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2314 J. Med. Plants Res. Figure 1. Contd. Figure 2. Induction of Hymenocallis littoralis callus cultures derived from bulb explants in MS basal medium + B5 vitamins, 3% (w/v) sucrose, 0.25% (w/v) Gelrite agar supplemented with different hormones concentration (0, 1, 2, 3, 4, 5 mg/L) after 30 days of culture. A (2,4-D), B (dicamba), C (picloram), D (NAA), E (IAA). The scale (1 cm = 0.5 cm) representing the explants above.
Figure 2. Contd. at 2.0 mg/L yellowish-white, soft, compact callus and torpedo in shape. Meanwhile, picloram at 3.0 mg/L produced white yellowish callus and soft callus appearance. NAA at 3.0 mg/L produced small callus appearance of white and yellowish in colour. No response was observed in the medium containing IAA, even the cultures were kept for prolonged eriod. A similar result was obtained by Das et al. (1995). The percentages of callus induction using various types of explants were found to be increased significantly by using selected types of auxin in Eurycoma longifolia plants. Conclusion The best auxin used for callus induction from root explants of H. littoralis was NAA at 2.0 mg/L followed by 2,4-D at 2.0 mg/L, picloram at 2.0 mg/L, IAA at 2.0 mg/L, and dicamba at 3.0 mg/L. Meanwhile, the best auxin used for callus induction from bulb explants of H. littoralis (Melong kecil) was 2,4-D at 2.0 mg/L, followed by Dicamba at 2.0 mg/L, picloram at 3.0 mg/L, and NAA at 2.0 mg/L. ACKNOWLEDGEMENTS This research was supported by Universiti Sains Malaysia, Malaysian Ministry of Higher Education (MOHE) and Universiti Teknologi MARA (UiTM) scholarship. REFERENCES Abou-Donia AH, Toaima SM, Hammoda HM, Kinoshita E, Takayama H Noormi et al. 2315 (2008). Pytochemical and Biological Investigation of Hymenocalli littoralis SALISB. Chem. Biodivers., 5(2): 332-340. Das AB, Das P, Rout GR (1995). In vitro somatic embryogenesis from callus culture of the timber yielding tree Hardwickia binata Roxb. Plant Cell Rep., 15: 147-149. Hippolyte I, Marin B, Baccou JC, Jonard R (1992). Growth and rosmarinic acid Production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep., 11: 109-112. Holme IB, Petersen KK (1996). Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda ‘Gingateus’. Plant Cell Tissue Org. Cult., 45: 43-52. Ji Z, Meerow AW (1985). Amaryllidaceae. Flora China, 24: 264. Lin LZ, Hu SF, Chai HB, Pengsuparp T, Pezzuto JM, Cordell GA, Ruangrungsi N (1995). Lycorine Alkaloids From Hymenocallis littoralis. Phytochemistry, 40(4): 1295-1298. Luczkiewicz M, Glod D (2003). Callus cultures of Genista plant-in vitro material producing high amounts of isoflavones of phytoestrogenic activity. J. Plant Sci., 165: 1101-1108. Manickam VS, Mathavan RE, Antonisamy R (2000). Regeneration of Indian ginseng plantlets from stem callus. Plant Cell Tiss. Org., 62: 181-185. Maziah M, Rosli N, Sreeramanan S (2010). Optimization of suitable auxin Application in a recalcitrant Woody forest plant of Eurycoma longifolia (Tongkat Ali) for callus induction. Afr. J. Biotechnol., 9(49): 8417-8428. Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant., 15: 473-477. Nagamori E, Hiraoka K, Honda H, Kobayashi T (2001). Enhancement of anthocyanin production from grape (Vitis vinifera) callus in a viscous additive-supplemented medium. Biochem. Eng. J., 9: 59-65. Rafael O, Michael J (2009). Plants of Semillas Sagradas: An Ethnomedical Garden in Costa Rica. Rev. Cubana Plant Med., 4(3): 61-62. Rashida CS, Rabia AM (2007). Establishment of Callus and Suspension Culture in Jatropha Curcas. J. Bot., 39(7): 2431-2441 Renard-Noiaki JT, Kim Y, Imakura M, Kihara KS (1989). Effect of alkaloids isolated from Amaryllidaceae on Herpes-Simplex virus. Res. Virol., 140: 115-128 Rosli N, Maziah M, Chan KL, Sreeramanan S (2009). Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. J. For. Res., 20(1): 54-58. Sierra MI, Heijden R, Schripsema J, Verpoorte R (1991). Alkaloid production in relation to differentiation in cell and tissue cultures of Tabernaemontana pandacaqui. Planta Med., 57: 543-547.
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Journal of Medicinal Plants Researc
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Editors Prof. Akah Peter Achunike E
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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Table of Contents: Volume 6 Number
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Figure 1. Chuanxiong Rhizoma (http:
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Table 1. Identification of main pea
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Table 3. Pharmacokinetic parameters
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active component study, many invest
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and 1185 kg ha -1 in the first site
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Table 1. Recommendation for use fer
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Sadanandan AK, Hamza S (1996c). Stu
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and it has been traced to South Ame
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Table 3. Results of phytochemical a
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Table 1. Chemical composition of es
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Darjazi 2369 Table 2. Statistical a
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Table 1. Precision test. extraction
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Figure 2. Effect of different alcoh
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Table 2. Plant data and MIC values
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exhibiting the polar extracts of P.
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Figure 1. Map of Ladakh region of I
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Warghat et al. 2391 Table 3. Summar
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Table 5. Inter-Population genetic i
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Excoffier L, Smouse PE, Quattro JM
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ice residue is an ideal raw materia
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DPPH• scavenging ratio(%) OD valu
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scavenging effect on free radical a
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our new CL system. Fenton reaction
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1 2 Time (s) Figure 2. Kinetic curv
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Wong et al., 2006). It can be seen
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Pan YM, Liang Y, Wang HS, Liang M (
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2422 J. Med. Plants Res. close cano
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2442 J. Med. Plants Res. Stability
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2486 J. Med. Plants Res. components
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UPCOMING CONFERENCES 15th European
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