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Journal of Medicinal Plants Research Vol. 6(12), pp. 2488-2492, 30 March, 2012 Available online at http://www.academicjournals.org/JMPR DOI: 10.5897/JMPR11.1765 ISSN 1996-0875 ©2012 <strong>Academic</strong> <strong>Journals</strong> Full Length Research Paper The antitumor activities of Lentinula edodes C91-3 mycelia fermentation protein on S180 (Mouse sarcoma cell) in vivo and in vitro Mintao Zhong, Ben Liu, Yingli Liu, Xiaoli Wang, Xingyun Li, Lei Liu, Anhong Ning, Jing Cao and Min Huang* Department of Medical Microbiology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, China. Accepted 16 February, 2012 Lentinula edodes is one of the most widely used medicinal mushrooms which exhibit significant antitumor activity. It is mostly studied and several studies have showed that its polysaccharides play critical roles in the antitumor effects. In this study, we determine the antitumor activity of Lentinula edodes C91-3 mycelia fermentation protein (LFP91-3) on S180 tumor cells in vivo as well as in vitro. The results showed that LFP91-3 inhibited the growth of S180 tumor cells in vitro, and the mechanism may be related to apoptosis induced by LFP91-3. In vivo, LFP91-3 treatment significantly prolonged life span in S180 tumor-bearing mice. LFP91-3 can be used as an important antitumor element in Lentinula edodes C 91-3 mycelia fermentative liquid. Key words: Lentinula edodes C91-3 fermentation protein, Lentinula edodes, antitumor activity. INTRODUCTION Lentinula edodes, commonly known as the Shiitake mushroom, is the second most popular and widely cultivated edible mushroom in the world. As the main medicinal fungi, its extract has anti-viral, anti-bacteria, anti-tumor activity and regulation of immune function, etc (Hearst et al., 2009; Kuppusamy et al., 2009; Sarangi et al., 2006; Unursaikhan et al., 2006). The strain of Lentinula edodes was studied in March, 1991. So we named Lentinula edodes C91-3, and “C” means China. Numerous studies showed Lentinan could inhibit tumor cells growth, improve patient’s symptoms, and reduce adverse reactions. It is now officially used for clinical medicine as adjuvant chemotherapy drugs (Ooi and Liu, 2000; Kidd, 2005; Zheng et al., 2005; Fang et al., 2006; Miyaji et al., 2006). However, antitumor activity of Lentinula edodes on protein level was rarely reported. In this study submerged fermentation for Lentinula edodes C91-3 mycelia was done and its antitumor effects on protein level were determined. *Corresponding author. E-mail: huangminchao@163.com. Tel:+86-411-86110007. Fax: +86-411-86110007 MATERIALS AND METHODS Experimental animals 50 healthy inbred BALB/C mice were selected. Three months old SPF-grade mice were used having weight 18 to 20 g. We used 20 male and 20 female mice, obtained from the Experimental Animal Center of Dalian Medical University. This study was approved by Dalian Medical University Animal Ethical Review Committee, similar to the guidelines for the use and care of animals that are published by the National Institute of Health. Carcinoma cell lines S180 (mouse sarcoma tumor) cell lines were obtained from the Department of Microbiology of Dalian Medical University. Cell lines were grown in RPMI-1640 medium (Gibco) containing 10% fetal calf serum (China National Medicines Corporation, Ltd). The cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. Lentinula edodes C91-3 strain and culture conditions Lentinula edodes C91-3 strain were obtained from the Department of Microbiology of Dalian Medical University. It was cultured in the potato culture medium containing 1% Vitamin B1, 2.0% Agar, 0.15% MgSO4, 0.3% K2HPO4, and 2.0% glucose having pH 6.0.
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Journal of Medicinal Plants Researc
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Editors Prof. Akah Peter Achunike E
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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Table of Contents: Volume 6 Number
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Figure 1. Chuanxiong Rhizoma (http:
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Table 1. Identification of main pea
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Table 3. Pharmacokinetic parameters
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active component study, many invest
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and 1185 kg ha -1 in the first site
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Table 1. Recommendation for use fer
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Sadanandan AK, Hamza S (1996c). Stu
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and it has been traced to South Ame
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Table 3. Results of phytochemical a
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(a) (b) (c) Figure 1. (a) Normal ce
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emedies in the south of Brazil. J.
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known fatty acids and terpenoids we
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Table 1. Renal function tests of ra
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Al-Radahe et al. 2271 Figure 3.nnnn
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namely disruption of the vascular e
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Zayachkivska OS, Konturek SJ, Drozd
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β-carotene were purchased from Sig
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Figure 1. Effect of ethanol concent
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Table 3. Climatic and soil factors
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content of polysaccharides. Phospha
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Serge et al. 2285 Table 1. Relative
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Percentage inhibition % of inhibiti
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Table 1. Result of phytochemical sc
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Table 3. Result of thin layer chrom
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Table 2. MIC of a compound 1 from c
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Figure 5. T. catappa Linn. (Combret
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Figure 8. A. fumigatus: Aspect of c
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Figure 12. Sensitivity of A. fumiga
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Noormi et al. 2311 Table 1. Callus
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Figure 2. Contd. at 2.0 mg/L yellow
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Table 1. Biochemical parameters in
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Figure 3. Bcl-xL reactions in inter
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ACKNOWLEDGMENTS The authors would l
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Figure 1. The first page of the boo
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Figure 3. The most widely included
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Figure 4. Lithographic print “St.
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Table 1. List of plants included in
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Table 1. Contd. Nedelcheva 2333 Cin
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Table 1. Contd. Nedelcheva 2335 Rhe
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14 7 1 15 1 35 17 16 Imported plant
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Academy of Science Publishing House
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et al., 1996; van Wyk and Gericke,
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DPPH inhibition (%) DPPH % inhibiti
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% Mortality Concentration (µg/ml)
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information on plants used for the
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Table 1. Levels of independent vari
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Arbutin (mg/g) Co-solvent Figure 1.
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Arbutin (mg/g) Extraction pressure
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Table 3. Arbuitn content of Asian p
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Arbutin (mg/g) Extraction pressure
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Arbutin (mg/g) Extraction temperatu
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Table 1. Chemical composition of es
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Darjazi 2369 Table 2. Statistical a
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Table 3. Correlation matrix (number
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Table 1. Precision test. extraction
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Figure 2. Effect of different alcoh
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Table 6. Results of ANOVA. Nie et a
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Table 2. Plant data and MIC values
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exhibiting the polar extracts of P.
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Table 3. Contd. AcOEt ++ ++ ++ - Me
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Figure 1. Map of Ladakh region of I
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Warghat et al. 2391 Table 3. Summar
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Table 5. Inter-Population genetic i
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Excoffier L, Smouse PE, Quattro JM
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ice residue is an ideal raw materia
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DPPH• scavenging ratio(%) OD valu
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scavenging effect on free radical a
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our new CL system. Fenton reaction
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1 2 Time (s) Figure 2. Kinetic curv
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Wong et al., 2006). It can be seen
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Pan YM, Liang Y, Wang HS, Liang M (
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