Book of Abstracts (PDF) - International Mycological Association

IMC7 Friday August 16th Lectures
some substrates that are unsuitable for direct use as human
food and/or animal feed, while concomitantly destroying
hazardous substances they eventually contain, and
operating an efficient bioremediation.
361 - Development of functional foods by use of
basidiomycetal strains
J.S. Lee 1* , H.C. Ha 1 , S.Y. Kim 1 , H.J. Kim 1 & K.B. Park 2
1 Dept.of Bioindustry,Yeungnam University, 214-1
Daedong,Gyungsan Gyungbuk, Korea. - 2 Haechandle Co.,
284-3 Yongmundong Seogu Daejeon, Korea. - E-mail:
jslee@yumail.ac.kr
In this study, it was attempted to investigate the
hepatoprotective effects of two fractions of methanol
extracts, larger molecular fraction(above 20,000 MW) and
smaller molecular fraction(under 20,000 MW), obtained
from waxy brown rice cultured with Phellinus igniarius.
After administering these sample fractions to rats orally for
2 consecutive weeks, rats were treated with CCL4. The
determination of enzyme activities in sera of treated rats as
well as biochemical analysis of liver were conducted and
compared with those data from control group. Serum GOT
and GPT of rats increased highly in the control group
treated with CCL4 only. The treatment group fed with
larger molecular fraction of the methanol extracts obtained
from waxy brown rice cultured with P. igniarius showed a
strong suppression on the GOT and GPT augmentation. In
the group treated with only CCL4, all the activities of ALP,
LDH, γ-GTP, SDH increased considerably compared to the
normal rats without CCL4 treatment. However, both the
groups fed with methanol extracts from brown rice cultured
with P. igniarus prevented the increase in activities of
these enzymes, almost to the level of normal rats without
CCL4 treatment in some of the data. The larger molecular
fraction demonstrated higher effectiveness in general.
362 - High incidence of mycotoxin zearalenone in stored
wheat from Mazandaran Province, north of Iran
M.T. Hedayati * & A.R. Dalili
Faculty of Health, Amir Mazandarani Boul. Vesal Shirazi
St. Post code 48167, Sari, Iran. - E-mail:
hedayaty2001@yahoo.co.uk
In Iran, wheat is one of the main cereals for human
consumption. Wheat grains containing zearalenone can
cause genital problem and reproductive disorders in
domestic animals. In human, episodes of breast
enlargement in young boys and sexual precocity have been
reported. This toxin may therefore have a role in hormonal
balance and mammary cancer in regions with high
zearalenone ingestion. In this study the incidence of
zearalenone in wheat samples from Mazandaran
warehouses were investigated. A total of 118 wheat
samples (1 kg) were randomly collected from all 12
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Book of Abstracts
warehouses of Mazandaran province. Competitive
microtitre plate enzyme immunoassay for the quantitative
determination of zearalenone was used according the
manufacturer instructions (Sigma-Aldrich
Laborchemikalien GmbH). Zearalenone was found in 81%
of samples and 64.4% of samples contained >200 µg/kg of
this toxin. The minimum levels of zearalenone were 29
µg/kg. All of the warehouses were positive for the presence
of zearalenone. 50-100% of samples in each of warehouse
had >200 µg/kg contamination of zearalenone. Our results
showed the high percentage and levels of contamination of
wheat samples with zearalenone. With regard to proposed
standards given by the Standard Administration of Iran,
under which the content of zearalenone should not exceed
200 ppb in food products, 64.4% of samples meet these
criteria.
363 - Mycelial growth of Ganoderma lucidum and
Pleurotus ostreatus on the selenium-enriched media
M. Stajic 1* , I. Brceski 2 , M. Kezman 1 , S. Duletic-Lausevic 1
& J. Vukojevic 1
1 Faculty of Biology; University of Belgrade, Takovska 43;
11000 Belgrade, Yugoslavia. - 2 Faculty of Chemistry;
University of Belgrade, Studentski trg 3; 11000 Belgrade,
Yugoslavia. - E-mail: stajicm@hotmail.com
Ganoderma lucidum and Pleurotus ostreatus are
mushrooms of high pharmacological value. 119 different
kinds of triterpenoids and various polysaccharides from G.
lucidum fruiting bodies have shown antitumor and
immunostimulating effects. Polysaccharides isolated from
P. ostreatus have shown antitumor, immunomodulating,
antiviral, antibacterial activities. Selenium is one of the
essential oligoelements, and it is indispensable for cell
functions. Selenium adding in nutrition has been shown to
have anticancer and antimutagenic effects. The aim of our
research was to investigate the influence of selenium added
in media on the growth rate and biomass changes of
mycelia and them ability to accumulate this microelement.
The medium used for analyzing the growth rate of the
mycelia was Malt agar. The same submerged medium was
used for measuring mycelial biomass and the accumulated
selenium. The concentrations of selenium, used in a form
of sodium selenite, were the following: 0.3 mg/l, 0.7 mg/l,
1 mg/l and 1.3 mg/l. The accumulation of selenium in
mycelia was measured using the Atomic Absorption
Spectrophotometer. The analyzed concentrations of
selenium did not show any influence to the growth rate of
both species mycelia in comparison to the control.
Different concentrations of selenium showed influence on
mycelial biomass in submerged culture. The results of
selenium accumulation in mycelia have shown higher
concentration in samples grown on selenium containing
media.