Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme I: BIODIVERSITY AND CONSERVATION Posters
scarce. Most of study efforts have, therefore, been
concentrated to find infraspecific groupings such as formae
speciales or phytopathogenic races. A substantial
reinvestigation of Fusarium species has been conducted in
the last decade, according to the species concept based on
recent molecular-phylogenetic analyses and precise
comparative morphological studies. Presence of more than
40 species of Fusarium in Japan has been until now
recognized. Among them, F. fractiflexum, F. kyushuense
and F. nisikadoi were new species and F. circinatum, F.
concentricum, F. fujikuroi (= F. moniliforme MP-C), F.
globosum, F. incarnatum (= F. semitectum var. majus), F.
proliferatum (= F. moniliforme MP-D), F. phyllophylum,
F. verticillioides (= F. moniliforme s. str. = MP-A) and so
on were new records from Japan, including renaming.
459 - Physical and chemical effects of fungi and lichens
in rock weathering
C. Ascaso 1 & J. Wierzchos 2*
1 Centro de Ciencias Medioambientales, CSIC, c/ Serrano
115 dpdo, 28006 Madrid, Spain. - 2 Universitat de Lleida,
Servei de Microscòpia Electrònica, c/ Rovira Roure 44,
25198 Lleida, Spain. - E-mail: jacekw@suic-me.udl.es
The epilithic lichen thallus frequently contain microdivided
minerals and biominerals, meanwhile mycobiont hyphae
invade the rock surface. Within the rocks the protolichens
and hyphae constitutes an important part of the endolithic
biomass. The studies of organo-mineral phase constituted
by minerals and hyphae, algae, cyanobacteria and bacteria
should be perform by microscopy techniques that do not
disturb the biofilm structure. These techniques should
allow investigation of the internal ultrastructure of the
biotic components, leading to their identification. Such
techniques should also permit the observation of mineral
physical changes induced by microorganisms. The
equipment which allows the carrying out of this work is the
SEM operating in Backscattered mode (SEM-BSE). In situ
SEM-BSE (EDS) observation of the organo-mineral phase
allows detection of the biomobilisation processes and
minerals biotransformation. In Antarctic extreme
environment, biologically transformed minerals have been
observed, and as a result, diagenetic iron hydroxide
nanocrystals and clays have been found around
chasmoendolithic hyphae in Beacon sandstone. Also in
granitic rocks the biominerals, such as calcium oxalate and
silica deposit have been found close to the fungal cells. In
the biomobilisation processes as well as in the biomineral
formation, the fungi are involved leaving a distinct traces
of their microbial activity.
460 - Mycorrhizal fungi of seven Paphiopedilum species
in Thailand
P. Athipunyakom 1* , L. Manoch 2 & M. Tanticharoen 3
1 Plant Pathology and Microbiology Division, Department
of Agriculture, Chatuchuk, Bangkok 10900, Thailand. -
2 Department of Plant Pathology, Faculty of Agriculture,
Kasetsart University, Bangkok 10900, Thailand. -
3 National Center for Engineering and Biotechnology,
Bangkok 10400, Thailand. - E-mail:
pornpimon_a@yahoo.com
Healthy roots from mature plants of seven Paphiopedilum
species, namely Paphiopedilum concolor, P. concolor var.
striatum, P. exul, P. godefroyae, P. niveum, P. parashii and
P. villosum, were collected from various locations in
Thailand. Mycorrhizal fungi were isolated using a
modification of the Masuhara and Katsuya method. Root
pieces were washed with tap water and surface sterilized
for 5 min in 5% NaOHCl and then rinsed twice in sterile
distilled water. The root segments were then cut into
longitudinal sections and the epidermal layer was removed.
Cortical cells containing pelotons were macerated and
placed on 1/6 NDY medium and incubated for 3-7 days.
After the incubation period, hyphal tips were transferred to
PDA slants and kept as pure cultures. Identification was
based on morphological characters as observed under a
light microscope. Nuclei were stained with safranin O
using the Bandoni method. Three genera and six species of
mycorrhizal fungi were found as follow: Rhizoctonia
repens (P. concolor and P.exul), R. ramicola (P. exul),
Ceratorhiza goodyerae-repentis (P. godefroyae),
Epulorhiza calendulina (P. concolor var. striatum and P.
exul), Rhizoctonia sp.1 (P. parashii) and Rhizoctonia sp.2.
Nuclear staining revealed that all strains were binucleate
except Rhizoctonia sp.2 (P. niveum) which was
multinucleate. Pure cultures were maintained on slant
PDA, tissue paper, soil and liquid paraffin for further
studies on molecular phylogeny and symbiotic
germination.
461 - Canadian Collection of Fungal Cultures
(CCFC/DAOM
C. Babcock
Canadian Collection of Fungal Cultures, Eastern Cereal
and Oilseed Research Centre, Agriculture and Agri-Food
Canada, Rm 1015, K.W. Neatby Bldg., Ottawa, Ontario
K1A 0C6, Canada. - E-mail: babcockc@em.agr.ca
The culture collection maintains ˜ 11,000 plant pathogenic
and wood rotting fungal cultures representing ˜ 2,900
species. The catalogue can be viewed on the World Wide
WEB: http://sis.agr.gc.ca/brd/ccc. The CCFC is supported
by the taxonomic expertise of one of the largest groups of
systematic mycologist in North America, with
internationally recognized expertise in most major
taxonomic groups. Research is concentrated on solving
agricultural problems. The collection originated as an
amalgamation of individual working collections and now
serves as a primary Canadian repository for research
strains. It functions as a gene bank for this microbial
genetic resource and provides pure cultures to researchers
in agriculture, forestry, medicine, private industry and
biotechnology. Authenticates strains have been provided
for research programs involved in: crop resistance to fungal
diseases, biological control, diagnostic probe development
and identification, screening for new metabolites and
Book of Abstracts 141