Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
781 - Morphological variation of spore ornamentation
within the genus Gomphus
M. Villegas * & J. Cifuentes
Fac. de Ciencias, UNAM, A.P. 70-399 Cuidad
Universitaria, Del. Coyoacan. C.P. 04510, México, D.F.,
Mexico. - E-mail: mvr@hp.fciencias.unam.mx
Traditionaly the morphology of the spores has been an
important source of characters to classify species in
Gomphus. Neverthless, the understanding of morphological
variation patterns in spore ornamentation within species is
misleading. The goal of this research was to evaluate
ornamentation similarity among differents species
described as Gomphus and, to identify discrete states to use
them in systematics. We analyzed the ornamentation in
sixteen taxa using scanning electron microscopy (SEM).
Three differents patterns of ornamentation shape and two
of ornamentation distribution were differentiated.
Reticulate-lacunose ornamentation was only found in G.
retisporus, verrucose ornamentation was pretty common
and it was found in G. clavatus, G. floccosus, and G.
subclavaeformis; various degrees of verrucae fusion were
observed. Ornamentation distribution is very characteristic
in G. grandis and G. retisporus, with a distinct zone
without ornamentation near hilar appendix. A preliminary
phylogenetic evaluation, including other available micro
and macromorphological characters, indicates that spore
ornamentation provides significant evolutionary
information within the genus.
782 - The relevance of anamorphic states for a natural
classification of Agaricales: results from morphological
and molecular phylogenetic studies
G. Walther 1* & M. Weiß 2
1 University of Jena, Dornburger Str. 159, D-07743 Jena,
Germany. - 2 University of Tuebingen, Auf der Morgenstelle
1, D-72076 Tuebingen, Germany. - E-mail:
Grit.Walther@rz.uni-jena.de
Single-spore cultures of more than 180 species of
Agaricales were studied morphologically and by molecular
phylogenetic methods. The morphological investigations
revealed anamorphic states in more than 110 species,
which are often specific at the genus or family level.
Phylogenetic analysis of the 5' terminal domain of the
nuclear gene coding for the ribosomal large subunit
resulted in several well-supported groups that are
consistent with anamorph morphology. Our results indicate
that the anamorphic state provides valuable characters for a
natural classification in the Agaricales.
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Book of Abstracts
783 - Molecular variation among species of Verticillium
D. Weber 1* , O. Strunnikova 2 & M. Typas 3
1 Dept. of Botany and Range Science, Brigham Young
University, Provo, Utah 84602, U.S.A. - 2 Research Institute
for Agricultural Microbiology, Podbelsky Shoose 3, St.
Petersburg, Pushkin 8 189620, Russia. - 3 Dept. of Biology,
University of Athens, Panepistemiopolis, Athens TK 15701,
Greece. - E-mail: darrell_weber@byu.edu
The genus Verticillium within fungi imperfecti contains a
heterogeneous group of asexual species. Many of these
species are important plant pathogens or entomopathogens.
The classification is based on morphology of the vegetative
reproductive organs namely conidiophores, conidia and
resting structures. Some analyses have been done on
Verticillium using immunochemical and RFLPs methods.
We analyzed the variation of the nuclear ribosomal internal
transcribed spaces (ITS) of the following species: V. alboatrum,
V. dahliae, V. longisporum, V. lamenicola, V.
fungicola, V. catenulatum, V. tricopus, V. nigrescens, V.
chlamydosporium, V. chlamydosporium, V. theobromeae,
and V. psalliotae. The DNA was duplicated using PCR.
The DNA sequences were determined using a Perkin Elmer
ABI PRISM sequencing apparatus. The relationship of the
different species to each other was determined. A
concensus tree was determined for the isolates of V.
dahliae.
784 - The evolution of cyanobacterial lichens inferred
by phylogenetic analyses of combined mtSSU rDNA
and nLSU rDNA sequence data
E. Wiklund * & M. Wedin
Dept. of Ecology and Environmental Science, Umeå
University, SE-901 87 Umeå, Sweden. - E-mail:
elisabeth.wiklund@eg.umu.se
Fungi associating with cyanobacteria have been found
among the oldest verified fungal fossils of the Lower
Devonian Rhynie chert. The extant cyanobacterial lichen
associations, particularly of the Lecanorales suborder
Peltigerineae, are also often claimed to include the most
primitive extant lichens. It is, however, unclear if the
association with cyanobacteria has arisen once or several
times, if the suborder Peltigerineae as currently
circumscribed is monophyletic, or if it is the sistergroup to
the rest of the Lecanorales. Here, we will present
preliminary results from phylogenetic analyses of two
independent gene loci, including representatives of most
families in Lecanorales suborder Peltigerineae, and several
representatives of other Lecanoromycetes. These analyses
are utilised to test several evolutionary hypotheses
regarding the evolution and phylogeny of cyanobacterial
lichen associations.