots (number) IN VITRO P
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CHAPTER 18 MICROPROPAGATION OF BLAC
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MICROPROPAGATION OF BLACK LOCUST 19
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2.5. Rooting and Acclimatization MI
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MICROPROPAGATION OF BLACK LOCUST 19
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202 V. RAJESWARI AND K. PALIWAL tha
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204 V. RAJESWARI AND K. PALIWAL Mak
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206 V. RAJESWARI AND K. PALIWAL 2.3
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208 V. RAJESWARI AND K. PALIWAL Fig
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210 2.8. Hardening V. RAJESWARI AND
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CHAPTER 20 MICROPROPAGATION OF SALI
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MICROPROPAGATION OF SALIX CAPREA L.
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CHAPTER 21 MICROPROPAGATION OF CEDR
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2.1. Establishment of Shoot Culture
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Page 248 and 249: CHAPTER 23 MICROGRAFTING IN GRAPEVI
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Page 252 and 253: MICROGRAFTING IN GRAPEVINE 253 Tabl
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Page 256 and 257: MICROGRAFTING IN GRAPEVINE 257 Feuc
Page 258 and 259: CHAPTER 24 MICROGRAFTING GRAPEVINE
Page 260 and 261: MICROGRAFTING GRAPEVINE FOR VIRUS I
Page 266 and 267: CHAPTER 25 APRICOT MICROPROPAGATION
Page 268 and 269: APRICOT MICROPROPAGATION Figure 1.
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Page 274 and 275: 2.4. Acclimatization APRICOT MICROP
Page 278 and 279: CHAPTER 26 IN VITRO CONSERVATION AN
Page 280 and 281: IN VITRO CONSERVATION AND MICROPROP
Page 288 and 289: CHAPTER 27 MICROGRAFTING OF PISTACH
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Page 298 and 299: CHAPTER 28 PROTOCOL FOR MICROPROPAG
Page 300 and 301: MICROPROPAGATION OF CASTANEA SATIVA
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Page 322 and 323: CHAPTER 30 IN VITRO MUTAGENESIS AND
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IN VITRO MUTAGENESIS AND MUTANT MUL
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336 R.I. IYER compounds (Iyer et al
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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374 L. TIAN AND S.I. SIBBALD younge
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376 L. TIAN AND S.I. SIBBALD Table
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378 L. TIAN AND S.I. SIBBALD 2.4. R
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CHAPTER 35 MICROPROPAGATION OF JUGL
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MICROPROPAGATION OF JUGLANS REGIA L
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CHAPTER 36 TISSUE CULTURE PROPAGATI
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PROPAGATION OF MONGOLIAN CHERRY AND
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410 M. PASQUAL AND E.A. FERREIRA 2.
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414 M. PASQUAL AND E.A. FERREIRA ch
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418 D.T. NHUT ET AL. its economical
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420 D.T. NHUT ET AL. Table 1. Shoot
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422 D.T. NHUT ET AL. Figure 3. Orga
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424 D.T. NHUT ET AL. mg l -1 NAA +
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426 D.T. NHUT ET AL. Nakasone, H.Y.
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428 C. LIU ET AL. C. cujete L. is c
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430 C. LIU ET AL. Table 1. Composit
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432 C. LIU ET AL. 6. Excise the sho
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434 Fresh weight per plantlet (mg)
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436 C. LIU ET AL. 4. REFERENCES Aga
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438 M. MISHRA ET AL. micropropagati
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440 M. MISHRA ET AL. each plate ino
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Section C
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446 M.G. OSTROLUCKÁ ET AL. from me
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448 M.G. OSTROLUCKÁ ET AL. regener
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450 M.G. OSTROLUCKÁ ET AL. Figure
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452 M.G. OSTROLUCKÁ ET AL. 2.6.3.
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454 counts counts M.G. OSTROLUCKÁ
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CHAPTER 42 PROTOCOL FOR MICROPROPAG
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AN medium (Anderson, 1980) MICROPRO
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MICROPROPAGATION OF VACCINIUM VITIS
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2.6. Flow Cytometry Analysis MICROP
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CHAPTER 43 MICROPROPAGATION OF BAMB
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BAMBOO MICROPROGATION BY AXILLARY S
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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