studies

HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 527A
sion: IgG4-RD of the biliary tree and pancreas can be accurately
distinguished from PSC or pancreatobiliary malignancies
by ΔC T
based on an affordable qPCR test. ΔC T
can also be
used as a marker for treatment response and disease activity
in IAC and AIP.
Figure 1.
Disclosures:
Ulrich Beuers - Consulting: Intercept via University of Amsterdam, Novartis via
University of Amsterdam; Grant/Research Support: Falk, Zambon; Speaking and
Teaching: Falk Foundation, Gilead, Roche, Shire
The following authors have nothing to disclose: Lowiek M. Hubers, Marieke E.
Doorenspleet, Emma L. Culver, Lucas Maillette de Buy Wenniger, Paul L. Klarenbeek,
Roger W. Chapman, Stan F. van de Graaf, Joanne Verheij, Thomas van
Gulik, Frank Baas, Eleanor Barnes, Niek de Vries
638
Emperipolesis mediated by CD8+ T cells correlated with
biliary epithelia cell injury in primary biliary cirrhosis
Suxian Zhao, Rong-qi Wang, Yuguo Zhang, Huijuan Du, Yuemin
Nan; Third Hospital of Hebei Medical University, Shijiazhuang,
China
Introduction: Primary biliary cirrhosis (PBC) is thought to be an
autoimmune disease characterized by chronic destruction of
bile ducts. A major unanswered question regarding the pathogenesis
of PBC is the precise mechanisms of small duct injury
and destruction. Emperipolesis is one of cell-in-cell structures
that have been observed in chronic viral hepatitis. However,
it remains unknown whether emperipolesis is involved in biliary
epithelia cell injury and it diagnostic value in PBC. Aims:
To clarify the pathogenesis and histological characteristics of
emperipolesis in the liver injury of PBC. Methods:Sixty PBC
patients, diagnosed by liver biopsy, were divided into two
groups, early PBC (stages I and IIn=40) and late PBC (stages III
and IV, n=20). The degrees of hepatic inflammation and fibrosis
were assessed with Scheuer Scoring System. Emperipolesis
was observed in liver sections stained with hematoxylin-eosin.
The expression of CK19 (cholangiocyte), CD8 (T cell), CD20 (B
cell), CD56 (NK cell), CD68 (macrophage cell) and MPO (neutrophil)
was evaluated by mmunofluorescence double labeling
combined with confocal microscopic observation. The apopotosis
of BECs was detected by TUNEL assay under laser confocal
microscope. Results: Emperipolesis was observed in 73.3%
of patients with PBC, and BECs were predominantly host cells.
In PBC patients with emperipolesis, the number of emperipolesis
structures correlated with lymphoid follicles (r=0.4394,
P