Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme V: CELL BIOLOGY AND PHYSIOLOGY Posters
induction was a transient increase in cytosolic free calcium
which correlated with a polar?apolar transition in hyphal
morphology. A transient increase in calcium was not
observed in this mutant when the morphological shift was
in the opposite direction. These results indicate that PKAdependent
phosphorylation may be important for
increasing cytosolic free calcium to induce a polar to apolar
shift in hyphal morphology.
1073 - Biological decolorization of textile dyes by
Funalia trogii
H. Bermek 1* , T. Deveci 2 , M. Erdogan 3 , O. Pekcan 3 & C.
Tamerler 1
1 Istanbul Technical University, Dept. of Molecular Biology
and Genetics, Maslak, Istanbul, 80626, Turkey. - 2 Istanbul
Technical University, Dept. of Environmental Engineering,
Maslak, Istanbul, 80626, Turkey. - 3 Istanbul Technical
University, Physics Department, Maslak, Istanbul, 80626,
Turkey. - E-mail: bermek@itu.edu.tr
Synthetic azo and disperse dyes are extensively used in
textile industries. A significant proportion of these dyes is
eluted in wastewaters. Biodegradation is an alternative to
physical and chemical methods used for waste dye
removal. In this study, the decolorization capability of a
white-rot fungus Funalia trogii for eleven synthetic azo or
disperse textile dyes were tested. Preliminary results
showed that the fungus was capable of removing four dyes,
Remazol blue RR, Dianix blau BRL, Dianix blau ACE and
Telon schwarz by 38.6%, 50.9%, 32.8% and 54.5%
respectively during a period of 5 days. For various
dyestuffs, mechanism of decolorization appeared to be
different. The effect of inoculum age and agitation on dye
removal was investigated for obtaining maximum
decolorization. Degree and type of dye removal was found
to be dependent on the time of dye addition to the fungal
cultures. Consequently, morphological differences in
mycelial growth were also observed depending on addition
time of dyestuffs.
1074 - Effect of oxygen in the production of manganese
peroxidase and laccase by a newly isolated white-rot
fungus Trichophyton rubrum LSK-27
H. Bermek 1* , I. Gulseren 1 , H. Jung 2 , K. Li 3 & C. Tamerler 1
1 Istanbul Technical University, Molecular Biology and
Genetics, Maslak - Istanbul 80626, Turkey. - 2 Sunchon
National University, Department of Forest Resources,
Sunchon 540-742, Korea. - 3 Oregon State University,
Department of Wood Science and Engineering, 102
Richardson Hall, Corvallis, OR97331-5751, U.S.A. - Email:
bermek@itu.edu.tr
Fungal mycelium morphology is a key concept in the
production of enzymes. Oxygen transfer is one of the most
important parameters that affect the morphology,
consequently the enzyme production. Our studies on the
morphology and ligninolytic enzyme production of a newly
isolated white rot-fungus Trichophyton rubrum LSK-27
constitute a very good example to this fact. Three
ligninolytic enzymes, manganese peroxidase (MnP), lignin
peroxidase (LiP) and laccase productions exhibited
variations accordingly. In shake flasks, 3 different working
volumes (wv: 10%, 25% and 50%) were studied, in which
the organism exhibited significantly different pellet size
and shape formation. Oxygen limited conditions (50% wv)
led to the formation of large puffy balls (radii ^20 mm),
however in 10% and 25% wv, cultures appeared similar in
terms of pellet size and shape with a slightly flattened
spheres (radii ^1 mm). Moreover, 10% cultures, exposed to
least oxygen limitation, contained some hyphal growth in
addition to the pellets. Our results clearly demonstrated that
this microorganism preferred oxygen rich conditions for
enzyme production. MnP and laccase productions were the
highest at 10% and 25% wv respectively No MnP and
laccase produced at 50% wv. LiP was not detected at all
cases.
1075 - Elemental analysis of edible mushroom brown
birch scaber stalk Leccinum scabrum (Bull. ex Fr.) S. F.
Gray growing in various sites in Poland
L. Bielawski * & J. Falandysz
Department of Environmental Chemistry and
Ecotoxicology, University of Gdansk, 18 Sobieskiego St.,
PL 80-952 Gdansk, Poland. - E-mail:
leszek29@chemik.chem.univ.gda.pl
The concentrations of 13 elements such as Al, Ba, Ca, Cu,
Cd, Fe, K, Mn, Mg, Na, Sr, Zn, and Hg were determined in
fruiting bodies of Brown Birch Scaber Stalk Leccinum
scabrum (Bull. ex Fr.) S. F. Gray collected in summer and
autumn 2000 from different sites in Poland. Fresh
mushrooms after cleanup from plants and soil were dried
for several days in room temperature and further dried in
electric oven at 40 degree C for 24 hours. The samples
(0.2-0.5 g) of dried and powdered pooled samples of
mushrooms (separately caps and stalks) were wet digested
with 6 ml of concentrated nitric acid under pressure in
closed TFM vessels in a microwave oven (CEM, Mars 5).
The PerkinElmer Optima 2000 DV ICP-OES instrument
was used for the analysis of mushrooms samples. In the
case of mercury CV-AAS technique was used. The method
was validated by analysing certified reference materials:
CTA-OTL-1 (Oriental Tobacco Leaves, Institute of
Nuclear Chemistry and Technology) and IAEA-359
(Cabbage Leaves, International Atomic Energy Agency).
There were some variations between trace elements
concentration and collecting places. Magnesium, zinc and
manganese were determined in quite similar concentrations
regardless of sampling site while in the case of other
elements some variations were noted. The essential
elements such sodium, potassium, magnesium and calcium
were found in higher concentrations than toxic mercury
and cadmium. Potassium is the most abundant amongst of
the elements quantified.
Book of Abstracts 325