Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme IV: POPULATION DYNAMICS AND ECOLOGY Posters
examined. We have developed a PCR-based assay to detect
strain MZ-340 reliably on bioremediation site. DNA,
isolated by series of CTAB and organic solvent extractions
followed by silica column purification from the site, was
amplified by the PCR using specific primer derived from
ITS sequences of the strain MZ-340. We successfully
monitored the transition of the population of the strain MZ-
340 on the bioremediation site. The relationship between
the population of the fungus and the effect of
bioremediation by this fungus will be discussed.
991 - Vegetative compatibility among Botrytis cinerea
strains in Israel
N. Korolev * , Y. Elad & T. Katan
Dept. Plant Pathol. ARO, the Volcani Center, P.O.Box 6,
Bet Dagan 50250, Israel. - E-mail: vpptlg@agri.gov.il
A total of 21 B. cinerea strains originating from five host
plants at 10 sites throughout Israel, were tested for mycelial
interaction. Complementation between auxotrophic
selenate-resistant mutants (sul) mutants, and antagonism
(barrage) between wild type strains served as criteria for
compatibility: Complementation indicates that the parent
strains are compatible, whereas the barrage indicates that
the strains are incompatible. sul mutants resistant or
sensitive to chromate were paired on MM. Wild type
growth of complementary heterokaryons was always
observed between different mutant phenotypes derived
from the same parent, as well as in some inter-strain
pairings. Most isolates were placed into the same group
based either on positive complementation reaction between
their mutants, or, more often, on positive reaction with
mutants of bridging strains, compatible with two or more
isolates that were not compatible directly. Barrage between
wild type isolates, in the form of a zone of sparse
mycelium with or without dark pigmentation of the agar
along the line of confrontation, was observed in about 35%
of pairings. Although isolates were often incompatible with
each other, all of them were eventually placed into one
group based on their compatibility with a common third
strain (bridging), confirming the data of heterokaryon
compatibility by sul mutants. sul mutants were pathogenic
to bean and strawberry and could be used as marked strains
in epidemiological studies.
992 - Effect of the Pinus strobus invasion on the
mycoflora of litter needles
O. Koukol
Department of Botany, Faculty of Science, Charles
University, Benatska 2, Praha 2, 128 43, Czech Republic. -
E-mail: o.koukol@seznam.cz
Mycoflora of pine litter and trash (needles, twigs, cones)
was studied in the National Park Czech Switzerland (Czech
Republic). A comparison of a native pine species (Pinus
sylvestris) and an invasive pine species (Pinus strobus) was
done at two localities in a well preserved P. sylvestris
forest and in a growth of P. strobus. An experiment using
litter bags with needles was arranged for a succession
study. Between years 1999 and 2002, the litter bags were
exposed in litter of both the pine species. Needles from the
litter bags were cultivated in damp chambers. Frequencies
of occurrence of recorded fungi were analysed using
multivariate analysis and analysis of variance. Sixty-three
species of ascomycetes in both teleomorph and anamorph
stage were recorded. Two species: Tympanis neopythia
Ouell. & Piroz. and Pseudocercospora deightonii Minter
were found for the first time in the Czech Republic. Most
of the recorded fungi colonised litter of both the pine
species but reached different frequencies. Results of the
analysis proved significant differences of the frequencies
between the pine species, seasons (spring and autumn) and
the localities. The following species were recorded in high
frequencies: Selenosporella curvispora G.Arnaud,
Chloridium state of Chaetosphaeria preusii W.Gams &
Hol.-Jech. and Anavirga laxa B.Sutton. However,
according to the literature they are uncommon at pine litter.
993 - Spatial distribution of fruitbodies of macrofungi
in pine forests with Vaccinium myrtillus in northern
Latvia
I. Krastina
Institute of Biology, University of Latvia, Miera str. 3, LV-
2169, Salaspils, Latvia. - E-mail:
inguna@email.lubi.edu.lv
Investigation on spatial distribution of fruitbodies of
agaricoid and boletoid fungi was carried out during 1993-
1997 in three Pinus sylvestris stands in the northern Latvia.
Vaccinium myrtillus and mosses dominated the ground
cover of forests. 25 permanent subplots, each of 2m x 20m,
were established at each forest site. Each subplot was
divided into 40 lesser microplots with size of 2m x 0,5m.
Counting of fruitbodies was performed 5-6 times per year
during August-October. A total of 92 species were found in
the plots during the 1993-1997. The annual number of
species and their productivity considerably varied from
year to year. The fruitbodies of some species appeared in
the same or in the adjoining microplots year after year. The
distribution of fungal species was patchy. Neither more
than 3-4 (usually 0-2) species were found at the same
microplot simultaneously, though a total number of
recorded species per microplot varied from 0 to 9. A
negative correlation among species was found. Fruitbodies
of some species, such as Cortinarius croceus and C.
semisanguineus, were spread rather evenly through all
subplots. Fruitbodies of some other species were localized
in a few microplots only, and their appearance often were
related to the discriminative features of these microplots-
soil disturbance, presence of decaying wood or other
substrates, as well as the type of vegetation. However, no
spatial patterns of fruitbodies distribution were found for
the major part of species.
Book of Abstracts 299