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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Tuesday August 13th Lectures<br />

were amplified and sequenced. Phylogenetic analysis based<br />

on the 5.8S gene sequences showed that these 18 white<br />

morphotypes belonged to the Ascomycota. Further<br />

identification <strong>of</strong> the white morphotypes to lower taxonomic<br />

levels was conducted by means <strong>of</strong> sequence similarity<br />

comparison and phylogenetic analysis <strong>of</strong> both the 5.8S<br />

gene and ITS regions. The origins <strong>of</strong> WMS9 and WMS10<br />

were inferred to be the genus Lophodermium <strong>of</strong> the<br />

Rhytismataceae, and the origins <strong>of</strong> WMS11, WMS13, and<br />

WMS18 were the Rhytismataceae. WMS2, WMS3,<br />

WMS4, WMS5, and WMS6 were identified to the genus<br />

Rosellinia, WMS1 to the genus Entoleuca, and WMS14 to<br />

the genus Nemania <strong>of</strong> the Xylariaceae, and the origins <strong>of</strong><br />

WMS7, WMS8, WMS12, WMS15, WMS16, and WMS17<br />

were the Xylariaceae. The value <strong>of</strong> using DNA sequence<br />

analysis in the identification <strong>of</strong> endophytic fungi is<br />

discussed.<br />

137 - Assessing diversity <strong>of</strong> leaf-inhabiting pathogenic<br />

coelomycetes in south-east Europe<br />

T.V. Andrianova<br />

M.G. Kholodny Institute <strong>of</strong> Botany, NAS Ukraine,<br />

Tereshchenkivska, 2, Kiev 01601, Ukraine. - E-mail:<br />

tand@darwin.relc.com<br />

Leaf-inhabiting pathogenic coelomycetes are difficult to<br />

monitor in nature because <strong>of</strong> their microscopic size, large<br />

number <strong>of</strong> inadequately characterized and classified<br />

morphs. About 1100 species <strong>of</strong> these anamorphic fungi<br />

have been recorded from south-east Europe. Fungal<br />

diversity assessment in these temperate and submeridional<br />

regions allow one to predict an eventual total <strong>of</strong> around<br />

15300 micr<strong>of</strong>ungi on the 5100 known vascular plants.<br />

Some names need critical revision at species rank, other<br />

anamorphic names need reassessment in the light <strong>of</strong><br />

teleomorph names and the application <strong>of</strong> one name for the<br />

holomorph. An eventual 1500-2000 leaf-inhabiting<br />

pathogenic coelomycetes can thus be expected for southeast<br />

Europe. Main centres <strong>of</strong> biodiversity are the<br />

Carpathian mountains and Crimean peninsula. About 500<br />

leaf-inhabiting coelomycete pathogens were observed in<br />

mountain forests, subalpine meadows, forest-steppes and<br />

steppes <strong>of</strong> Crimea. The eastern Carpathian virgin broadleaf<br />

and conifer forests, and alpine plant communities are<br />

characterized by over 260 <strong>of</strong> these pathogens, but this<br />

number is not final. The species richness <strong>of</strong> leaf-inhabiting<br />

coelomycetes is based on a combination <strong>of</strong> abiotic factors<br />

and host-plants diversity. Decline <strong>of</strong> the total population <strong>of</strong><br />

these fungi, at 43% in the Carpathians, and accumulation <strong>of</strong><br />

species with agressive pathogenic characters has been<br />

observed in unstable ecosystems. Study <strong>of</strong> these fungi can<br />

provide a way to monitor stability <strong>of</strong> natural ecosystems.<br />

138 - Functional biodiversity <strong>of</strong> grassland saprotrophic<br />

fungi<br />

L.J. Deacon 1* , C.H. Robinson 1 , B.W. Bainbridge 1 & J.C.<br />

Frankland 2<br />

1 King's College London, Division <strong>of</strong> Life Sciences,<br />

Franklin-Wilkins Building, 150 Stamford Street, London<br />

SE1 9NN, U.K. - 2 Centre for Ecology and Hydrology<br />

Merlewood, Windermere Road, Grange Over Sands,<br />

Cumbria LA11 6JU, U.K. - E-mail:<br />

lewis.deacon@kcl.ac.uk<br />

The aim <strong>of</strong> this project is to characterise the functional<br />

biodiversity <strong>of</strong> saprotrophic decomposer fungi in a<br />

grassland community. The presence <strong>of</strong> all saprotrophic<br />

fungal species in a grassland community may not be<br />

necessary to maintain the function <strong>of</strong> the ecosystem, i.e.<br />

some species may be functionally redundant in the process<br />

<strong>of</strong> decomposition because several species may break down<br />

the same carbon and nitrogen substrates. Fungal isolates<br />

obtained from an upland grassland soil in Scotland, were<br />

selected to cover not only the most abundant species<br />

(common isolations), but also a cross section <strong>of</strong> the fungal<br />

community (occasional isolations) from different treatment<br />

plots (control, lime, nitrogen and nitrogen plus lime) at the<br />

field site. Isolates were tested for their ability to grow on<br />

specific substrates, in defined and semi-defined media.<br />

Primarily the activity <strong>of</strong> cellulolytic enzymes, ligninolytic<br />

enzymes, pectinase, amylase and chitinase were tested on<br />

solid media. Twelve isolates were chosen for further<br />

screening for the utilisation <strong>of</strong> smaller molecular weight<br />

carbon and nitrogen sources using the BIOLOG system.<br />

Further work is in development to test the paired isolates<br />

ability to utilise and compete for natural substrates<br />

available in the grassland.<br />

139 - Guatemalan Macr<strong>of</strong>ungi: diversity and uses<br />

R.E. Flores 1* , M.C. Bran 1 , O. Morales 1 & M. Honrubia 2<br />

1 Universidad de San Carlos, DIGI/Facultad CCQQ y<br />

Farmacia. Edif T-12 Ciudad Universitaria 01012,<br />

Guatemala. - 2 Universidad de Murcia, Depto. Biología<br />

Vegetal (Botánica). Campus de Espinardo 30100, Spain. -<br />

E-mail: rfloresa@yahoo.com<br />

Many fungal species from North to South America and<br />

vice versa found place in Guatemala due to its geographical<br />

position and orographic relief. Many <strong>of</strong> them are<br />

mycorrhizal with pines, oaks, alders and fir. In addition,<br />

there is also a large diversity <strong>of</strong> saprophitic species,<br />

especially in the tropical areas. Mayan people in Guatemala<br />

have used mushrooms as part <strong>of</strong> their rituals and as a<br />

source <strong>of</strong> food since many centuries ago. Currently<br />

mushrooms are used as food and as medicine but<br />

exceptional uses as insecticide or decorative elements are<br />

reported. The main researches on macr<strong>of</strong>ungi in Guatemala<br />

have been directed for 10 years ago by the University <strong>of</strong><br />

San Carlos. Currently its <strong>Mycological</strong> Herbarium contains<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong> 45

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