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2376 J. Med. Plants Res.
Table 4. Recovery rate test.
Test No.
Flavonoids sample
(ml)
Table 2. Stability test.
Time (h) Absorbance
0 0.165
1 0.160
2 0.163
3 0.166
4 0.164
5 0.166
6 0.165
7 0.164
8 0.166
9 0.168
Table 3. Reappearance test.
Time (h) Absorbance (%) Extraction rate of flavonoids
1 0.163 2.48
2 0.166 2.53
3 0.167 2.54
4 0.161 2.45
5 0.159 2.42
6 0.163 2.48
Content of flavonoids
(mg)
Rutin standard sample
added (mg)
Total flavonoids
(mg)
Recovery
rate (%)
1 10 13.31 10.0 23.02 98.7
2 10 13.31 15.0 27.94 98.6
3 10 13.31 20.0 33.45 100.4
4 10 13.31 25.0 38.04 99.2
5 10 13.31 30.0 42.86 98.9
6 10 13.31 35.0 47.89 99.1
extraction. The RSD was 1.85% (RSD < 2.0%).
Recovery rate test
Table 4 shows that the RSD of the recovery rate test
was 0.66% (RSD < 2.0%).
Single factor experiments of hot reflux extractions
with ethanol
Influence of the alcoholic concentration on the
extraction
Figure 2 shows that the total flavonoids content increased
with increased alcoholic concentration. At alcohol
concentrations beyond 60%, no significant increase in
flavonoids content occurred. Hence, the alcoholic
concentrations (60, 70 and 80%) was then chosen for the
orthogonal experiments.
Influence of the solid-liquid ratio on the extraction
Figure 3 shows that at solid-liquid ratios beyond 1:30, the
total flavonoids content decreased. Hence, the solid-liquid
ratios of (1:20, 1:30 and 1:40) were chosen for the
orthogonal experiments.
Influence of the number of extraction on the
extraction
Figure 4 shows that when the number of extraction is