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2408 J. Med. Plants Res. Conclusion Table 2. The scavenging ratio on ·OH of extracts of 18 Chinese medicinal herbs (n = 5). Chinese medicine herb Scavenging rate (%) RSD (%) R. chinensis Mill. 97.7 0.21 T. chebula Retz. 96.8 0.10 F. suspensa (Thunb.) Vahl. 92.5 0.22 L. japonica Thunb. 91.5 0.59 R. chinensis Jacq. 89.5 1.6 R. glutinosa Libosch. 87.5 1.0 L. barbarum L. 86.8 1.1 S. baicalensis Georgi 86.7 1.7 A. sinensis (Oliv.) Diels 84.4 0.68 C. indicum L. 83.4 3.0 G. uralensis Fisch. 79.4 0.65 G. biloba L. 78.4 1.4 A. mongholicus Bge. 75.7 0.78 P. multiflorum Thunb. 74.2 1.4 S. chinenesis(Turcz.)Baill. 62.8 4.5 R. cordifolia L. 58.2 4.6 L. chiachristinae Hance 39.1 5.2 C. sativa L. 25.6 3.9 A new flow-injection chemiluminescence method for determination of scavenging capacity of Chinese medicinal herbs to hydroxyl radicals based on the inhibition effect of herb water extract on the CL intensity in methyleme blue-Fe 2+ -H2O2 system was established. The scavenging rates of the herbs could be measured even with a simple setup. It was believed that the reaction of methylene blue with hydroxyl radical was responsible for the CL emission in the CL system, while the degree of the reduction of the CL intensity resulted from the scavenging action of herb water extracts on hydroxyl radical had a stoichiometric relationship with herb concentration in the solution. The proposed method is not only simple and convenient, but also stable and user-friendly. It had been applied to the determination of hydroxyl radical scavenging capacity of 18 Chinese medicinal herbs with satisfactory results. ACKNOWLEGEMENTS We gratefully acknowledge financial support from the Guangxi Science Foundation of China (No. 2010GXNSFA013001). REFERENCES Cai YZ, Luo Q, Sun M, Corke H (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci., 74: 2157-2184. Chen HL, Gu YF, Wang YL (2006). Advance on the investigation of chemical composition and antioxidant activity of Chinese herbal medicine. Chin. J. Tradit. Med. Sci. Technol., 13: 63-64. Chen J, Li CF, Song GW (2008). Determination of elimination activity in vitro of bilirubin for the hydroxyl radical by UV-Vis spectrophotometry. J. Hebei Univ. (Nat. Sci. Ed.), 30(2): 199-201. Cheng FC, Jen JF, Tsai TH (2002). Hydroxyl radical in living systems and its separation methods. J. Chromatogr. B, 781: 481-496. Espinoza M, Olea AC, Speisky H, Rodri GJ (2009). Determination of reactions between free radicals and selected Chilean wines and transition metals by ESR and UV-vis technique. Spectrochim. Acta. 71: 1638-1643. Guo DJ, Cheng HL, Chan SW, Yu PHF (2008). Antioxidative activities and the total phenolic contents of tonic Chinese medicinal herbs. Inflammopharmacology, 16: 201-207. Ivan S, Milos M, Dusko B , Snezana DS, David RJ (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. Carbohydr. Res., 344: 80-84. Jiang SH, Li HQ,Ma HL, Liao L, Wang ZB,Fu X, Wang CL (2011). Antioxidant activities of selected Chinese medicinal and edible plants. Int. J. Food Sci. Nutr., 62: 441-444. Julio AZ, Kenneth FR (2009). Fenton’s oxidation of pentachlorophenol. Water Res., 43: 1831-1840. Kati H, Merike V, Tõnu P, Mihkel K (2009). Analysis of the stable free radical scavenging capability of artificial polyphenol mixtures and plant extracts by capillary electrophoresis and liquid chromatography–diode array detection–tandem mass spectrometry. J. Chromatogr. A, 1216: 2417-2423. Knight JA (1995). Diseases related to oxygen-derived free radicals. Ann. Clin. Lab. Sci., 25: 111-121. Kricka LJ (2003). Clinical applications of chemiluminescence. Anal. Chim. Acta, 500: 279-286. Lee YC, Chuah AM, Yamaguchi T, Takamura H, Matoba T (2008). Antioxidant activity of traditional Chinese medicinal herbs. Food Sci. Technol. Res., 14: 205-210. Liu F, Ng TB (2000). Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci., 66: 725-735. Long SJ, Shi JQ, Xie YF (2006). Detection of hydroxym radicals by flow injection phenanthroline chemiluminescence system. Chin. J. Anal. Lab., 25: 35-38.
Pan YM, Liang Y, Wang HS, Liang M (2004). Antioxidant activity of several Chinese medicine herbs. Food Chem., 88: 347-350. Pezo D, Salafranca J, Nerin CJ (2008). Determination of the antioxidant capacity of active food packagings by in situ gas-phase hydroxyl radical generation and high performance liquid chromatographyfluorescence detection. J. Chromatogr. A, 1178: 126-133. Qu P, Li GL, Xu MT (2004). A novel catalysis kinetics fluorimetry system for the determination of eliminating ratio of Chinese traditional medicine for •OH. Spectro. Spect. Anal., 24: 1407-1409. Shen GX, Zhang H, Kakii K (2007). Research progress of fenton reaction. Sci. Technol. Inform., 43: 24-25. Sheng L, WANG HX, Han XQ (2008). Determination of ascorbic acid by fluorescence quenching method with methylene blue. Chin. J. Anal. Lab., 27: 67-69. Zhuo et al. 2409 Wong CC, Li HB, Cheng KW, Chen F (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem., 97: 705–711. Yang JH, Li B, Ji BP (2006). Study on antioxidant activities of common Chinese edible plants and medicinal plants. Food Sci., 27: 87-90. Yang MH, Liu MH, He LX, Cao QE (2006). Determination of hydroxyl free radical in Fenton reaction by decolouring spectrophotometry and its analytical applications Chin. J. Anal. Lab., 25: 77-80. Zhang AM, Li LW, Hao GQ, Liu YG (2005). Spectrophotometric determination of antioxidant activity of Chinese traditional medicine with methylene blue-Mn 2+ -H2O2 system. Chin. J. Instrum. Anal., 24: 83-85.
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Journal of Medicinal Plants Researc
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Editors Prof. Akah Peter Achunike E
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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Table of Contents: Volume 6 Number
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Figure 1. Chuanxiong Rhizoma (http:
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Table 1. Identification of main pea
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Table 3. Pharmacokinetic parameters
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active component study, many invest
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and 1185 kg ha -1 in the first site
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Table 1. Recommendation for use fer
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Sadanandan AK, Hamza S (1996c). Stu
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and it has been traced to South Ame
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Table 3. Results of phytochemical a
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(a) (b) (c) Figure 1. (a) Normal ce
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emedies in the south of Brazil. J.
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known fatty acids and terpenoids we
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Table 1. Renal function tests of ra
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Al-Radahe et al. 2271 Figure 3.nnnn
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namely disruption of the vascular e
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Zayachkivska OS, Konturek SJ, Drozd
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β-carotene were purchased from Sig
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Figure 1. Effect of ethanol concent
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Table 3. Climatic and soil factors
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content of polysaccharides. Phospha
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Serge et al. 2285 Table 1. Relative
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Percentage inhibition % of inhibiti
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Table 1. Result of phytochemical sc
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Table 3. Result of thin layer chrom
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Table 2. MIC of a compound 1 from c
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Zirihi et al. 2301 Figure 2. Termin
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Figure 5. T. catappa Linn. (Combret
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Figure 8. A. fumigatus: Aspect of c
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Figure 12. Sensitivity of A. fumiga
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Noormi et al. 2311 Table 1. Callus
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Table 2. Contd. NoC=No callus. 4.0
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Figure 2. Contd. at 2.0 mg/L yellow
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Table 1. Biochemical parameters in
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Figure 3. Bcl-xL reactions in inter
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ACKNOWLEDGMENTS The authors would l
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Figure 1. The first page of the boo
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Figure 3. The most widely included
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Figure 4. Lithographic print “St.
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Table 1. List of plants included in
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Table 1. Contd. Nedelcheva 2333 Cin
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Table 1. Contd. Nedelcheva 2335 Rhe
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14 7 1 15 1 35 17 16 Imported plant
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Academy of Science Publishing House
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et al., 1996; van Wyk and Gericke,
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DPPH inhibition (%) DPPH % inhibiti
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% Mortality Concentration (µg/ml)
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information on plants used for the
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Table 1. Levels of independent vari
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Arbutin (mg/g) Co-solvent Figure 1.
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Arbutin (mg/g) Co-solvent Figure 3.
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Arbutin (mg/g) Extraction pressure
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2476 J. Med. Plants Res. Figure 1.
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2482 J. Med. Plants Res. Table 2. C
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2484 J. Med. Plants Res. Table 3. C
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2486 J. Med. Plants Res. components
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2490 J. Med. Plants Res. Inhibition
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2494 J. Med. Plants Res. can increa
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2500 J. Med. Plants Res. Flowering
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2524 J. Med. Plants Res. ACKNOWLEDG
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UPCOMING CONFERENCES 15th European
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