Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme I: BIODIVERSITY AND CONSERVATION Posters
Skeletocutis carneola and S. vulgaris. We report here an
additional 30 species that are new records for the
Dominican Republic: Amylosporus campbellii, Antrodia
malicola, A. sinuosa, Antrodiella hydrophila, Aurificaria
luteo-umbrina, Ceriporia purpurea, Coriolopsis floccosa,
Cyclomyces iodinus, C. tabacinus, Daedalea aethalodes,
Fomitopsis spraguei, Gloeophyllum abietinum, G.
mexicanum, Hapalopilus salmonicolor, Inonotus hispidus,
Perenniporia cf. tephropora, Phellinus callimorphus, P.
ferrugineovelutinus, P. linteus, P. merillii, P. orientalis, P.
pini, P. robustus, Rigidoporus concrescens, Skeletocutis cf.
carneogrisea, Trametes lactinea, Trichaptum perrottetii, T.
sector, Tyromyces leucomallus and T. venustus. Including
the new records from this study, there are 133 species in 46
genera of polypores known from the DR. Part of each
collection is deposited at JBSD and O.
555 - Screening for lichenicolous fungi of Usnea
D. Persoh
Lehrstuhl Pflanzensystematik, Universität Bayreuth,
Universitätsstraße 30 - NW I, D-95440 Bayreuth,
Germany. - E-mail: derek.persoh@uni-bayreuth.de
Fungi were isolated from individuals of a species of Usnea
(U. cf. subfloridana) at three sites in the European Alps,
one of which was sampled thrice. Under semi-sterile
conditions small pieces of the lichen were transferred in the
field to various culture media, torn apart and spread over
the agar plate. In the laboratory, growing hyphae were
excised and transferred to fresh media until pure cultures
were obtained. From these strains DNA was isolated and
the SSU and ITS regions of the nrDNA were sequenced.
The SSU sequences were aligned with about 1000 fungal
sequences from GenBank and a phylogenetic tree, being
presented, was calculated. A strain was assigned to a
certain taxonomic group when its sequence clustered as
ingroup in a clade consisting exclusively of taxa of the
same taxonomic group according to the current
classification. As a result, three of the 26 isolated fungi
were identified as Basidiomycetes, whereas the remaining
taxa belong to the Pezizomycotina (Ascomycetes). Of the
latter ones 17 strains could be assigned to the following
classes: Chaetothyriomycetes, Dothideomycetes,
Eurotiomycetes, Lecanoromycetes, Leotiomycetes and
Sordariomycetes and nine to the orders Chaetothyriales,
Diaporthales, Eurotiales, Lecanorales and Xylariales. Five
strains were identified at family level (Parmeliaceae,
Trichocomaceae and Valsaceae). In addition, the nearest -
yet sequenced - relatives of the isolated fungi were
specified by an ITS nrDNA blast search in GenBank.
556 - Endophytic fungi isolated from maize (Zea mays)
and their possible role for biological control
I.C. Pimentel 1 , R.A. Campos 2 , W.L. Araujo 3 , N.M. Barros 2
& J.L. Azevedo 3*
1 Universidade federal do Paraná, POBox 19001-CEP
81531-990-Curitiba-PR, Brazil. - 2 Universidade de Caxias
do Sul, POBox 1352-CEP 95001-970-Caxias do Sul-RS,
Brazil. - 3 ESALQ-Universidade de São Paulo, POBox 83-
13400-970-Piracicaba-SP, Brazil. - E-mail:
jazevedo@esalq.usp.br
Endophytic fungi were isolated from leaves and stems of
maize collected from plants growing in greenhouse and
field conditions. Samples were collected after 20 and 40
days seed germination. Nine main genera of fungi were
isolated and the most frequent genera found were
Colletotrichum, Fusarium and Acremonium. From 1728
plant fragments quantitative significative differences were
found being field plants about three times more intensly
colonized by endophytes when compared to greenhouse
plants. No significative differences were encoutered from
number and genera of fungi collected from 40 days old
plants when compared to 20 days old ones. Endophytes
from the genera Beauveria and Paecilomyces were isolated
both from fiels and greenhouse plants. The Beauveria
isolates were classified as B. bassiana but molecular
analysis showed that they differ from the main B.bassiana
strains used in Brazil for insect biological control. B.
bassiana isolates were tested against maize pest insects and
against the bovine tick Boophilus microplus. Bioassays
have shown that endophytic isolates were as effective as
comercial strains using concentrations varying from 100 to
100 millions conidia/mL, the last being the most effective
causing up to 75% mortality in comparison with the
controls. Our result support the possible biological control
role of endophytic entomopathogenic fungi as being
suggested already by other authors.
557 - New and interesting findings of macrofungi from
the islands of Andros and Naxos (Cyclades, Greece)
E. Polemis 1* , G.I. Zervakis 1 & D.M. Dimou 2
1 National Agricultural Research Foundation, Institute of
Kalamata, Lakonikis 85, 24100 Kalamata, Greece. -
2 Agricultural University of Athens, Laboratory of
Agricultural Microbiology, Iera Odos 75, 11855 Athens,
Greece. - E-mail: elipolem@otenet.gr
Reports on the biodiversity of macromycetes in islands and
coastal areas of southeastern Mediterranean are practically
non-existent. Investigation of typical biotopes of Andros
and Naxos (the two largest islands of Cyclades in the
Aegean archipelago), e.g. xerophytic heaths, sea-shore
grasslands, dunes and maquis, resulted in significant
records. The agaricoid taxa Conocybe rickenii, Crepidotus
epibryus, Lepista rickenii, Macrolepiota phaeodisca,
Marasmiellus rosellus, Marasmius anomalus var.
Book of Abstracts 169