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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme I: BIODIVERSITY AND CONSERVATION Posters<br />

recorded at single visit (H. reidii, H. marchii, H.<br />

subpapillata, together with some gasteromycetes and rare<br />

Clavariaceae. LPA Horná Orava - wet meadows and<br />

peatbogs with species as H. calyptraeformis, H.<br />

substrangulata, H. coccineocrenata, H. turunda a.o.<br />

National Park (NP) Slovenský Raj - seminatural grasslands<br />

<strong>of</strong> European importance with very high biodiversity.<br />

<strong>Mycological</strong> research has just started, but species as H.<br />

nitrata, H. marchii, H. perzistens and H. substrangulata<br />

were recorded.<br />

528 - Building <strong>of</strong> the central database <strong>of</strong> biological<br />

collections in the museums <strong>of</strong> the Slovak Republic<br />

I. Kautmanova * , J. Uhlirova & J. Kautman<br />

Slovak National Museum - Nature Historical Museum,<br />

Vajanského nábr.2, 821 05 Bratislava, Slovakia. - E-mail:<br />

botanika@snm.sk<br />

In 2001 the project <strong>of</strong> building national database <strong>of</strong><br />

biological collections, in which 31 Slovak museum take<br />

part, has started. Guarantee is the Nature Historical<br />

Museum (NHM) <strong>of</strong> the Slovak National Museum and the<br />

project is aimed to the identification and documentation <strong>of</strong><br />

chosen components <strong>of</strong> the Slovak flora and fauna, with the<br />

accent on the rare and endangered species. At present the<br />

structure <strong>of</strong> database program is prepared and in 2003 first<br />

data will be filled in. Central node <strong>of</strong> the database will be at<br />

the NHM and in 2005 first outputs will be presented on the<br />

web. Herbary collections preserved in 25 museums contain<br />

721 405 specimens. Fungi (including lichens) are 157 936<br />

specimens. Main mycological collections are at: Slovak<br />

National Museum-Nature Historical Museum, Bratislava<br />

(BRA) - 146 877, Museum <strong>of</strong> the Tatra National Park<br />

(TNP) - 5 348, Forestry and Timber Museum, Zvolen - 1<br />

910, Museum Nitra (NIM) - 1139, Central-Slovakian<br />

Museum, Banská Bystrica (SMBB) - 491, Povazie<br />

Museum, Zilina - 463, Orava Museum <strong>of</strong> P.O.Hviezdoslav,<br />

Dolný Kubín - 415, Slovak Museum <strong>of</strong> Nature Protection<br />

and Speleology, Liptovský Mikulás (MOP) - 334, Saris<br />

Museum, Bardejov (SMB) - 200 specimens.<br />

529 - Role <strong>of</strong> mobile introns in diversity <strong>of</strong> mtDNAs <strong>of</strong><br />

imperfect black Aspergilli<br />

F. Kevei * , Á. Juhász & Zs. Hamari<br />

Department <strong>of</strong> Microbiology, Faculty <strong>of</strong> Sciences,<br />

Univesity <strong>of</strong> Szeged, H-6701 SZEGED, P.Ö.Box 533,<br />

Hungary. - E-mail: kevei@bio.u-szeged.hu<br />

A high level <strong>of</strong> intraspecific mtDNA variability was<br />

reported earlier among black Aspergilli, indicated by<br />

various RFLP pr<strong>of</strong>iles <strong>of</strong> isolates <strong>of</strong> Aspergillus niger, A.<br />

tubingensis, A. carbonarius and A. japonicus. To study the<br />

reason for the mtDNA polymorphism physical- and<br />

functional maps <strong>of</strong> mtDNAs representing different<br />

RFLPtypes were constructed than the variable regions<br />

cloned and sequenced. These revealed that apart from<br />

minor nucleotide changes, the presence <strong>of</strong> introns at<br />

different positions and their appearance in various numbers<br />

in the mtDNAs are responsible for the mitochondrial<br />

genome diversity and size differences. The reasons <strong>of</strong><br />

variability between two mtDNA types <strong>of</strong> A. carbonarius<br />

can be attributed to the presence or absence <strong>of</strong> a 1.1 kb<br />

group I intron in their cox2 subunit. Sequence comparison<br />

<strong>of</strong> two mtDNA RFLP types <strong>of</strong> A. japonicus revealed that<br />

they differed from each other in at least two group I introns<br />

in cox1 subunit and one group I intron in cob gene.<br />

Organisation <strong>of</strong> four A. niger mtDNA types proved to be<br />

very similar, but one <strong>of</strong> the three observed variable regions<br />

included a group I intron in the cox1 subunit was present in<br />

three different forms. The mobile introns (bearing ORFs)<br />

play an important role with their homing process<br />

generating recombinant mtDNAs in the interaction among<br />

mitochondrial genomes after mitochondrial transmission.<br />

The homing endonucleases activate a double strand break<br />

that is finally repaired by a repaire mechanism.<br />

530 - Arbuscular mycorrhizal status <strong>of</strong> plant species<br />

from Western Ghats <strong>of</strong> Goa, India<br />

S. Khade * & B. Rodrigues<br />

Department <strong>of</strong> Botany, Goa University, India. - E-mail:<br />

shardakhade@rediffmail.com<br />

Western Ghats are one <strong>of</strong> the hot spots <strong>of</strong> biodiversity in<br />

the world and the state <strong>of</strong> Goa lies in the heart <strong>of</strong> Western<br />

Ghats. In the present paper, AM fungal status <strong>of</strong> 62 plant<br />

species, viz. pteridophytes, herbs, shrubs, trees and plants<br />

with under storage ground organs from Western Ghats <strong>of</strong><br />

Goa have been discussed. All the plant species selected for<br />

study, exhibited the presence <strong>of</strong> AM fungal association.<br />

The average root colonization ranged from 30%<br />

(pteridophytes) to 70% (shrubs). Whereas the average<br />

spore density <strong>of</strong> AM fungi ranged from 52 spores 100 -1 g<br />

soil (pteridophytes) to 528 spores 100 -1 g soil (plants with<br />

under ground storage organs). Species composition <strong>of</strong> AM<br />

fungi revealed the presence <strong>of</strong> five genera viz.,<br />

Acaulospora (9), Glomus (19), Gigiaspora (3),<br />

Scutellospora (5) and Sclerocystis (5) with species number<br />

given in parenthesis. A total <strong>of</strong> 41 species <strong>of</strong> AM fungi<br />

were recovered during the study. Scutellospora gregaria,<br />

Glomus fasiculatum and Sclerocystis taiwanensis were the<br />

most frequently occurring AM fungal species. The present<br />

paper is first report on AM fungal association in plant<br />

species from Western Ghats <strong>of</strong> Goa and contributes data<br />

necessary for further studies on AM fungi from this region<br />

<strong>of</strong> which very little has been explored so far.<br />

531 - Field identification <strong>of</strong> marne yeasts using DNA<br />

hybridization macroarrays<br />

T. Kiesling, M. Diaz, A. Statzell-Tallman & J.W. Fell *<br />

University <strong>of</strong> Miami, 4600 Rickenbacker Causeway, Key<br />

Biscayne, Fl, U.S.A. - E-mail: jfell@rsmas.miami.edu<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong> 161

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