Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
monographed (Ritschel 2001, Schubert 2001), based on
light and scanning electron microscopical as well as
molecular (rDNA ITS sequences) examinations, and
includes comprehensive descriptions and illustrations of 40
Fusicladium, 7 Pollaccia and 5 Spilocaea species. Venturia
and its anamorphs proved to be monophyletic. Based on a
combination of morphological and molecular examinations
and data, the generic delimitation and the species concept
of Venturia anamorphs have been re-evaluated. Features
suitable for the delimitation of species and other features
unsuitable for this purpose have been pointed out. The
structure of the conidiogenous loci (scars) and conidial
hila, which is uniform within Venturia anamorphs, proved
to be the basic characteristic for the delimitation of genera.
Morphological and molecular examinations showed that
the three anamorphic genera Fusicladium, Pollaccia and
Spilocaea are not tenable. It is suggested to merge these
genera under the well-known name Fusicladium, which is
proposed to be conserved over the older name Spilocaea.
637 - Cultural and microscopic studies of Schizophyllum
commune and Grifola frondosda (higher
basidiomycetes) strains isolated in Ukraine
A.S. Buchalo 1* , S.P. Wasser 2 & V.M. Linovitska 3
1
N. G. Kholodny Institute of Botany, National Academy of
Science of Ulraine, 2 Tereshchynkivska, Kiev, 01601,
Ukraine. - 2 International Center for Cryptogamic Plants
and Fungi, Institute of Evolution, University of Haifa,
Institute of Evolution, University of Haifa, Mt. Carmel,
Haifa 31905, Israel. -
3 NTUU 'KPI', Faculty of
Biotechnology and Biotechnique, 37 Peremogy Ave., Kiev,
03057, Ukraine. - E-mail: asbuchalo@kiev.botan.ua
The increasing interest in the cultivation of Schizophyllum
commune and Grifola frondosa depends on their
extraordinary medicinal properties. Biotechnological
application of S. commune and G. frondosa mycelial
cultures requires isolation of new strains from nature and
more detailed studies of their morphological
characteristics. Pure cultures of G. frondosa and S.
commune were isolated from fruiting bodies collected on
the territory of the Ukraine. Cultures were screened on
different agar media at the temperature interval +4 - 38 °C.
The growth rate of strains and enzyme tests on oxidases in
cultures were determined. Fast growing strains, which have
strong enzyme reactions were selected for the following
investigation. Scanning electron microscopic studies
showed that on hyphae of G. frondosa numerous clamp
connections of medallion type were present. In old parts of
colony often sprouting clamps and anastomoses are typical.
In the younger parts of a colony thin (≤ 1 mm wide)
branched hyphae (dichohyphidia) are forming. Apical and
intercallar chlamydospores are usual. In S. commune clamp
connections of a classic shape are registered. Some hypha
are densely covered with projections described by some
authors as pseudoconidia or secretory conidia. Ellipsoid
chlamydospores are often present. Teleomorph is typically
formed during 5-6 weeks. Described characteristics are
significant. For instance, clamps can be used for the control
of purity and physiologic state under cultivation.
638 - Two new hyphomycetes from Spain
M. Calduch * , J. Gené, A.M. Stchigel & J. Guarro
Rovira i Virgili University, C/ Sant Llorenç 21, 43201-
Reus, Spain. - E-mail: umb@fmcs.urv.es
Spanish mycobiota is considered highly diverse due to the
regional variety of climate and vegetation. However, the
hyphomycete check-list is still very reduced. Over recent
years, we have conducted an extensive survey of those
fungi from different habitats. Recently, we have found two
undescribed specimens belonging to Oidiodendron Robak
and Phialophora Medlar, isolated from litter and soil,
respectively. Plant debris samples were placed into moist
chambers, and soil samples were plated onto sterile Petri
dishes which were moistened thoroughly with sterile
distilled water and covered with small pieces of sterile
wood (soil bait technique). Oidiodendron myxotrichoides
sp. nov. is characterized by the presence of dark brown
reticulate sporodochia, which resemble gymnothecia
(ascomata) of Myxotrichum, and by its globose or
subglobose arthroconidia. The presence of sporodochia
constitutes a new character in Oidiodendron. Phialophora
vesiculosa sp. nov. is characterized by possessing erect and
branched conidiophores with branches ending in sterile
vesicles, and by its mono- or polyphialidic, terminal or
lateral conidiogenous cells with flared collarettes, from
which spherical, brown conidia with a protuberant basal
hilum are produced.
639 - Evolutionary relationships between aquatic
anamorphs and teleomorphs: Lemonniera
J. Campbell 1* , C.A. Shearer 1 & L. Marvanová 2
1 UIUC, Dept of Plant Biology, 265 Morrill Hall, 505 S.
Goodwin Ave, Urbana, IL, U.S.A. - 2 Masaryk University
Brno, Faculty of Science, Tvrdeho 14, 602 00 Brno, Czech
Republic. - E-mail: jcampbe2@life.uiuc.edu
Mitosporic fungi have traditionally been classified based
on their method of conidiogenesis, conidial secession, and
conidium and conidiophore morphology. We wanted to
determine whether these were important phylogenetic
characters for a group of morphologically and
developmentally similar aquatic hyphomycetes, or if this
was due to convergent evolution. Cladistic analyses were
performed on the 28S rDNA of eight species of
Lemonniera and one species of Margaritispora. These two
genera both have phialidic conidiogenesis and schizolytic
conidial secession. Lemonniera has tetraradiate conidia and
Margaritispora has unbranched conidia. Molecular
analyses demonstrate that Lemonniera species are placed in
two distinct clades: one within the Leotiomycetes; the other
within the Pleosporales, Dothideomycetes. Margaritispora
is placed with Lemonniera species within the
Leotiomycetes. This demonstrates that for this group of
species conidiogenesis, conidial secession and conidium
Book of Abstracts 193