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Table 1. Comparison of survival times of S180 tumor-bearing mice among the 5 treatment groups. Group Death/total Mediated life span(d) Survival rate (%) NS 10/10 15.80±2.94 0 CTX 10/10 21.30±2.50* 0 LFP91-3I 8/10 23.00±2.62* 20 LFP91-3II 6/10 28.50±4.60* 40 LFP91-3III 5/10 33.60±3.64* 50 p95%, was determined by trypan blue staining. The cell concentration was adjusted to a final concentration of 1×10 7 cells per ml. 100 μl cell suspension was added into 96-well plate per well. After pre-incubating S180 tumor cells for 8 h at 37°C in 5% CO2 incubator, the control group contains normal saline 100 μl. 100 μl LFP91-3 with a final concentration (5 μg/ml) was added as the experimental group and incubated with the cells for 24, 48 and 72 h. The cells were collected and the cell concentration was adjusted to 5 × 10 5 /ml. The cells were washed twice with PBS, centrifuged at 1000 rpm for 5 min and the supernatants were aspirated. The cells were suspended with 500 μl 1× Binding Buffer, followed by adding 5 μl Annexin V-FITC, 5 μl PI (BioVision Annexin V-FITC Apoptosis Detection Kit), keep in dark for 5min at room temperature, and then detected through flow cytometry. Statistical analyses The data were analyzed by ANOVA using SPSS software. Results are reported as mean ± standard deviation and p
2490 J. Med. Plants Res. Inhibition ratio (%) Figure 1. Inhibition ratio of S180 cell growth affected by different concentration LFP91-3. Table 2. Inhibition ratio of S180 cell growth affected by different concentration LFP91-3. Time (h) Inhibition ratio (%) 5 μg/ml 10 μg/ml 15 μg/ml 24 8.16 21.42 29.89 48 14.45 29.17 64.14 72 29.91 39.90 74.53 Table 3. Apoptosis ratio of S180 in the initial stage between LFP91-3 experimental group and NS control group. Time 0 24 48 72 Time (h) Apoptosis ratio in the initial stage (%) 24 h 48 h 72 h NS control group 0.82 0.93 1.22 LFP91-3 experimental group 2.56 7.51 8.47 the LFP91-3II-treatment group, in contrast, mortality was only 60%, with an average survival time of 28.5 days (12.7 days longer than the control group; p
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Journal of Medicinal Plants Researc
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Editors Prof. Akah Peter Achunike E
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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Table of Contents: Volume 6 Number
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Figure 1. Chuanxiong Rhizoma (http:
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Table 1. Identification of main pea
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Table 3. Pharmacokinetic parameters
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active component study, many invest
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and 1185 kg ha -1 in the first site
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Table 1. Recommendation for use fer
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Sadanandan AK, Hamza S (1996c). Stu
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and it has been traced to South Ame
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Table 3. Results of phytochemical a
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(a) (b) (c) Figure 1. (a) Normal ce
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emedies in the south of Brazil. J.
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known fatty acids and terpenoids we
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Table 1. Renal function tests of ra
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Al-Radahe et al. 2271 Figure 3.nnnn
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namely disruption of the vascular e
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Zayachkivska OS, Konturek SJ, Drozd
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β-carotene were purchased from Sig
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Figure 1. Effect of ethanol concent
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Table 3. Climatic and soil factors
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content of polysaccharides. Phospha
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Serge et al. 2285 Table 1. Relative
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Percentage inhibition % of inhibiti
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Table 1. Result of phytochemical sc
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Table 3. Result of thin layer chrom
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Table 2. MIC of a compound 1 from c
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Zirihi et al. 2301 Figure 2. Termin
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Figure 5. T. catappa Linn. (Combret
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Figure 8. A. fumigatus: Aspect of c
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Figure 12. Sensitivity of A. fumiga
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Noormi et al. 2311 Table 1. Callus
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Table 2. Contd. NoC=No callus. 4.0
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Figure 2. Contd. at 2.0 mg/L yellow
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Table 1. Biochemical parameters in
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Figure 3. Bcl-xL reactions in inter
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ACKNOWLEDGMENTS The authors would l
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Figure 1. The first page of the boo
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Figure 3. The most widely included
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Figure 4. Lithographic print “St.
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Table 1. List of plants included in
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Table 1. Contd. Nedelcheva 2333 Cin
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Table 1. Contd. Nedelcheva 2335 Rhe
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14 7 1 15 1 35 17 16 Imported plant
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Academy of Science Publishing House
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et al., 1996; van Wyk and Gericke,
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DPPH inhibition (%) DPPH % inhibiti
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% Mortality Concentration (µg/ml)
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information on plants used for the
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Table 1. Levels of independent vari
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Arbutin (mg/g) Co-solvent Figure 1.
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Arbutin (mg/g) Extraction pressure
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Table 3. Arbuitn content of Asian p
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Arbutin (mg/g) Extraction pressure
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Arbutin (mg/g) Extraction temperatu
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Table 1. Chemical composition of es
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Darjazi 2369 Table 2. Statistical a
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Table 3. Correlation matrix (number
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Table 1. Precision test. extraction
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Figure 2. Effect of different alcoh
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Table 6. Results of ANOVA. Nie et a
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Table 2. Plant data and MIC values
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exhibiting the polar extracts of P.
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Table 3. Contd. AcOEt ++ ++ ++ - Me
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Figure 1. Map of Ladakh region of I
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Warghat et al. 2391 Table 3. Summar
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Table 5. Inter-Population genetic i
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Excoffier L, Smouse PE, Quattro JM
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ice residue is an ideal raw materia
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DPPH• scavenging ratio(%) OD valu
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scavenging effect on free radical a
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our new CL system. Fenton reaction
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1 2 Time (s) Figure 2. Kinetic curv
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Wong et al., 2006). It can be seen
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Pan YM, Liang Y, Wang HS, Liang M (
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2422 J. Med. Plants Res. close cano
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2424 J. Med. Plants Res. Table 1. M
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2426 J. Med. Plants Res. Table 1. C
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2432 J. Med. Plants Res. Table 2. D
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2434 J. Med. Plants Res. Table 6. T
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2436 J. Med. Plants Res. budget dur
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