Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
CMA plates. The author selected Mucor rouxianus
(Calmette) Wehmer as the standard host for culturing
member of Dimargaris cristalligena van Tieghem and
Circinella umbellata van Tieghem & Le Monnier for
Syncephalis nodosa van Tieghem, Dispira cornuta van
Tieghem and D. simplex Benjamin. Samples of house mice
dung were assayed for species of representatives of the
merosporangiferous Mucorales and identified as following
species, namely, Syncephalis nodosa van Tieghem,
Dimargaris cristalligena van Tieghem, Dispira cornuta
van Tieghem, D. simplex van Tieghem, Coemansia
breviramosa Linder and C. interrupta Linder. All of above
listed six species were illustrated and described as new
record to Taiwan.
644 - A comparison of Peronospora farinosa isolates
from Chenopodium album and Spinacia oleracea using
morphological and molecular characteristics
Y.J. Choi 1 , S.B Hong 2 & H.D. Shin 3*
1
Korea University, Department of Agricultural Biology,
2
Graduate School, Korea. - National Institute of
Agricultural Biotechnology, Korean Agricultural Culture
Collection, Korea. - 3 Korea University, Division of
Environmental Science and Ecological Engineering,
Korea. - E-mail: hdshin@korea.ac.kr
Downy mildews of any chenopodiaceous host are currently
treated as a single species, Peronospora farinosa.
Microscopic examination of the Korean isolates from
Chenopodium album (CAI) and Spinacia oleracea (SOI)
revealed that they are morphologically different. The
conidia are minutely papillate in CAI, but non-papillate in
SOI. The ultimate branchlets of CAI are mostly longer than
those of SOI. Sequence analyses of the internal transcribed
spacer region 1 of ribosomal DNA showed that they are
clustered into two different groups. SOI shared only ca.
91% nucleotide sequence homology with CAI, but showed
ca. 94% homology with Peronospora corydalis isolates.
On the other hand, CAI had higher sequence homology
with Peronospora destructor isolate than with SOI.
Consequently, we believe that downy mildews from C.
album and S. oleracea could be considered as separate
species.
645 - Taxonomic revision of Uromyces species on
cultivated legumes in Japan
W.H. Chung 1 , T. Tsukiboshi 2 & M. Kakishima 1*
1 Institute of Agriculture and Forestry, University of
Tsukuba, Tsukuba 305-8572, Japan. - 2 National Institute of
Agro-Environmental Sciences, Tsukuba 305-0856, Japan. -
E-mail: kaki@sakura.cc.tsukuba.ac.jp
Cultivated legumes are important crops throughout the
world. Three Uromyces species; U. appendiculatus, U.
vignae, and U. viciae-fabae, including varieties on
cultivated legumes, have been reported in Japan. Although
these species and varieties were classified mainly by
morphology of the teliospores and urediniospores, their
morphological delimitation is obscure. Therefore, the
morphological characteristics of teliospores and
urediniospores were clarified and molecular phylogenetic
analyses based on the sequences of ribosomal DNA regions
were carried out. More than 300 specimens collected from
various areas of Japan were used for morphological
observations. We examined the morphological
characteristics of teliospores and urediniospores by light
and scanning electron microscopy. Based on these
observations, specimens were classified into three groups.
About 80 specimens were selected from the specimens
used for morphological observations and sequences of LSU
rDNA (D1/D2) and ITS regions were analyzed. Specimens
were separated into two clades in phylogenetic trees by the
D1/D2 regions. However, they were separated into three
clades in phylogenetic trees by the ITS regions.
Correlations between morphological groups and
phylogenetic groups based on these results suggest a
revision of these species and varieties.
646 - Identification of Armillaria isolates from Bhutan
based on ITS and IGS-1 sequences
M.P.A. Coetzee 1* , B.D. Wingfield 1 , T. Kirisits 2 , D.B.
Chhetri 3 & M.J. Wingfield 1
1 Department of Genetics, Forestry and Agricultural
Biotechnology Institute (FABI), University of Pretoria,
Pretoria, South Africa. - 2 Institute of Forest Entomology,
Forest Pathology and Forest Protection, Universität für
Bodenkultur, Hasenauerstrasse 38, A-1190 Vienna,
Austria. - 3 Renewable Natural Resources Research Centre
(RNR-RC), Yusipang, P.O Box 212, Thimphu, Bhutan. - Email:
martin.coetzee@fabi.up.ac.za
Armillaria root rot is known to occur in conifer forests in
Bhutan. The species causing this disease have, however,
not been identified. The aim of this study was to determine
the identity of field isolates collected from Pinus
wallichiana and Abies densa at two localities in western
Bhutan. DNA sequence data were obtained for the ITS and
IGS-1 regions of the rRNA operon and compared with
those of Armillaria spp. available on GenBank and our
own database. IGS-1 sequences for isolates from P.
wallichiana had the greatest level of similarity (98%) with
sequences from the same DNA region of A. mellea subsp.
nipponica from Japan. Although ITS sequence data are not
available for A. mellea subsp. nipponica, sequences from
this DNA region were most similar to the closely related A.
mellea s.s. from Japan. Based on these findings, we believe
that the isolates from P. wallichiana included in this study,
represent A. mellea subsp. nipponica. ITS sequences for
isolates from A. densa were most similar (99%) to A.
cepistipes on GenBank. Parsimony analysis placed these
isolates in a sister group to A. cepistipes, but also indicated
that they are very closely related to A. gallica and A.
sinapina. We believe that the isolates from A. densa
represent A. cepistipes, but sexual compatibility tests are
required to confirm this view, especially because A.
cepistipes is closely related to A. gallica and A. sinapina.
Book of Abstracts 195