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Table 2.
Lung adenoma incidence in male and female A/J mice exposed to acrylamide by
intraperitoneal injection (Bull et al., 1984a)
Dose level 1
Percent of animals with tumors
(mg/kg body weight)
males
females
0 13 8
1 50 35
3 38 53
10 59 79
30 93 93
1. The exposure duration was less than lifetime (8 weeks); the dose level listed was not adjusted to
reflect a less-than-lifetime exposure.
Robinson et al. (1986) exposed female SENCAR, BALB/c, A/J and ICR-Swiss mice (60
mice/strain/treatment group) to a single 50 mg/kg body weight dose of acrylamide by intraperitoneal
injection; 2 days later 40 of the 60 mice in each treatment group received 1.0 µg (SENCAR), 2.5 µg
(A/J and ICR-Swiss) or 5.0 µg (BALB/c) TPA in 0.2 ml acetone applied dermally 3 times/week for 20
weeks. The remaining 20 mice/strain/treatment group received acetone alone for the same treatment
schedule and duration. All animals were sacrificed at 40 weeks, and were only examined for the
number of skin papillomas and lung adenomas/animal. Acrylamide induced a significant increase in the
number of skin papillomas and lung adenomas per animal in SENCAR mice receiving TPA treatment.
The total number of animals bearing tumors was not listed. No significant increase in either tumor type
was noted in the other mouse strains tested; tumor data for the animals receiving acrylamide but not
TPA was not reported.
Male and female Fischer 344 rats (90/sex/treatment group) were exposed to acrylamide in drinking
water for 2 years (Johnson et al., 1986). Acrylamide water concentrations were adjusted to provide
dosages of 0, 0.01, 0.1, 0.5 or 2 mg/kg body weight/day. Interim sacrifices (10 animals/sex/treatment
group) were performed at 6, 12 and 18 months. A maximum tolerated dose (MTD) was achieved
based on decreased weight gain, increased mortality during the last 4 months of the study and the
appearance of several toxic effects (including peripheral nerve degeneration) in the 2 mg/kg/day group.
Increases in the incidences of a number of tumor types were observed in the 2.0 mg/kg/day exposure
group animals. An increased incidence of thyroid gland-follicular epithelium tumors was observed in
both males and females. In females, increased tumor incidences were noted in the mammary glands,
central nervous system, oral tissues, uterus and clitoral gland. An increased incidence of scrotal
mesothelioma was noted in males, in both the 2.0 and 0.5 mg/kg/day exposure group; additionally,
although not statistically significant, the incidence of scrotal mesothelioma in the 0.1 mg/kg/day group
was greater than either the control group or historical control incidences. Male rats in the 2.0
mg/kg/day exposure group also had a significant increase in adrenal pheochromocytomas, and an
increased incidence of central nervous system tumors when compared to historical controls but not
when compared to concurrent controls. Tumor incidence data is listed in Table 3.
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