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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme IV: POPULATION DYNAMICS AND ECOLOGY Posters<br />

932 - Ligninolytic enzymes and biodegradation during<br />

the interactions <strong>of</strong> white-rot fungi and soil<br />

microorganisms<br />

P. Baldrian<br />

Institute <strong>of</strong> Microbiology AS CR, Videnska 1083, CZ-<br />

14220, Prague 4, Czech Republic. - E-mail:<br />

baldrian@biomed.cas.cz<br />

White-rot fungi are able to degrade lignin and related<br />

compounds. Under natural conditions, the process occurs in<br />

the presence <strong>of</strong> other microorganisms. Introduction <strong>of</strong><br />

microorganisms to liquid cultures <strong>of</strong> Trametes versicolor<br />

led to the increase <strong>of</strong> activity <strong>of</strong> the ligninolytic enzyme<br />

laccase. High increase was achieved with soil fungi<br />

Trichoderma harzianum (2810% <strong>of</strong> control), Penicillium<br />

rugulosum (1940%), Fusarium reticulatum (1690%),<br />

Cephalosporium sphaerospermum (840%), and Humicola<br />

grisea (480%). The increase was lower after addition <strong>of</strong><br />

bacteria - Escherichia coli (310%) and Bacillus subtilis<br />

(190%), or the yeast Endomyces magnusii (220%). After<br />

one-week cultivation with Trichoderma harzianum, the<br />

mycelium <strong>of</strong> T. versicolor was killed, which was<br />

accompanied by the decrease <strong>of</strong> Mn-peroxidase activity.<br />

Increase <strong>of</strong> laccase activity is a common response - it was<br />

found also in the white-rot fungi Abortiporus biennis,<br />

Coriolopsis occidentalis, Pleurotus ostreatus, Pycnoporus<br />

cinnabarinus and Trametes hirsuta. It might be involved in<br />

active defence, since some products <strong>of</strong> laccase exhibit<br />

antimicrobial activities. Increase <strong>of</strong> laccase activity<br />

correlated with the increase <strong>of</strong> decolorization <strong>of</strong> the<br />

synthetic dye Remazol brilliant blue R. It seems, that<br />

interspecific interactions can affect the biodegradative<br />

activity <strong>of</strong> white-rot fungi in situ. This work was supported<br />

by the Grant Agency <strong>of</strong> the Czech Academy <strong>of</strong> Sciences<br />

(B5020202).<br />

933 - Population structure <strong>of</strong> Ceratocystis fimbriata<br />

from Congo, Colombia and Uruguay, determined using<br />

microsatellite markers<br />

I. Barnes 1* , J. Roux 1 , B.D. Wingfield 2 & M.J. Wingfield 1<br />

1 University <strong>of</strong> Pretoria, Forestry and Agricultural<br />

Biotechnology Institute, Department <strong>of</strong> Microbiology and<br />

Plant Pathology, 74 Lunnon Road, FABI, University <strong>of</strong><br />

Pretoria, Pretoria, 0002, South Africa. - 2 University <strong>of</strong><br />

Pretoria, FABI, Department <strong>of</strong> Genetics, 74 Lunnon Road,<br />

FABI, University <strong>of</strong> Pretoria, Pretoria, 0002, South Africa.<br />

- E-mail: irene.barnes@fabi.up.ac.za<br />

Ceratocystis fimbriata is a haploid ascomycete that causes<br />

serious diseases on a wide range <strong>of</strong> plants, world-wide.<br />

Very little is known regarding the population biology or<br />

origin <strong>of</strong> this important pathogen. The aim <strong>of</strong> this study<br />

was to use 11 polymorphic PCR-based microsatellite<br />

markers previously designed for C. fimbriata, to examine<br />

the population structure and genetic diversity for different<br />

populations <strong>of</strong> C. fimbriata. Populations consisting <strong>of</strong> 32<br />

isolates from each <strong>of</strong> Congo, Colombia and 22 from<br />

Uruguay were studied. High genetic diversities for all<br />

populations were observed (H = 0.48-0.31; G = 24-48%).<br />

Colombia was the most diverse population consisting <strong>of</strong><br />

82% <strong>of</strong> the total, and 53% <strong>of</strong> the unique, alleles. Genetic<br />

differentiation between populations was great (GST= 0.39)<br />

and minimal gene flow was observed (Nm = 0.77). I A,<br />

PTLPT, and linkage disequilibrium tests to determine<br />

mode <strong>of</strong> reproduction showed little evidence for<br />

recombination within populations. C. fimbriata appears to<br />

reproduce primarily without outcrossing. UPGMA<br />

dendrograms showed that the Colombian population was<br />

more distantly related to the Congo and Uruguay<br />

populations than they were to each other. Some <strong>of</strong> the<br />

groups in the Colombian and Uruguay populations were<br />

genetically similar to isolates from the Congo. Results <strong>of</strong><br />

this study show that African isolates <strong>of</strong> C. fimbriata<br />

originated in Latin America. Moreover, evidence indicates<br />

that Latin America is also a likely area <strong>of</strong> origin for C.<br />

fimbriata.<br />

934 - Studies in the life cycle <strong>of</strong> Puccinia glechomatis<br />

J.B. Boellmann & M.S. Scholler *<br />

Purdue University, Department <strong>of</strong> Botany & Plant<br />

Pathology, Arthur & Kriebel Herbaria, Lilly Hall, West<br />

Lafayette, IN 47907, U.S.A. - E-mail: scholler@purdue.edu<br />

Puccinia glechomatis DC. is a microcyclic rust fungus on<br />

Glechoma spp. (Lamiaceae) forming only telia and basidia<br />

(spore states III, IV). The species is a native <strong>of</strong> Eurasia but<br />

naturalized in North America where it is spreading since<br />

the early 1990s. We studied the life cycle <strong>of</strong> the species by<br />

propagating the fungus on G. hederacea (ground-ivy) in<br />

the greenhouse, by various inoculation and germination<br />

techniques and by using the light microscope. Nuclear<br />

conditions were documented by epifluorescence<br />

microscopy and DAPI as DNA specific fluorochrome. The<br />

complete life cycle requires c. 20 d. After karyogamy in<br />

teliospores both cells germinate with a phragmobasidium.<br />

In basidia meiosis produces four nuclei, which move in the<br />

developing basidiospores. Further mitosis in the<br />

basidiospore may result in up to four nuclei. All but one<br />

nucleus degenerates during germination <strong>of</strong> the<br />

basidiospore. The germ tube penetrates the epidermis on<br />

the upper leaf surface and develops a rich haploid<br />

mycelium especially around stomata. Fusion <strong>of</strong> haploid<br />

hyphae occurs could not be documented. Our studies<br />

indicate that the fungus is homothallic.<br />

935 - Preliminary study <strong>of</strong> NE Portugal's macr<strong>of</strong>ungal<br />

communities<br />

S.M. Branco * & A.P. Rodrigues<br />

Parque Natural de Montesinho/Instituto da Conservação<br />

da Natureza, Apartado 90, 5301 Bragança, Portugal. - Email:<br />

sarabranco@yahoo.com<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong> 281

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