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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme IV: POPULATION DYNAMICS AND ECOLOGY Posters<br />

1049 - Survival <strong>of</strong> insect target and biocontrol fungus in<br />

a greenery plantation<br />

S. Vestergaard * , C. Nielsen, S. Harding & J. Eilenberg<br />

Royal Veterinary and Agricultural University,<br />

Thorvaldsensvej 40, 1871 Frb. C, Denmark. - E-mail:<br />

sve@kvl.dk<br />

The insect family Curculionidae contains some <strong>of</strong> the<br />

economically most important pest insect species in<br />

greenery production for Christmas decorations in Denmark.<br />

In particular, the two species Strophosoma<br />

melanogrammum and S. capitatum are important pest<br />

species. At present no chemical treatment <strong>of</strong> these weevils<br />

is allowed in Denmark. Biological control including the use<br />

<strong>of</strong> insect pathogenic fungi from Hyphomycetes may thus<br />

provide a potential to minimise the damage caused by these<br />

weevils. Isolates <strong>of</strong> the insect pathogenic fungi<br />

Metarhizium anisopliae Beauveria bassiana and<br />

Paecilomyces spp. were studied in the laboratory and field.<br />

In the laboratory, all tested isolates were able to infect and<br />

cause mycosis in both adults and larvae <strong>of</strong> S.<br />

melanogrammum and S. capitatum. Based on the results<br />

from the laboratory bioassays, isolates were chosen for<br />

release under field conditions in an Abies procera<br />

plantation, where different application strategies were used.<br />

Spatial-temporal distribution <strong>of</strong> the released fungus in the<br />

soil was monitored by cfu per g soil. Weekly sampling<br />

from emergence traps monitored survival <strong>of</strong> adult weevils.<br />

Adults were collected, counted and determined to species<br />

level and incubated individually in plastic cups to estimate<br />

the prevalence <strong>of</strong> the applied fungus as well as the<br />

prevalence <strong>of</strong> natural infections. Preliminary results proved<br />

that populations <strong>of</strong> weevils in the treated plots were<br />

reduced significantly.<br />

1050 - Do Piceirhiza bicolorata mycobionts form both<br />

ecto- and ericoid mycorrhizas?<br />

L. Villarreal-Ruiz 1* , I. Anderson 2 & I.J. Alexander 1<br />

1 Department <strong>of</strong> Plant and Soil Science, University <strong>of</strong><br />

Aberdeen, Cruickshank Building St Machar Drive,<br />

Aberdeen, AB24 3UU, Scotland, U.K. - 2 Soil Quality &<br />

Protection, The Macaulay Institute, Craigiebuckler,<br />

Aberdeen, AB15 8QH, Scotland, U.K. - E-mail:<br />

l.virl@abdn.ac.uk<br />

Ectomycorrhizas <strong>of</strong> the Piceirhiza bicolorata morphotype<br />

were found commonly associated with Pinus sylvestris<br />

seedlings and mature trees in Glen Tanar Native Pinewood,<br />

Northeast Scotland. Fungal isolates from P. bicolorata<br />

mycorrhizas are thought to be part <strong>of</strong> the Hymenoscyphus<br />

ericae aggregate, and this raises the possibility that they<br />

form mycorrhizas with both the pine trees and the<br />

understorey ericoid vegetation. In this study, strains from<br />

P. bicolorata were isolated from soil cores collected in a<br />

160 year-old natural stand. The mycobiont was cultured<br />

and identified by extracting genomic DNA and sequencing<br />

the PCR- amplified ITS regions. Phylogenetic analysis<br />

showed that our isolate grouped in clade 4 <strong>of</strong> Vrålstad et al.<br />

(New Phytol., 2002) along with many other isolates from P.<br />

bicolorata-type ectomycorrhizas, including Phialophora<br />

finlandia. Mycorrhizal synthesis were performed to test the<br />

ability <strong>of</strong> our isolate to form ECM and ERM on P.<br />

sylvestris seedlings and ericoid plants respectively, and to<br />

cross infect between P. sylvestris + P. bicolorata<br />

(previously synthesized) and Vaccinium myrtillus. The<br />

synthesis with Scots Pine seedlings produced black<br />

morphotypes with or without hyaline tips and with<br />

emanating hyphae. Pure culture synthesis with V. myrtillus<br />

seedlings produced typical ERM: these were also produced<br />

in the cross infection experiments. In both cases the growth<br />

<strong>of</strong> Vaccinium plants forming ERM was stimulated relative<br />

to the growth <strong>of</strong> uninfected plants.<br />

1051 - Strategies for sustainable land management:<br />

quarry recovered through truffle-culture<br />

M. Vinay * , L. Baciarelli Falini & M. Bencivenga<br />

Dip. Biologia vegetale e biotecnologie agroambientali-<br />

Università di Perugia, Borgo xx giugno 74 06121 Perugia,<br />

Italy. - E-mail: mavinay@tin.it<br />

Building materials come from quarries. The opening <strong>of</strong> a<br />

quarry is a geological damage and it causes the complete<br />

destruction <strong>of</strong> vegetation. Italian laws provide for each<br />

open quarry project an environmental recovery and<br />

steadying plan through soil-modelling and vegetationplanting<br />

in order to repair the damage and avoid<br />

subsequent trouble. A concrete case <strong>of</strong> quarry recovery<br />

through truffle-cultivation is analysed.<br />

1052 - The identification and frequency <strong>of</strong> occurrence<br />

<strong>of</strong> higher filamentous marine fungi on mangroves in a<br />

south Florida mangle<br />

C. Vogel 1* , S. Schatz 2 , H. Laubach 3 & A. Rogerson 1<br />

1 Nova Southeastern University, College <strong>of</strong> Oceanography,<br />

8000 North Ocean Drive, Dania FL 330044, U.S.A. -<br />

2 Nova Southeastern University, Colleges <strong>of</strong> Optometry and<br />

Oceanography, Davie FL 33314, U.S.A. -<br />

3 Nova<br />

Southeastern University, College <strong>of</strong> Medical Sciences,<br />

Davie FL 33314, U.S.A. - E-mail: vogelc@nova.edu<br />

Little is known regarding the occurrence and distribution <strong>of</strong><br />

the higher filamentous fungi on mangroves in South<br />

Florida, USA. Previous studies have demonstrated that<br />

marine fungi are an important degradative component and<br />

assume an important role in nutrient recycling systems in<br />

estuarine and near-shore ecosystems. The purpose <strong>of</strong> this<br />

study was to collect, identify and prepare a key to the<br />

higher filamentous fungi occurring in the mangle <strong>of</strong> J. U.<br />

Lloyd State Park on the southeast coast <strong>of</strong> Florida, USA.<br />

Over a period <strong>of</strong> ten months 33 species were identified<br />

including 1 Basidiomycete, 8 Deuteromycetes, and 24<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong> 317

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