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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme I: BIODIVERSITY AND CONSERVATION Posters<br />

microsporus, M. wynnei f. carpathicus, Melanoleuca<br />

polioleuca, Mycena zephirus, Panaeolus cintulus,<br />

Psathyrella microrhiza and Tubaria autochthona were<br />

recorded for the first time in Greece, while Ceratobasidium<br />

cornigerum, Mycoaciella bispora, Myxarium laccatum and<br />

Ramicola haustellaris represent first reports for the<br />

respective genera in Greece. Of particular interest are<br />

records <strong>of</strong> lignicolous species from Mediterranean<br />

evergreen scrubs and frygana, i.e. Hyphodontia juniperi<br />

and H. sambuci on Thymus capitatus; Peniophora lycii, P.<br />

pithya and P. tamaricicola on Pistacia lentiscus;<br />

Aleurodiscus dextrinoideocerussatus, Hyphodontia pruni,<br />

Peniophora lycii, Steccherinum fimbriatum and S.<br />

ochraceum on Genista acanthoclada; Aleurodiscus minor<br />

and Lentinellus omphalodes on Cistus sp.; Gloeophyllum<br />

abietinum, Perenniporia rosmarini and Peniophora piceae<br />

on Juniperus phoenicea; and Dacrymyces lacrymalis and<br />

Pluteus nanus f. nanus on Nerium oleander.<br />

558 - BIOTEC Culture Collection (BCC) in Thailand<br />

W. Potacharoen * , S. Sivichai & M. Tanticharoen<br />

BIOTEC - Culture Collection, National Center for Genetic<br />

Engineering and Biotechnology, National Science and<br />

Technology Development Agency, 113 Pahonyothin Rd..<br />

Khlong 1, Khlong Luang, Pathum Thani12120, Thailand. -<br />

E-mail: wanchern@biotec.or.th<br />

BIOTEC Culture Collection is a specialized collection<br />

formally established in 1996 under the National Science<br />

and Technology Development Agency (NSTDA). The<br />

principle objective is to provide safe deposition <strong>of</strong> cultures<br />

for researchers; to preserve and maintain microorganisms<br />

isolated from Thailand for future use; and to manage strain<br />

data information. Another major role is to supply cultures<br />

and related information for an in-house screening<br />

programme for drug discovery from microorganisms. The<br />

collection comprises 10,000 isolates fungi from various<br />

sources and habitats: insect fungi (1,618), aquatic<br />

freshwater and marine habitats (1,324), rice blast fungi<br />

(1,148), soil including leaf litter and humus (973), wooddecay<br />

Xylariaceae and Basidiomycota (898), plant seeds<br />

(389 strains), endophytic fungi (754), lichens (247) and<br />

taxa from other sources (1,090). The collection also<br />

includes bacteria and actinomycetes (1,789) and yeasts<br />

(342). The preservation methods used are freezing at -80<br />

°C, in a vapor phase <strong>of</strong> liquid nitrogen at -170 °C; freezedrying<br />

and under liquid paraffin oil. The essential strain<br />

data <strong>of</strong> cultures being maintained are used to create a<br />

required database and produce the catalogue <strong>of</strong> cultures.<br />

On-line database is now available at URL:<br />

http://bcc.biotec.or.th/.<br />

170<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong><br />

559 - Taxonomic verification <strong>of</strong> basidiomycetes cultures<br />

in the LE (BIN) collection<br />

N.V. Psurtseva<br />

Komarov Botanical Institute RAS, Pr<strong>of</strong>. Popov Str., 2, St.<br />

Petersburg, 197376, Russia. - E-mail:<br />

NadyaPsu@NP1512.spb.edu<br />

The Komarov Botanical Institute Basidiomycetes Culture<br />

Collection - LE (BIN) maintains over 1000 strains <strong>of</strong> 428<br />

species <strong>of</strong> aphyllophoroid, agaricoid and gasteromycetoid<br />

macromycetes collected mainly in Russia and former<br />

USSR. Conservation <strong>of</strong> Basidiomycetes diversity ex situ<br />

with a special interest in rare and endangered species as<br />

well as cultural maintenance <strong>of</strong> mushroom taxa with<br />

biological activity, especially those could be used in<br />

medicine and industry, is one <strong>of</strong> our primary goals.<br />

Taxonomic verification <strong>of</strong> Basidiomycetes cultures<br />

maintained in the culture collections is <strong>of</strong> great importance.<br />

In the process <strong>of</strong> starting and transferring living cultures<br />

error can be introduced in two ways. First, cultures can be<br />

obtained from incorrectly identified basidiomata or the<br />

identification has been done according to an out <strong>of</strong> date<br />

classification. Recent studies on Flammulina and Pleurotus<br />

species enable us to carry out taxonomic verification <strong>of</strong> LE<br />

(BIN) cultures from these genera by mating study<br />

according to the biological species concept. Second,<br />

replacement <strong>of</strong> a Basidiomycetes culture with a a different<br />

species, mainly from the group <strong>of</strong> imperfect fungi, can<br />

occur during the strain isolation or subculturing. This <strong>of</strong>ten<br />

happens in culturing <strong>of</strong> slowly growing and<br />

ectomycorrhizal fungi. To veryfy a cultural strain's identity<br />

and purity detailed studies <strong>of</strong> cultural characters, fruiting <strong>of</strong><br />

strains in culture and molecular analysis are being used in<br />

the LE(BIN) Collection.<br />

560 - Contribution <strong>of</strong> hyphal length in predicting P<br />

influx in maize and groundnut under field condition<br />

A. Rakshit 1* , P.B.S. Bhadoria 1 & N. Claassen 2<br />

1 Agri and Food Engg Dept., IIT, Kharagpur-721302, W.B,<br />

India. - 2 Agricultural Chemistry, University <strong>of</strong> Gottingen,<br />

University <strong>of</strong> Gottingen,Germany, Germany. - E-mail:<br />

amitabha_1975@yahoo.co.in<br />

When P is deficient in soil solution, the critical root<br />

parameter controlling its uptake is its surface area. Hyphae<br />

<strong>of</strong> mycorrhizal fungi have the potential to greatly increase<br />

the absorbing surface area <strong>of</strong> the root. Modified gridline<br />

intersect method was employed for extracting hyphae from<br />

the soil and quantifying length under field conditions in a<br />

Benomyl treated and non treated soil with the following<br />

three P levels: P 0,P 50 and P 400mgkg-1 soil as single<br />

super phosphate. To asses the importance <strong>of</strong> mycorrhizal<br />

hypha nutrient uptake model was used. Wettable powder <strong>of</strong><br />

the fungicide Benomyl was mixed thoroughly to 15 cm soil<br />

depth at 500 kg ha-1. Treated plots were covered by paddy<br />

straw for two weeks to avoid excessive heating. There was

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