THESE Anne POSTEC Diversité de populations microbiennes ...

Résultats - Chapitre 3
Figure 3. Whole-cell hybridization of fixed cells from 3 samples (T2, T7 and T28) collected
from the enrichment culture in bioreactor. Cells were stained with DAPI (T2A, T7A and T28
A), and hybridized with the FITC-labeled Eub338 probe (T7B), or the Cy-3-labeled Arch 915
(T2B and T28B). Cells were viewed by epifluorescence microscopy in which DAPI-, FITCand
Cy-3-specific filters were used. The relative proportions of archaeal and bacterial cells
were determined by counting approximately 2000 cells on filter and resulted in: 99.0% of the
cells detected at T2 belonged to the Archaea (see T2B), 99.0% of the cells detected at T7
belonged to the Bacteria (see T7B), 40% of the cells detected at T28 belonged to the
Archaea (see T28B). Scale bars = 10 µm.
194