Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme V: CELL BIOLOGY AND PHYSIOLOGY Posters
cooled and non-cooled mycelium P. ostreatus on isolated
plant mitochondria (T. aestivum L.) was observed
uncoupling of oxidative phosphorilation. Uncoupling of
mitochondria oxidation and phosphorilation processes
contribute to heat generation and reduce oxidative stress
that may be important to survival of mycelium. The results
acquired confirm a certain degree of similarity in
mechanisms of plant and fungi cells protection at lowtemperature
stress. However, unlike plants, mycelium of
the fungi of boreal zone is able to survive at low negative
temperatures without preliminary acclimation, and contains
protection proteins in the conditions of 'comfortable'
temperature.
1203 - Alpha-galactosidase gene cloning from
Aspergillus fumigatus
B. Yalcindag 1* , Z.B. Ogel 1 , U. Bakir 2 & P. Biely 3
1 Middle East Technical University, Department of Food
Engineering, 06531, Ankara, Turkey. - 2 Middle East
Technical University, Department of Chemical
Engineering, 06531, Ankara, Turkey. - 3 Slovak Academy of
Sciences, Institute of Chemistry, SK-84 238, Bratislava,
Slovakia. - E-mail: banuy@metu.edu.tr
In this study, molecular cloning studies were performed on
the α-galactosidase gene of Aspergillus fumigatus IMI
385708. Degenerate primers were designed based on amino
acid sequence of the purified enzyme and conserved
sequences among α-galactosidases of Aspergillus and
Penicillium species. These primers were used in PCR to
amplify α-galactosidase gene fragments. A 900 bp PCR
product was selected as a probe for further studies. RNA
dot blot analysis showed that the highest expression of αgalactosidase
mRNA was on the fifth day of growth on
locust bean gum. This information was employed in cDNA
synthesis. The synthesized cDNA was ligated onto Lambda
ZAP Express vector to construct the primary cDNA library.
After amplification of the primary library, screening
studies were performed. At the end of primary and
secondary screening, a number of putative positive plaques
were obtained for further characterization studies.
1204 - Transformation of Neotyphodium lolii with
plasmids containing a native promoter disturbs the
symbiotic interaction with its host
N. Zhang 1 , V. Scott 1 , T.H. Al-Samarrai 1 , Y.Y. Tan 1 , M.J.
Spiering 1 , L. McMillan 1 , D.B. Scott 1 , M.J. Christensen 2 &
J. Schmid 1*
1 IMBS, Massey University, Palmerston North, New
Zealand. - 2 2AgResearch Grasslands, Palmerston North,
New Zealand. - E-mail: J.Schmid@massey.ac.nz
Neotyphodium lolii is an endosymbiont of perennial
ryegrass. It produces alkaloids neurotoxic to herbivores.
We wanted to determine in planta expression patterns of
the N.lolii 3-hydroxy-3-methylglutaryl-CoA reductase
(HMG CoA reductase) gene, believed to be involved in
neurotoxin synthesis. We transformed the endophyte with
plasmids, in which DNA fragments upstream of the open
reading frame of the HMG CoA reductase gene controlled
expression of the GUS reporter gene. In laboratory
cultures, >400 bp were required for expression and >=1100
bp for maximum expression. Transformants often showed
abnormal hyphal morphology when growing within
ryegrass. This occurred significantly more often in
transformants which carried additional functional HMG
CoA reductase promoter sequences compared to
transformants with HMG CoA reductase gene upstream
sequences of