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(C-5), 41.3(C-3), 37.9 (C-1), 37.8 (C-10), 35.9 (C-
6),33.2 (C-4), 32.5 (C-2), 23.2 (C-18), 21.3 (C-19),
18.9 (C-20)。IR (KBr, cm -1 ): 3428, 2990,2915,
2820, 1645, 1590, 1505, 1430, 1355, 1323, 1278,
1188, 875. This spectral data of 1 H NMR and 13 C
NMR are in agreement with that of nimbidiol in the
literature (Majumder et al., 1987; Masahiro et al.,
2010).
Data of compound I molecular formula
C30H40O4. mp 217 to 219°C. EI- MS m/z
487[M+Na] + , 465[M+H] + 。IR (KBrυmax / cm -1 ):
3350, 2940, 1723, 1650, 1590, 1520, 1435,
1375,1300,1245,1220, 1205, 1185, 155, 1145,
1095, 1085, 990, 870, 860, 850。 1 H-NMR
(CDCl3): δ 7.03 (1H, d, J=9.2Hz, H-6), 6.98 (1H, s,
Figure 2. The HPLC chromatogram of compound II. Column: Waters symmetry C18 column
(150×4.6 mm); column temperature: 25°C; mobile phase: methanol: 0.1%phosphoric acid (88:12),
flow rate: 0.8 ml/ min; detection wavelength: 254 nm.
H-1), 6.36 (1H, d, J=7.2Hz, H-7), 6.53 (1H, brs,
disappearing after the exchange of D2O, -OH),
3.55 (3H, s, H-31), 2.22 (3H, s, H-23), 1.45 (3H, s,
H-25),1.26 (3H, s, H-26), 1.18 (3H, s, H-30), 1.08
(3H, s, H-28), 0.52 (3H, s, H-27)。 13 C-NMR:δ
119.8 (C-1), 178.6 (C-2), 146.2 (C-3), 117.3 (C-4),
127.6 (C-5), 133. 2(C-6),118.3 (C-7) 160.3 (C-8),
43.2 (C-9) 164.9 (C-10) 33.8 C-11) 29.9 (C-12)
39.6 (C-13), 45.3 (C-14), 28.9 (C-15), 36.6 (C-16),
30.8 (C-17), 44.5(C-18), 31.1 (C-19),40.6 (C-
20), 30.1 (C-21), 35.0 (C-22), 10.5 (C-23), 38.5
(C-25), 21. 8(C-26), 18.5 (C-27), 31.8(C-28),
178.9 (C-29), 32.9 (C-30), 51.8 (C-31). This
spectral data of 1 H NMR and 13 C NMR are in
agreement with that of pristimerin in the literature
(Jiang et al., 1996).
CONCLUSION
Xie et al. 2523
HSCCC was successfully used for the preparative
isolation of terpenoids. In combination with a
suitable extraction and cleanup procedure prior to
HSCCC separation, 2.5 mg nimbidiol and 3.0 mg
pristimerin were obtained from 250 mg of the
crude sample on a preparative scale. Meanwhile,
the isolation of nimbidiol and pristimerin for the
first time from C. aculeatus Merr proved HSCCC
to be a useful tool for the exploring of the
chemical component of traditional medicinal
herbs.