Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
differences in Nei's coefficient of genetic differentiation
derived from universally primed PCR (UP-PCR)
fingerprints it was possible to recognize distinctions among
the three nomengroups and B. cinerea and B. squamosa.
Primers, designed from a sequence characterized UP-PCR
fragment, were used for direct sequencing of DNA from
isolates of the 16 chromosome nomengroups. Because of
apparent ambiguities in the UP-PCR fragment from the 32chromosome
group, it was cloned and sequenced.
Clustering show identity with the small-spored B. aclada
and the large-spored B byssoidea for the two cloned
molecules from B. allii. Further, the internally transcribed
spacer rDNA (ITS) amplicons of B. aclada has 2 SphI
restriction sites; that of B. allii has 1 SphI site. The
cumulative data suggest that the three groups are
genetically distinct from each other and that isolates B.
aclada and B. byssoidea were the ancestors of B. allii.
793 - Morphological and molecular characterization of
small-spored Alternaria species
S.H. Yu * & B.R Kim
Chungnam National University, College of Agriculture,
220 Gung-dong, Yuseung-gu, Daejeon 305-764, Korea. -
E-mail: shunyu@cnu.ac.kr
The importance and diversity of the genus Alternaria
highlights the need for accurate identification of species.
However, many small-spored Alternaria isolates have been
misidentified due to the use of spore size as the only
identifying character. In this study eighty one isolates of
small-spored Alternaria were segregated into
morphological and pathogenic groups or species as A.
gaisen, A. mali, A. tenuissima, A. longipes, A. citri, A.
arborescens, A. infectoria, and A. alternata. Molecular
characteristics of these isolates were determined using
universal rice primer (URP)-PCR analysis, sequence
analyses of nuclear internal transcribed spacer (ITS) and
mitochondrial small subunit (SSU) ribosomal DNA
(rDNA). Based on cluster analysis of URP-PCR fragment
patterns, the eighty one isolates were segregated into
distinct groups that are morphologically similar but
identifiable as A. gaisen, A. mali, A. tenuissima, A.
longipes, A. citri, A. arborescens, A. infectoria, and A.
alternata. Based on analyses of ITS and mitochondrial
SSU rDNA sequence data, there was no variability in ITS
and mt SSU rDNA sequences for species among A. gaisen,
A. mali, A. tenuissima, A. longipes, A. citri, A. arborescens,
and A. alternata. However, A. infectoria was differ from
other species at 21 and 16 nucleotides in ITS and mt SSU
rDNA, respectively.
794 - A contribution to the identification of
Trichoderma species in Iran
- Zafari 1* , - Ershad 2 , - Zare 2 & - Alizadeh 3
1 Bu Ali Sina University and Tarbiat Modarres University,
Bu Ali Sina University Hamadan, Iran. - 2 Plant Pestes and
Diseases Research Institute, Tehran, Iran. - 3 Tarbiat
Modarres University, Tehran, Iran. - E-mail:
zafari_d@yahoo.com
The aim of the present study is to identify Trichoderma
species isolated from Iran. Soil samples were collected
from different parts of Iran focusing on the agricultural
fields. Trichoderma selective media and malt extract agar
(MEA) were used to isolate Trichoderma species from the
soil samples and Petri plates were incubated at 25 °C in the
dark for the first 24 hours and then 12/12 (dark/ flourescent
light) with the same temperature. All the cultures were
purified on 2% water agar by hyphal tip method prior to
morphological examination. Morphological observations
were carried out on the cultures grown on 2% MEA at 20
°C under ambient laboratory conditions. Microscopic
features of conidiophores and shape and size of conidia
were studied and recorded 3-5 days after inoculation. Out
of 350 obtained isolates, using morphological features ten
species (T. citrinoviride, T. longibrachiatum, T.
saturnisporum, T. hamatum, T. harzianum, T. inhamatum,
T. tomentosum, T. virens, T. asperellum, T. koningii) were
identified. Among the species T. harzianum with 150 and
T. virens with 57 isolates were the most frequent species.
795 - An integrated approach to the taxonomy of plantassociated
Verticillium species
R. Zare
Plant Pests & Diseases Research Institute, P.O. Box 1454,
Tehran 19395, Tehran, Iran. - E-mail:
simplicillium@yahoo.com
Molecular approaches were used to re-evaluate the
morphological criteria used to identify plant-associated
species of Verticillium Nees. ITS-RFLPs divided the 31
studied strains of seven Verticillium species, (including the
type species) into four clusters. Cluster one comprised
strains of the type species, V. luteo-album (Link: Fries)
Subramanian, cluster two V. albo-atrum Reinke &
Berthold, V. dahliae Klebahn, V. nubilum Pethybridge and
V. tricorpus Isaac; cluster three comprised strains of V.
theobromae (Turconi) E. Mason & S. Hughes, and cluster
four comprised strains of V. nigrescens Pethybridge. Betatubulin
gene RFLPs offered a higher degree of resolution,
distinguishing all seven species from each other. The
highest degree of resolution was obtained from
mitochondrial DNA-RFLPs that divided strains of V.
theobromae and V. nigrescens into infraspecific groups.
The beta-tubulin gene digested by Hae III offers a reliable
way to separate the two economically important and
controversial species V. albo-atrum and V. dahliae.
Book of Abstracts 239