Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme III: PATHOGENS AND NUISANCES, FOOD AND MEDICINE Posters
eluted from the gel, reamplified and the fragments were
sequenced. Based on these results SCAR-PCR primers
(sequence characterized amplified region) were
constructed, the PCRs were optimized and tested against
DNA of different fungal strains. The results show that this
approach is valuable for the rapid early detection of
potential ochratoxinogenic fungi in food.
886 - Identification of Malassezia species in patients
with pityriasis versicolor submitted to the Razi Hospital
in Tehran
M. Shams 1* , M. Rasaee 2 , M. Moosavi 1 & M. Razzaghi
abyaneh 3
1 Dept.of Mycology, Faculty of Medical Sciences,Tarbiat
Modares University, P.O.Box:14115-111, Tehran, Iran. -
2 Dept.of Biotechnology, Tarbiat Modares University,
P.O.Box:14115-111, Tehran, Iran. - 3 Dept.of Myclogy,
Pasteur Institute of Iran, P.O.Box:13164, Tehran, Iran. -
E-mail: shamsm@modares.ac.ir
Lipophilic yeasts of the genus Malassezia (Pityrosporum)
belong to the normal flora of human skin and many warmblooded
animals. These fungi can produce a diverse range
of diseases that most frequent of them is pityriasis
versicolor, a chronic recurrent skin disease occurring
primarily in tropical regions. The genus Malassezia has
been recently enlarged to include seven distinct species.
This survey was undertaken to present a practical approach
for differantiation of all Malassezia yeasts isolated from
clinical materials of patients with pityriasis versicolor for
the first time in Iran. The presence of disease was
confirmed on the basis of the observation of budding yeast
cells and short curved hyphae in skin specimens by direct
microscopy. Malassezia yeasts were isolated after culturing
the samples on modified Dixon (mDixon) agar. A
combination of different characteristics includes yeast cell
morphology, ability to growth on sabouraud dextrose agar,
catalase test and ability to utilize individual Tweens
(20,40,60,80) were used for identification of species. In
general, 138 patients with pityriasis versicolor includes
52.2% male and 47.8% female were identified. Direct
microscopy and culture results were positive in 94.4% and
63% of patients, respectively. Totally, 91 isolates of
Malassezia belonging to four different species, M. globosa
(66 isolates), M. furfur (18 isolates), M. obtusa (5 isolates)
and M. sympodialis (2 isolates) were identified.
887 - Allergic fungal sinusitis (AFS) among the
candidates of functional endoscopy sinus surgery
(FESS)
T. Shokohi 1* & M. Maddani 2
1 Department of Medical Mycology and Parasitology
Mazandaran University of Medical Sciences, po box 483,
Iran. - 2 Ear, Nose,and Throat, Mazandaran University of
medical Sciences, po box 483, Iran. - E-mail:
taherehshokohi@yahoo.com
This study was designed to determine the rate of AFS
among the patients with chronic sinusitis who failed to
respond to repeated courses of antibiotic and were
candidated for FESS in Sari. Allergic mucin and Sinus
lavage were collected during FESS from 100 Patients
meeting diagnostic criteria for AFS and were submitted for
mycology and pathology investigations. The specimens
were centrifuged and the sediment was mounted in KOH
and Gram stained for direct examination. Multiple
fragments of mucosa were removed at surgery and stained
with H&E and PAS for the pathology evaluation. In this
study we report 9 proven cases of AFS (with demonstration
of fungal hyphae by direct exam and culture) and 8
suspected cases (confirmed by direct exam or culture). The
patients' age ranged 12 to 62 years with a mean age of 24.5
years with female predominance. All of the patients were
immunocompetent. 47% of the patients had a history of
atopy. Histopathologically, hyphae were not seen. The
genera of the fungus identifiable in all but in one patient it
was not identifiable. 53% of isolated fungus are of hyaline
hyphomycete particularly Aspergillus and Penicillium and
47% of them are members of dematiaceous family
particularly Cladosporium and Nigrospora. In eleven out
of the seventeen, fungal hyphae were noted and in all of the
seventeen, there were positive fungal culture. During 6
months follow up; we identified recurrence in 2 patients
clinically without any microscopy and culture evidence.
888 - Identification of virulence variety in ascochyta
blight fungus [Ascochyta rabiei (Pass) Lab.] using
differentials lines
F. Shokoohifar 1 , A. Bagheri 2 & M.F. Rastegar 2*
1 Research Center For Plant Science, 2 Sardadvar av.;
Bahar St.; Zip Code:91747484815; Mashhad., Iran. -
2 Ferdowsi University of Mashhad, College of Agriculture,
P.O.Box;91775-1163; Mashhad., Iran.
The study was conducted to distinguish the pathogenecity
variety of the fungus population in the country. To achieve
this goal, 26 isolates were choosing as candidates for 16
provinces that were the most important chickpea
production areas. Pathogenecity test was achieved using of
16 differential lines in the greenhouse condition. It was
used current methods for classification of isolates as well
as a new method on the base of analysis of variance. The
later method was better than other methods in classification
of variety because of statistical base. In this method
differential lines have been grouped in six levels of
resistance and isolates have been grouped in six
pathotypes. Also their distribution pattern has been
identified in Iran. Results of this method are more useful
for resistance selecting in national mass. As whole, the
study revealed that there were too vigorous pathogenic
group in west and north west of the country, that none of
tested lines didn't show any resistance an this study. It
seems that it is better to design breeding projects for
screening of resistance line regarding to identify pathotype
groups in each region.
Book of Abstracts 267