Protocols for Micropropagation of Woody Trees and Fruits

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424 D.T. NHUT ET AL. mg l –1 NAA + 30 g l –1 –1 –1 –1 NAA + 30 g l NAA + 30 g l –1 –1 NAA + 30 g l –1 mg l –1 IAA + 20 g l –1 Figure 5. Effects of various media on rhizogenesis. a) MS + 30 g l sucrose; b) MS + 0.5 sucrose; c) MS + 1.0 mg l sucrose; d) MS + 2.0 mg l sucrose; e) ½MS; f) ½MS + 1.0 mg l sucrose; g) MS + 1.0 sucrose; h) rooted plantlets in the greenhouse. –1
THIN CELL LAYER TECHNOLOGY AND YELLOW PASSION FRUIT 425 4. REFERENCES Appezzato-da-Glória, B., Veira, M.L.C. & Dornelas, M.C. (1999) Anatomical studies of in vitro organogenesis induced in leaf-derived explants of passion fruit. Pesq. Agro. Bras. 34, 2007–2011. Becerra, D.C., Forero, A.P. & Góngora, G.A. (2004) Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa. Plant Cell Tiss. Org. Cult. 79, 87–90. Biasi, L.A., Falco, M.C., Rodriguez, A.P.M. & Mendes, B.M.J. (2000) Organogenesis from internodal segments of yellow passion fruit. Sci. Agri. 57, 661–665. Coppens, E.G., Segura, S.D., Hodson de, J.E. & Góngora, G. (1997) Les fruits de la passion. In: Charrier, A., Jaquot, M., Hamon, S. & Nicolas, D. (Eds) L’amélioration des plants tropicales. CIRAD-OSTOM Coedition, Montpelier, France, pp. 291–312. Cronquist, A. (1981) An integrated system of classification of flowering plants. Columbia University Press. New York. Dornelas, M.C. & Vieira, M.L.C. (1993) Plant regeneration from protoplast culcures of Passflora edulis var. flavicarpa Deg., P. amethystina Mikan, and P. cincinnata Mast. Plant Cell Rep. 13, 103–106. Dornelas, M.C. & Vieira, M.L.C. (1994) Tissue culture studies on species of Passiflora. Plant Cell Tiss. Org. Cult. 36, 211–217. Drew, R.A. (1991) In vitro culture of adult and juvenile bud explants of Passiflora species. Plant Cell Tiss. Org. Cult. 26, 23–27. Drew, R.A. (1997) Micropropagation of Passiflora species (Passion fruit). In: Bajaj, Y.P.S. (Ed.) Hightech and micropropagation (V). Berlin, Springer-Verlag, pp. 135–149. D’Utra Vaz, F.B., Santos, A.V.P., Marders, G., Cocking, E.C., Davey, M.R. & Power, J.B. (1993) Plant regeneration from leaf mesophyll protoplasts of the tropical woody plant, passion fruit (Passiflora edulis var. flavicarpa Degner.): the importance of antibiotic cefotaxime in the culture medium. Plant Cell Rep. 12, 220–225. Faria, J.LC. & Segura, J. (1997a) Micropropagation of yellow passion fruit by auxillary bud proliferation. HortSci. 32, 1276–1277. Faria, J.L.C. & Segura, J. (1997b) In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis f. flavicarpa. In Vitro Cell. Dev. Biol.-Plant. 33, 209–212. Fouqué, A. (1972) Espècies frutières d’Amerique tropicale. Fruits. 27, 369–382. Gardner, D.E. (1989) Pathogenicity of Fusarium oxysporum f. sp. passiflorae to Banana Poka and other Passiflora spp. in Hawaii. Plant Disease. 73, 476–478. Guzzo, F., Ceoldo, S., Andretta, F. & Levi, M. (2004) In vitro culture from mature seeds of Passiflora species. Sci. Agric. 61, 108–113. Isutsa, D.K. (2004) Rapid micropropagation of passion fruit (Passiflora edulis Sims.) varieties. Sci. Hort. 99, 395–400. Kantharajah, A.S. & Dodd, W.A. (1990) In vitro micropropagation of Passiflora edulis (purple passion fruit). Ann. Bot. 65, 336–339. Kawata, K., Ushida, C., Kawai, F., Kanamori, M. & Kuriyama, A. (1995) Micropropagation of passion fruit from subcultured multiple shoot primordial. J. Plant Physiol. 147, 281–284. Killip, E.P. (1938) The American species of Passifloraceae. Field Museum of Natural History, Chicago. Mohamed, M.E., Hicks, R.G.T. & Blakesley, D. (1996) Shoot regeneration from mature endosperm of Passiflora foetida. Plant Cell Tiss. Org. Cult. 46, 161–164. Monteiro, A.C.B. de Almeida, Nakazawa, G.T., Mendes. B.M.J. & Rodriguez, A.P.M. (2000) In vitro regeneration of Passiflora suberosa from leaf discs. Sci. Agri. 57, 571–573. Moran Robles, M.J. (1978) Multiplication vegetative in vitro, des borgeons axilaires de Passiflora edulis var. flavicarpa Degemer et de P. molissima Bailey. Fruits. 33, 693–699. Moran Robles, M.J. (1979) Potential morphogenetique des entrenoeuds de Passiflora edulis var. flavicarpa Degemer et de P. molissima Bailey en culture in vitro. Turrialba. 29, 224–228. Murashige, T. & Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497.
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PROTOCOLS FOR MICROPROPAGATION OF W
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A C.I.P. Catalogue record for this
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vi TABLE OF CONTENTS 14. Root induc
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viii TABLE OF CONTENTS 43. Micropro
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x PREFACE on precise stepwise proto
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CHAPTER 1 TOTIPOTENCY AND THE CELL
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TOTIPOTENCY AND THE CELL CYCLE 5 a
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2.2. Plant Bioregulators and the Ce
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TOTIPOTENCY AND THE CELL CYCLE 9 in
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TOTIPOTENCY AND THE CELL CYCLE 11 F
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Bartel, D. (2004) MicroRNAs: genomi
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CHAPTER 2 MICROPROPAGATION VIA ORGA
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MICROPROPAGATION OF SLASH PINE Tabl
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MICROPROPAGATION OF SLASH PINE Amon
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2.6. Field Testing MICROPROPAGATION
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CHAPTER 3 MICROPROPAGATION OF COAST
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MICROPROPAGATION OF SEQUOIA SEMPERV
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CHAPTER 4 MICROPROPAGATION OF PINUS
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MICROPROPAGATION OF PINUS PINEA L.
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42 2.1. Organ Culture K. ISHII ET A
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44 K. ISHII ET AL. scent light, 70
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46 Chemicals (mg/l) K. ISHII ET AL.
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48 Table 3. Effects of plant growth
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50 K. ISHII ET AL. Gupta, P.K. & Du
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52 C. HARGREAVES AND M. MENZIES (Me
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54 C. HARGREAVES AND M. MENZIES Con
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56 2.1.3. Organogenesis from Field-
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58 C. HARGREAVES AND M. MENZIES Fig
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60 C. HARGREAVES AND M. MENZIES Fig
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62 C. HARGREAVES AND M. MENZIES For
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64 C. HARGREAVES AND M. MENZIES and
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CHAPTER 7 GENETIC FIDELITY ANALYSES
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PLANT GENETIC FIDELITY 69 Plant mat
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e added to samples, as this fluoroc
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11. Determine the mean channel numb
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3. In addition to testing various b
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GeneScan internal size standards: 4
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3500 3000 2500 2000 1500 1000 500 0
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PLANT GENETIC FIDELITY 81 microprop
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PLANT GENETIC FIDELITY 83 Steinkell
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86 2.1. Explant Preparation M.G. OS
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88 WPM (Lloyd & McCown, 1980) Macro
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90 M.G. OSTROLUCKÁ ET AL. on the m
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CHAPTER 9 MICROPROPAGATION OF MEDIT
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2.1. Explant Preparation MICROPROPA
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MICROPROPAGATION OF MEDITERRANEAN C
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108 D.T. NHUT ET AL. Larue (1953) a
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110 D.T. NHUT ET AL. HgCl2 added wi
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112 2.4. Establishment of Shoot Cul
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114 D.T. NHUT ET AL. Table 5. Effec
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116 D.T. NHUT ET AL. culture to be
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118 D. EWALD ability of the plant m
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120 D. EWALD Figure 1. Yew micropro
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122 D. EWALD Root development. Afte
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CHAPTER 12 MICROPROPAGATION OF LARI
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MICROPROPAGATION OF LARIX SPECIES V
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CHAPTER 13 PROPAGATION OF SELECTED
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PROPAGATION OF SELECTED PINUS GENOT
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CHAPTER 14 ROOT INDUCTION OF PINUS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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ROOT INDUCTION OF PINUS SYLVESTRIS
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CHAPTER 15 MICROPROPAGATION OF BETU
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MICROPROPAGATION OF BETULA PENDULA
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CHAPTER 16 PROTOCOL FOR DOUBLED-HAP
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DOUBLED-HAPLOID MICROPROPAGATION IN
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CHAPTER 17 IN VITRO PROPAGATION OF
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IN VITRO PROPAGATION OF FRAXINUS SP
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Axillary shoots (number) IN VITRO P
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CHAPTER 18 MICROPROPAGATION OF BLAC
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MICROPROPAGATION OF BLACK LOCUST 19
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2.5. Rooting and Acclimatization MI
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MICROPROPAGATION OF BLACK LOCUST 19
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202 V. RAJESWARI AND K. PALIWAL tha
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204 V. RAJESWARI AND K. PALIWAL Mak
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206 V. RAJESWARI AND K. PALIWAL 2.3
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208 V. RAJESWARI AND K. PALIWAL Fig
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210 2.8. Hardening V. RAJESWARI AND
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CHAPTER 20 MICROPROPAGATION OF SALI
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MICROPROPAGATION OF SALIX CAPREA L.
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CHAPTER 21 MICROPROPAGATION OF CEDR
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2.1. Establishment of Shoot Culture
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MICROPROPAGATION OF CEDRELA FISSILI
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238 programmes is somatic embryogen
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240 Figure 2. A) Induction of organ
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242 2.4.1. Rooting of Apical and Ba
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244 of donor individuals and/or by
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246 J. MALÀ ET AL. Karnosky, D.F.,
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CHAPTER 23 MICROGRAFTING IN GRAPEVI
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MICROGRAFTING IN GRAPEVINE 251 and
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MICROGRAFTING IN GRAPEVINE 253 Tabl
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2.4. Culture Conditions MICROGRAFTI
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MICROGRAFTING IN GRAPEVINE 257 Feuc
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CHAPTER 24 MICROGRAFTING GRAPEVINE
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MICROGRAFTING GRAPEVINE FOR VIRUS I
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CHAPTER 25 APRICOT MICROPROPAGATION
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APRICOT MICROPROPAGATION Figure 1.
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APRICOT MICROPROPAGATION Figure 2.
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APRICOT MICROPROPAGATION 2.2.2. Hyp
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2.4. Acclimatization APRICOT MICROP
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APRICOT MICROPROPAGATION occurs fre
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CHAPTER 26 IN VITRO CONSERVATION AN
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IN VITRO CONSERVATION AND MICROPROP
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CHAPTER 27 MICROGRAFTING OF PISTACH
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MICROGRAFTING OF PISTACHIO Constitu
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MICROGRAFTING OF PISTACHIO 2.2.2. C
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MICROGRAFTING OF PISTACHIO 6. Incub
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MICROGRAFTING OF PISTACHIO 9. The r
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CHAPTER 28 PROTOCOL FOR MICROPROPAG
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MICROPROPAGATION OF CASTANEA SATIVA
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CHAPTER 29 MICROPROPAGATION OF CASH
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MICROPROPAGATION OF CASHEW 2.2.1. E
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MICROPROPAGATION OF CASHEW axillary
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MICROPROPAGATION OF CASHEW Table 1.
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MICROPROPAGATION OF CASHEW shade an
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CHAPTER 30 IN VITRO MUTAGENESIS AND
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IN VITRO MUTAGENESIS AND MUTANT MUL
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336 R.I. IYER compounds (Iyer et al
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A 338 2.2. Culture Medium R.I. IYER
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340 R.I. IYER Figure 2. Formation o
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342 R.I. IYER Figure 3. Formation o
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344 R.I. IYER Rao, Y.S., Mathew, K.
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346 B.K. BISWAS AND S.C. GUPTA marg
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348 B.K. BISWAS AND S.C. GUPTA (iv)
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350 B.K. BISWAS AND S.C. GUPTA 2.4.
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352 B.K. BISWAS AND S.C. GUPTA Figu
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354 B.K. BISWAS AND S.C. GUPTA tree
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356 B.K. BISWAS AND S.C. GUPTA Figu
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358 B.K. BISWAS AND S.C. GUPTA Drew
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CHAPTER 33 MICROPROPAGATION PROTOCO
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MICROPROPAGATION FOR MICROSPORE EMB
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CHAPTER 44 IN VITRO CULTURE OF TREE
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IN VITRO CULTURE OF TREE PEONY 479
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500 M. MHATRE from various natural
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502 2.2. Disinfection of Plant Mate
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504 M. MHATRE soil in polythene bag
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506 M. MHATRE Figure 2. Pineapple,
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508 M. MHATRE Escalona, M., Lorenzo
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510 J.M. AL-KHAYRI Tunisia, Morocco
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512 J.M. AL-KHAYRI 2.1.2. Separatio
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514 2.2. Culture Medium J.M. AL-KHA
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516 J.M. AL-KHAYRI 5. Isolate callu
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518 J.M. AL-KHAYRI 3. Water the tra
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520 J.M. AL-KHAYRI expressed from c
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522 J.M. AL-KHAYRI alcohol and twic
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524 J.M. AL-KHAYRI potential. Simil
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526 J.M. AL-KHAYRI Barreveld, W.H.
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528 D.T. NHUT ET AL. arsenide chip
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530 D.T. NHUT ET AL. Figure 2. Plan
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532 D.T. NHUT ET AL. Figure 4. Plan
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534 D.T. NHUT ET AL. plantlets. The
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536 D.T. NHUT ET AL. Figure 11. Fre
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538 D.T. NHUT ET AL. Figure 13. Sub
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540 D.T. NHUT ET AL. The response o
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CHAPTER 48 IN VITRO MUTAGENESIS IN
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IN VITRO MUTAGENESIS IN BANANA 545
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3.3. Observations to be Recorded IN
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