studies

HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 553A
Fig 1
results demonstrated that TSG-6 promoted the autophagical
function in hepatocytes, contributing to the liver regeneration.
Disclosures:
The following authors have nothing to disclose: Sihyung Wang, Jeongeun Hyun,
Jieun Kim, Youngmi Jung
Disclosures:
The following authors have nothing to disclose: Ritu Khosla, Archana Rastogi,
Shyam Singh, Madavan Vasudevan, Viniyendra Pamecha, Gayatri Ramakrishna,
Shiv K. Sarin, Nirupma Trehanpati
696
TSG-6 enhances autophagy in chronically damaged
liver
Sihyung Wang, Jeongeun Hyun, Jieun Kim, Youngmi Jung; Pusan
National University, Pusan, Korea (the Republic of)
Tumor necrosis factor-inducible gene 6 protein (TSG-6), one
of cytokines secreted from mesenchymal stem/stromal cells, is
known to act as an anti-inflammatory factor and reduce several
pathological conditions. Recently, TSG-6 has been shown to
protect liver from injury; however, the mechanism underlying the
effect is poorly understood. Autophagy is the catabolic process
that targets cell constituents to the lysosomes for degradation
and known to be dysregulated in several diseases, including
liver diseases. Emerging evidence suggests that the autopagic
functions protect hepatocytes from damages. Hence, we
hypothesize that TSG-6 promotes the autophagical clearance
systems in hepatocytes and protects liver from chronic injury.
To prove this hypothesis, mice were fed with Methionine choline-deficient
ethionine (MCDE) for 4 weeks, followed by being
injected intraperitonially with TSG-6 (50ng) (TSG-6 group) or
saline (MCDE+vehicle group) with MCDE supplemented diets
for additional 2 weeks. The histomorphological liver injury and
increased level of liver enzymes were shown in MCDE-treated
mice with or without saline, whereas those observations were
markedly ameliorated in TSG-6-treated mice with MCDE diet
(AST; control: 31.32±1.74, MCDE: 198.42±7.09, MCDE+vehicle:
184.35±26.01, MCDE+TSG-6: 140.91±31.33/ ALT;
control: 33.95±3.09, MCDE: 151.34±40, MCDE+vehicle:
156.14±32.34, MCDE+TSG-6:111.91±10.39). RNA analysis
showed the up-regulation of autophagy-relate genes, atg3 and
atg7 (2.38±0.37 fold increase for atg3, 1.8±0.17 fold increase
for atg7; p