Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
745 - Characterization of Laetiporus sulphureus
complex in Japan using phylogenetic analyses,
morphological study and incompatibility tests
Y. Ota * & T. Hattori
Forestry and Forest Products Research Institute, Tsukuba,
305-8687, Japan. - E-mail: yuota@ffpri.affrc.go.jp
Phylogenetic analyses, morphological studies and
incompatibility tests were conducted to define intra-generic
taxa of Japanese Laetiporus sulphureus complex.
Phylogenetic analyses based on the nucleotide sequences of
the ITS regions of the ribosomal DNA and beta-tubulin
gene suggest that Japanese Laetiporus spp. are divided into
four distinct groups (groups A-D). European population of
L. sulphureus is clearly distinct from all Japanese groups.
Each group shares similar morphological characteristics
and host range (conifers vs. hardwoods). Group A has a
white pore layer, pinkish orange pileus surface, and
imbricated pilei. This group is distributed in the cooltemperate
area and associated with hardwoods. Group B
has lemon yellow pore layer, pinkish orange pileus surface,
and imbricated pilei. This group is associated with conifers.
Group C has a lemon yellow pore layer, yellow pileus
surface and non-imbricated pilei. Sometimes it produces
semi-globose basidiocarps with abundant chlamydospores
in the context. Group C is distributed in the warmtemperate
area of central parts of Japan. Group D has a
lemon yellow pore layer and pinkish orange to yellow
pileus surface. This group also produces semi-globose
form. This group is distributed in the warm-temperate to
subtropical areas of southern part of Japan. Single spore
isolates from groups A, B and C were incompatible each
other, which revealed that L. sulphureus complex in Japan
is composed of at least three species.
746 - Penicillium tulipae and P. carotae, two new species
belonging to the series Corymbifera
D.P. Overy * & J.C. Frisvad
Mycology Group, Bio-Centrum, DTU, Building 221,
Danish Technical University, DK-2800, Kgs. Lyngby,
Denmark. - E-mail: do@biocentrum.dtu.dk
Taxa of the Penicillium series Corymbifera are known for
their synnemata and association with the rhizosphere of
vegetables, most commonly with bulbs. The known species
P. allii, causing a storage rot of garlic, P. hordei, associated
with cereals, and P. hirsutum associated with a variety of
vegetables can easily be separated based on colony
morphology. The concepts of P. albocoremium and P.
venetum, reported to cause a severe bulb rot on a variety of
commercially grown flower and Allium species, later
evolved from P. hirustum senso lato as a response to
apparent differences in secondary metabolism. Further
secondary metabolite investigations involving P.
albocoremium indicated the potential presence of two or
more taxa. Described here using micromorphology, colony
characteristics on various media and chemotaxonomic
profiling are P. albocoremium sensu stricto and two new
species, P. tulipae and P. carotae, which were resolved
during a taxonomic survey of P. albocoremium isolates
contained within the IBT culture collection. Although these
novel taxa are micromorphologically quite similar, their
unique secondary metabolite profiles individually
distinguish them from isolates of P. albocoremium.
Moreover, these bulb associated species produce the
following mycotoxins: citrinin, penicillic acid and terrestric
acid by P. carotae and penitrem A and terrestric acid by P.
tulipae.
747 - Cladistic biogeography of Lignincola laevis
(Halosphaeriales, Ascomycota)
K.L. Pang 1* , E.B.G. Jones 2 , L.L.P. Vrijmoed 3 & R.Y.C.
Kong 3
1 School of Biological Sciences, University of Portsmouth,
King Henry Building, King Henry I Street, Portsmouth
PO1 2DY, England, U.K. - 2 BIOTEC, National Centre for
Genetic Engineering and Biotechnology, 113, Thailand
Science Park, Paholyothin Road, Klong 1, Klong Luang,
Pathumthani, 12120, Thailand. - 3 Department of Biology
and Chemistry, City University of Hong Kong, 83 Tat Chee
Avenue, Kowloon Tong, Hong Kong, China. - E-mail: kalai.pang@port.ac.uk
Partial large subunit and the ITS regions of the ribosomal
DNA of ten Lignincola laevis strains, which were isolated
from different geographical locations, substrata and habitat,
were sequenced and subjected to phylogenetic analysis to
determine their genetic difference. These strains do not
form distinct geographical groupings. No correlation was
discovered between strains and habitat as freshwater and
marine water isolates of L. laevis form a coherent and wellsupported
group. Strains isolated from mangroves form a
robust clade while isolates from other substrata constitute
others. Different genes infer different phylogeny for two L.
laevis strains. These results suggest that ascospores (asci)
of L. laevis may be able to disperse over long distances.
More strains from wider geographical locations, different
habitats (freshwater, brackish water and marine water) and
different substrata should be explored to explain the current
distribution pattern and evolution of aquatic fungi.
748 - Phylogenetic relationships of Hydnodon Banker
V. Patiño-Conde 1* , D. González 2 , J. Cifuentes 1 , M.
Villegas 1 & R. García-Sandoval 1
1 FCME Herbario, UNAM., PO Box 70-399, Cd.
Universitaria, CP 04510, DF., Mexico. - 2 Sistemática
Vegetal, Instituto de Ecología AC, PO Box 63, Xalapa, CP
91000, Veracruz, Mexico. - E-mail:
viole_pconde@correo.unam.mx
Book of Abstracts 225