Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
starch-like compounds, assimilation of D-glucuronic and/or
protocatechuic acids but not inositol. These yeasts were
assigned to the Erythrobasidium lineage of the
Urediniomycetes using a combination of conventional
phenotypic identification methods with molecular
techniques: PCR fingerprinting (MSP-PCR) with primer
m13 and rDNA sequence analysis (26S rDNA, D1/D1
domains). The results showed that some of the isolates
corresponded, or were similar, to recognised species, viz.
Sporobolomyces coprosmae, Sp. salicinus, Sp. gracilis,
Rhodotorula aurantiaca and Erythrobasidium
hasegawianum, whereas others seemed to represent
undescribed species.
690 - Evolution of mating type gene arrangement in the
genus Stemphylium (Ascomycetes)
P. Inderbitzin * , J. Harkness & M.L. Berbee
University of British Columbia, Department of Botany,
#3529-6270 University Blvd., Vancouver, BC V6T 1Z4,
Canada. - E-mail: bhpatrik@mail.botany.ubc.ca
In this study we are investigating distribution and
arrangement of the mating type genes MAT-1 and MAT-2
in 96 isolates of the genus Stemphylium. The mating types
were assessed with primer sets amplifying the diagnostic
alpha and HMG boxes of the MAT-1 and MAT-2 genes
respectively. For most of the isolates, both MAT-1 and
MAT-2 genes could be demonstrated. In the genus
Cochliobolus, which is closely related to Stemphylium, the
occurrence of both mating type genes in one isolate is
indicative of homothallism. In homothallic species of
Cochliobolus, MAT-1 and MAT-2 are arranged in different
ways. In Stemphylium, several species are known
homothallics, including S. vesicarium, and the anamorph of
the type species of Pleospora, S. herbarum. MAT-1 and
MAT-2 were present in both S. vesicarium and S.
herbarum. The remaining 94 isolates included in this study
belonged to 17 other species, as well as a number of yet
undescribed species. Some of these isolates contained only
one mating type gene. The distribution of mating type
genes and their arrangement was mapped into phylogenetic
trees inferred from ITS, partial GPD, and EF-1a DNA
sequences. It is generally thought that homothallism
derived from heterothallism. To test this hypothesis, we
used phylogenetic analyses to investigate whether
heterothallism or homothallism was the ancestral state in
Stemphylium, and how many times a switch from
homothallism to heterothallism or vice versa occurred.
691 - A preliminary catologue of the Saprolegniaceae of
the Iberican peninsula
E.P. Iniesta 1* , M.T. Tellería 1 & M. Dueñas 2
1 Real Jardín Botánico de Madrid, CSIC, Plaza de Murillo
2, Madrid 28014, Spain. - 2 Universidad Autónoma de
Madrid., Ctr.a Colmenar viejo, km 15, Madrid 28049,
Spain. - E-mail: epiniesta@ma-rjb.csic.es
The Saprolegniaceae are one of the lest known and
investigated group of aquatic fungi in the Iberican
Peninsula. According to the bibliographic data it was only
known two genera over twenty of the orden. In view of the
interest concerning to the data in this area, we have been
undertaken a biosystematic study of the Saprolegniaceae in
the Iberican Peninsula, as a Phd included in the project
'Flora Micológica Iberica'. Up to now samples has been
colleted from different areas of West, North-East and South
of Iberican Peninsula and species from five genera of these
orden have been identificated: Saprolegnia, Achlya,
Dyctiuchus, Aphanomyces and Thraustotheca.
692 - Molecular and morphological comparisons of
Fusarium species accommodated in the Fusarium
subglutinans sensu lato complex
A. Jacobs 1 , E.T. Steenkamp 1 , T.A. Coutinho 1* , G.
Ramotshodi 2 , B.D. Wingfield 2 & M.J. Wingfield 1
1 Dept of Microbiology and Plant Pathology, Forestry and
Agricultural Biotechnology Institute (FABI), University of
Pretoria, Pretoria, 0002, South Africa. - 2 Dept of Genetics,
Forestry and Agricultural Biotechnology Institute (FABI),
University of Pretoria, Pretoria, 0002, South Africa. - Email:
teresa.coutinho@fabi.up.ac.za
A total of 13 species formed part of the Fusarium
subglutinans sensu lato species complex at the time of this
study. These species are pathogens of many hosts including
pine, mango and pineapple but are morphologically similar.
They are all characterised by typical F. subglutinans
morphology including false heads, absence of
chlamydospores and microconidia produced in
polyphialides. The aim of the study was to distinguish
between these species based on morphology and DNA
sequences. Phylogenetic relationships were determined
based on sequences from the histone, elongation 1α, and βtubulin
genes. Furthermore, sequences of the EF-1α gene
were subjected to restriction analysis using four different
restriction enzymes. Based on their recognition sites,
unique EF-1α restriction patterns were generated for the
species. The morphological characteristics that were
informative included the origin of conidiophores on the
aerial mycelium, conidiophore branching, the number of
conidiogenous openings on the polyphialides,
macroconidial septation and the presence or absence of
sterile coiled hyphae. All 13 of the species in the Fusarium
subglutinans sensu lato could thus be distinguished based
on morphological and molecular characteristics.
693 - Ophiostoma kryptum, prov. nom. from larch and
spruce in Europe, similar to Ophiostoma minus
K. Jacobs 1* & T. Kirisits 2
1 Eastern Cereal and Oilseed Research Centre, Agriculture
and Agri-Food Canada, 960 Carling Avenue, Ottawa,
Ontario, K1A 0C6, Canada. - 2 Institute of Forest
Entomology, Forest Pathology and Forest Protection,
Book of Abstracts 209