studies

HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 1011A
MX1 N-terminal domain to specifically identify its effects on
HBV and HCV replication is warranted.
Disclosures:
Dahlene Fusco - Grant/Research Support: Gilead
Raymond T. Chung - Grant/Research Support: Gilead, Mass Biologics, Abbvie,
Merck, BMS
The following authors have nothing to disclose: Wenyu Lin, Dachuan Cai, Jian
Hong, Xiao Liu, Hong Zhao, Chuanlong Zhu, Esperance A. Schaefer, Cynthia
Brisac, Sae Hwan Lee, Anna Lidofsky
Disclosures:
Kazuaki Chayama - Consulting: AbbVie; Grant/Research Support: Ajinomoto,
Astellas, Torii, Tsumura, Aska, Bayer, Zeria, Daiichi Sankyo, Dainippon Sumitomo,
Eisai, Eli Lily, Janssen, Kowa, Mitsubishi Tanabe, MSD, Nippon Kayaku,
Nippon Shinyaku, Otsuka, Roche, Takeda, Toray; Speaking and Teaching:
Ajinomoto, AbbVie, Abott, Astellas, AstraZeneca, Aska, Bayer, BMS, Chugai,
Daiichi Sankyo, Dainippon Sumitomo, Eisai, J & J, Jimro, Miyarisan, MSD, Nihon
Kayaku, Olympus
The following authors have nothing to disclose: Masataka Tsuge, Nobuhiko
Hiraga, Hiromi Abe, Daiki Miki, Michio Imamura, Hidenori Ochi, C. Nelson
Hayes
1645
Difference of intracellular responses by HBV genotype A
and C infection in humanized mouse model
Masataka Tsuge 1 , Nobuhiko Hiraga 1 , Hiromi Abe 1 , Daiki Miki 2 ,
Michio Imamura 1 , Hidenori Ochi 1 , C. Nelson Hayes 1 , Kazuaki
Chayama 1 ; 1 Department of Gastroenterology and Metabolism,
Hiroshima university, Hiroshima, Japan; 2 Institute of Physical and
Chemical Research (RIKEN), Hiroshima, Japan
Background & Aims: It is well known that the characteristics of
chronic hepatitis B, including the clinical course and responsiveness
to anti-viral treatments, differ among hepatitis B virus
(HBV) genotypes. However, the cause of these differences has
not been clarified. We have previously established a chronic
HBV infection model using human hepatocyte transplanted
uPA-SCID mice lacking human immune cells. We previously
reported the effects of HBV infection in human hepatocytes
using cDNA microarray. In the present study, we analyzed
mRNA expression profiles using next generation sequencing in
this mouse model and compared the responses between HBV
genotypes A and C. Methods: Fifteen chimeric mice were prepared
and divided into the following three groups: uninfected
control mice, HBV genotype A infection, and HBV genotype C
infection. Ten mice were inoculated with HBV genotype A or C,
and human hepatocytes in the mouse livers were collected after
mouse serum HBV DNA titers had reached a plateau of around
8 Log copies/ml. To analyze changes in gene expression in
human hepatocytes, we performed next generation sequencing
and compared gene expression profiles. Results: HBV genotype
A and C infection resulted in significant changes in mRNA
expression levels in HBV-infected hepatocytes in 780 and 208
genes, respectively (P