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(C-5), 41.3(C-3), 37.9 (C-1), 37.8 (C-10), 35.9 (C- 6),33.2 (C-4), 32.5 (C-2), 23.2 (C-18), 21.3 (C-19), 18.9 (C-20)。IR (KBr, cm -1 ): 3428, 2990,2915, 2820, 1645, 1590, 1505, 1430, 1355, 1323, 1278, 1188, 875. This spectral data of 1 H NMR and 13 C NMR are in agreement with that of nimbidiol in the literature (Majumder et al., 1987; Masahiro et al., 2010). Data of compound I molecular formula C30H40O4. mp 217 to 219°C. EI- MS m/z 487[M+Na] + , 465[M+H] + 。IR (KBrυmax / cm -1 ): 3350, 2940, 1723, 1650, 1590, 1520, 1435, 1375,1300,1245,1220, 1205, 1185, 155, 1145, 1095, 1085, 990, 870, 860, 850。 1 H-NMR (CDCl3): δ 7.03 (1H, d, J=9.2Hz, H-6), 6.98 (1H, s, Figure 2. The HPLC chromatogram of compound II. Column: Waters symmetry C18 column (150×4.6 mm); column temperature: 25°C; mobile phase: methanol: 0.1%phosphoric acid (88:12), flow rate: 0.8 ml/ min; detection wavelength: 254 nm. H-1), 6.36 (1H, d, J=7.2Hz, H-7), 6.53 (1H, brs, disappearing after the exchange of D2O, -OH), 3.55 (3H, s, H-31), 2.22 (3H, s, H-23), 1.45 (3H, s, H-25),1.26 (3H, s, H-26), 1.18 (3H, s, H-30), 1.08 (3H, s, H-28), 0.52 (3H, s, H-27)。 13 C-NMR:δ 119.8 (C-1), 178.6 (C-2), 146.2 (C-3), 117.3 (C-4), 127.6 (C-5), 133. 2(C-6),118.3 (C-7) 160.3 (C-8), 43.2 (C-9) 164.9 (C-10) 33.8 C-11) 29.9 (C-12) 39.6 (C-13), 45.3 (C-14), 28.9 (C-15), 36.6 (C-16), 30.8 (C-17), 44.5(C-18), 31.1 (C-19),40.6 (C- 20), 30.1 (C-21), 35.0 (C-22), 10.5 (C-23), 38.5 (C-25), 21. 8(C-26), 18.5 (C-27), 31.8(C-28), 178.9 (C-29), 32.9 (C-30), 51.8 (C-31). This spectral data of 1 H NMR and 13 C NMR are in agreement with that of pristimerin in the literature (Jiang et al., 1996). CONCLUSION Xie et al. 2523 HSCCC was successfully used for the preparative isolation of terpenoids. In combination with a suitable extraction and cleanup procedure prior to HSCCC separation, 2.5 mg nimbidiol and 3.0 mg pristimerin were obtained from 250 mg of the crude sample on a preparative scale. Meanwhile, the isolation of nimbidiol and pristimerin for the first time from C. aculeatus Merr proved HSCCC to be a useful tool for the exploring of the chemical component of traditional medicinal herbs.
2524 J. Med. Plants Res. ACKNOWLEDGEMENTS Figure 3. HSCCC chromatogram of crude extract from C. aculeatus Merr. Two-phase solvent system, n-hexane-ethyl acetate-methanol-water (2.3:2:2:1.3 v/v); mobile phase, the lower phase; flow rate, 2.0 mL/min; revolution speed, 900rpm; detection wavelength, 254 nm; separation temperature, 20°C; sample size, 250 mg crude sample dissolved in 10 mL of the upper phase and 10 ml of the lower phase. Retention of the stationary phase: 67% This project was supported by the National Centerfor Complementary and Alternative Medicine of NIH (No. FO5AT002013-03) and was partly supported by Fund for Social Development Plan Projects of Technology Department of Guang dong province, No. 2006B35604001, Guangzhou Technology Projects, No.2007JC0081. The authors would like to thank South China Agricultural University for the measurement of infrared (IR), NMR spectra and EI-MS. REFERENCES Guo YQ, Li X, Xu J, Li N, Meng DL, Wang JH (2004) . Sesquiterpene esters from the fruits of Celastrus orbiculatus. Chem. Pharm. Bull. (Tokyo), 2: 1134-1136. Ito Y (1981). Efficient preparative counter-current chromatography with a coil planet centrifuge.Journal of Chromatogr. A, 214(1): 122-125. Ito Y (2003).Technical guidance for high-speed counter-current chromatography, Foods Food Ingredients J. Jpn., 208(9): 709-724. Ito Y (2005). Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography.J. Chromatogr. A, 065(2): 145-168. Jin HZ, Hwang BY, Kim HS, Lee JH, Kim YH, Lee JJ (2002) . Antiinflammatory constituents of Celastrus orbiculatus inhibit the NF-kappaB activation and NO production. J. Nat. Prod., 65: 89-91. Jiang Yi, Li He, Luo SQ (1996). Progress of studies on effective composition from Celastrus orbiculatus Thunb. Chin.
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Journal of Medicinal Plants Researc
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Editors Prof. Akah Peter Achunike E
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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Table of Contents: Volume 6 Number
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Figure 1. Chuanxiong Rhizoma (http:
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Table 1. Identification of main pea
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Table 3. Pharmacokinetic parameters
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active component study, many invest
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and 1185 kg ha -1 in the first site
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Table 1. Recommendation for use fer
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Sadanandan AK, Hamza S (1996c). Stu
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and it has been traced to South Ame
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Table 3. Results of phytochemical a
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(a) (b) (c) Figure 1. (a) Normal ce
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emedies in the south of Brazil. J.
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known fatty acids and terpenoids we
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Table 1. Renal function tests of ra
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Al-Radahe et al. 2271 Figure 3.nnnn
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namely disruption of the vascular e
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Zayachkivska OS, Konturek SJ, Drozd
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β-carotene were purchased from Sig
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Figure 1. Effect of ethanol concent
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Table 3. Climatic and soil factors
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content of polysaccharides. Phospha
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Serge et al. 2285 Table 1. Relative
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Percentage inhibition % of inhibiti
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Table 1. Result of phytochemical sc
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Table 3. Result of thin layer chrom
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Table 2. MIC of a compound 1 from c
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Zirihi et al. 2301 Figure 2. Termin
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Figure 5. T. catappa Linn. (Combret
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Figure 8. A. fumigatus: Aspect of c
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Figure 12. Sensitivity of A. fumiga
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Noormi et al. 2311 Table 1. Callus
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Table 2. Contd. NoC=No callus. 4.0
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Figure 2. Contd. at 2.0 mg/L yellow
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Table 1. Biochemical parameters in
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Figure 3. Bcl-xL reactions in inter
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ACKNOWLEDGMENTS The authors would l
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Figure 1. The first page of the boo
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Figure 3. The most widely included
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Figure 4. Lithographic print “St.
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Table 1. List of plants included in
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Table 1. Contd. Nedelcheva 2333 Cin
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Table 1. Contd. Nedelcheva 2335 Rhe
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14 7 1 15 1 35 17 16 Imported plant
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Academy of Science Publishing House
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et al., 1996; van Wyk and Gericke,
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DPPH inhibition (%) DPPH % inhibiti
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% Mortality Concentration (µg/ml)
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information on plants used for the
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Table 1. Levels of independent vari
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Arbutin (mg/g) Co-solvent Figure 1.
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Arbutin (mg/g) Extraction pressure
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Table 3. Arbuitn content of Asian p
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Arbutin (mg/g) Extraction pressure
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Arbutin (mg/g) Extraction temperatu
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Table 1. Chemical composition of es
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Darjazi 2369 Table 2. Statistical a
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Table 3. Correlation matrix (number
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Table 1. Precision test. extraction
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Figure 2. Effect of different alcoh
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Table 6. Results of ANOVA. Nie et a
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Table 2. Plant data and MIC values
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exhibiting the polar extracts of P.
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Table 3. Contd. AcOEt ++ ++ ++ - Me
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Figure 1. Map of Ladakh region of I
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Warghat et al. 2391 Table 3. Summar
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Table 5. Inter-Population genetic i
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Excoffier L, Smouse PE, Quattro JM
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ice residue is an ideal raw materia
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DPPH• scavenging ratio(%) OD valu
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scavenging effect on free radical a
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our new CL system. Fenton reaction
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1 2 Time (s) Figure 2. Kinetic curv
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Wong et al., 2006). It can be seen
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Pan YM, Liang Y, Wang HS, Liang M (
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2422 J. Med. Plants Res. close cano
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2424 J. Med. Plants Res. Table 1. M
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2432 J. Med. Plants Res. Table 2. D
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2434 J. Med. Plants Res. Table 6. T
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2436 J. Med. Plants Res. budget dur
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2440 J. Med. Plants Res. Table 1. R
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2442 J. Med. Plants Res. Stability
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2444 J. Med. Plants Res. production
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2446 J. Med. Plants Res. Figure 2.
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2450 J. Med. Plants Res. Table 2. A
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2452 J. Med. Plants Res. Ghasemi Pi
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2454 J. Med. Plants Res. Table 1. I
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2456 J. Med. Plants Res. Table 3. M
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2460 J. Med. Plants Res. Department
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2462 J. Med. Plants Res. Table 3. E
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2464 J. Med. Plants Res. Table 6. E
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2466 J. Med. Plants Res. AOAC (2000
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