Protocols for Micropropagation of Woody Trees and Fruits

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-1 ).
S.J. MURCH ET AL.
Table 1. Media composition for micropropagation of breadfruit (Artocarpus altilis)
(all amounts given in mg l
Medium a
Components MSO b AS SAR LAR c
Major elements
NH 4 NO 3 1,650 1,650 1,650 1,650
KNO 3 1,900 1,900 1,900 1,900
CaCl 2 × 2H 2 O 440 440 440 440
MgSO 4 × 7H 2 O 370 370 370 370
Na 2 EDTA 37.3 37.3 37.3 37.3
KH 2 PO 4 170 170 170 170
FeSO 4 × 7H 2 O 27.8 27.8 27.8 27.8
Minor elements
H 3 BO 3 6.2 6.2 6.2 6.2
MnSO 4 × 4H 2 O 22.3 22.3 22.3 22.3
ZnSO 4 × 4H 2 O 8.6 8.6 8.6 8.6
KI 0.83 0.83 0.83 0.83
Na 2 MoO 4 × 2H 2 O 0.25 0.25 0.25 0.25
CoCl 2 × 6H 2 O 0.025 0.025 0.025 0.025
CuSO 4 × 5H 2 O 0.025 0.025 0.025 0.025
Organic constituents
Sucrose 30,000 30,000 30,000 30,000
Glycine 2 2 2 2
Myo-inositol 100 100 100 100
Nicotinic acid 1 1 1 1
Pyridoxine-HCl 1 1 1 1
Thiamine-HCl 10 10 10 10
Growth regulators
BA – 0.450 – –
Kinetin – 0.645 – –
IAA – – 0.175 0.875
Solidifying agent
Gelrite 2,200 2,200 2,200 –
AS, Artocarpus altilis Shoot (AS) medium; SAR, solid Artocarpus altilis rooting
medium; LAR, liquid Artocarpus altilis rooting.
a
pH of all media was adjusted to 5.75 before the addition of solidifying agent and
sterilization in the autoclave.
b –1
MSO medium used in the first shoot tip culture contain 1 ml l PPM.
c
LAR medium contains 5 times more IAA than SAR in the first step of mass
propagation in liquid system.