studies

HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 389A
that mediates doxorubicin sensitivity through a process that
involves its acetylation and S574 phosphorylation. SIRT6 can
deacetylate FOXO3 preventing apoptosis, and TACE-resistant
tumors have higher SIRT6 levels. Manipulation of FOXO3 modifications
may thus prove useful in enhancing the chemotherapy
sensitivity of HCC.
Disclosures:
Steven A. Weinman - Consulting: Cardax, Inc.
The following authors have nothing to disclose: Josiah Cox, Zhuan Li, Anusha
Vittal, Maura O’Neil, Brian Bridges, Sean Kumer, Timothy Schmitt
353
Long non-coding RNA integrator complex subunit 6
pseudogene 1 (INTS6P1), a tumor suppressor and prognostic
factor in hepatocellular carcinoma, induces apoptosis
via intrinsic mitochondrial pathway
Minqiang Lu 1 , Ka Yin LUI 1 , Haoran Peng 1,2 , Rongdang Fu 1 , Huan
Chen 1 , Chunhui Qiu 1 ; 1 Hepatic Surgery, The Third Affiliated Hospital
of Sun Yat-sen University, Guangzhou, China; 2 Division of
Gastroenterology and Hepatology, The Johns Hopkins Hospital,
Baltimore, MD
Purpose: Long non-coding RNAs (lncRNAs) have been proved
its close association with many diseases especially with tumor.
We aimed to determine whether the lncRNA integrator complex
subunit 6 pseudogene 1 (INTS6P1) could be as a tumor
suppressor and its mechanism in hepatocellular carcinoma
(HCC). Materials and methods: Firstly, the expression level of
INTS6P1 mRNA were measured in a cohort of 60 HCC tissues
and adjacent normal liver tissues by using the quantitative
real time-polymerase chain reaction (qRT-PCR); Secondly, the
functional studies of INTS6P1, include growth curves, migration
assays and cell death, were detected in HCC cell lines.
Finally, the mechanism experiment was investigated for tumor
suppressive roles of INTS6P1. Results: We found that lncRNA
INTS6P1 was remarkably down-regulated in HCC (71.4%),
and its expression was significantly correlated with pathology
grade (p