Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
771 - Are microsporidia really related to fungi ?
Y. Tanabe 1 , M.M. Watanabe 1 & J. Sugiyama 2*
1 National Institute for Environmental Studies, Onogawa
16-2, Tsukuba, Ibaraki 305-8506, Japan. - 2 The University
of Tokyo, The University Museum; NCIMB Japan, Res.
Center, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033; Tokai
Univ. Campus, Orido 3-20-1, Shimizu-shi, Shizuoka,
Japan. - E-mail: ujsugi@mail.ecc.u-tokyo.ac.jp
The phylogenetic theory of Microsporidia, which are
amitochondrial intracellular parasites, has been challenged
by recent protein sequence-based phylogenies. These
studies indicate a phylogenetic relationship of
Microsporidia and Fungi, and in some analyses
Microsporidia were demonstrated to have evolved from
within fungal lineages. However, most of these studies did
not include sequences from basal fungal phyla
(Chytridiomycota and Zygomycota), and this biased taxon
sampling could result in an erroneous phylogenetic
relationship of Microsporidia and Fungi. To further
investigate the evolutionary origin of Microsporidia, we
performed molecular phylogenetic analyses with newly
determined RPB1 and EF-1α sequences from basal fungi.
Although the phylogenetic position of Microsporidia in the
EF-1α tree still might be artificially misplaced due to the
unusually high rate of sequence divergence of the
microsporidian EF-1α gene, the phylogenies recovered
based on these two protein sequences do not provide strong
evidences for a close relationship between Microsporidia
and Fungi. Moreover, we have identified within EF-1α
genes a characteristic two amino acid deletion which is
conserved in all fungal sequences currently available,
whereas this deletion is absent in microsporidian
sequences. These results argue against the current view of
Microsporidia as highly degenerate fungi, suggesting that it
still remains unresolved whether Microsporidia and Fungi
are sister taxa.
772 - The genus Physalacria in Japan
I. Tanaka
Lead Discovery Research Labaratories, Sankyo Co., LTD.,
33, Miyukigaoka, Tsukuba-Shi Ibaraki 305-0841, Japan. -
E-mail: itanak@tsuku.sankyo.co.jp
The genus Physalacria (Agaricales) forms elliptical to
subconical and hollow pileus on a central stipe.
Approximately 30 species have been recognized as
members of this genus. Berthier (1985) presented a
worldwide monograph of this genus and three taxa were
recognized based on their original descriptions from Japan.
However, their type specimen could not be found
according to Berthier's monograph. During my
investigation on minute basidiomycetes in Japan, several
Physalacria taxa were collected and cultured. The aims of
the present study are to 1) reexamine the type specimens of
the 3 Japanese taxa previously described, 2) examine the
Physalacria flora in Japan and 3) document their cultural
morphology and mating behavior. In the three taxa, only
the type specimen of P. orientaliswas preserved in good
condition and redescribed. The other two taxa were
considered to be doubtful species. In total, 11 taxa
including 6, which were not previously described, were
recognized in Japan. The cultural morphology of 8 taxa out
of 11 were examined and supported the species
delimitation in colony appearance, basidiocarp or stroma
formation, and presence or absence of clamp connections.
Mating behavior was detected for 5 taxa; two taxa
exhibited a bifactorial mating system and the other three
taxa exhibited an amphithallic mating system.
773 - Pairing test among the strains of Trametes
versicolor group from different populations from
Central and South-eastern Europe
M. Tomsovsky * , L. Homolka & F. Nerud
Institute of Microbiology, Academy of Science of Science of
the Czech Republic, Videnska 1083, Prague 4, 142 20,
Czech Republic. - E-mail: tomsovsk@natur.cuni.cz
Mating experiments were carried out to investigate genetic
relationships of different strains of Trametes versicolor
group. Collections of Trametes versicolor (L.: Fr.) Pilat, T.
hirsuta (Fr.) Pilat and T. ochracea (Pers.) Gilb. et
Ryvarden from different localities in the Czech Republic,
Bulgaria and Montenegro (former Yugoslavia) were
investigated. Monokaryotic single spore isolates were
obtained from basidiocarps collected in the field, from
dikaryotic cultures fruited in the laboratory or by bile-salts
dedikaryotization. Monokaryons originating from 30
different dikaryons were mated each other. All pairings
between different species were indicated by a line of
demarcation between the paired isolates. Pairing of
monokaryons belonging to the same species resulted
mostly in the formation of heterokaryotic mycelium and no
line of demarcation was observed in any case. No
intersterility groups were found within the same species.
Experiments confirmed the validity of contemporary
species concept of Trametes versicolor group. This work
has been supported by grant no. 526/02/1216 from the
Grant Agency of the Czech Republic and by Institutional
Research Concept no. AV0Z5020903
774 - Species delimitation in the Russula clelandii
complex from Australia
J.E. Tonkin 1* , T. Lebel 2 , T.W. May 2 & C.B. McLean 1
1 The University of Melbourne, ILFR., Dept Resource
Management, Burnley Campus, Swan Street, Richmond,
3121, Victoria, Australia. - 2 Royal Botanic Gardens
Melbourne, Birdwood Avenue, South Yarra 3141, Victoria,
Australia. - E-mail: Jenny.Tonkin@rbg.vic.gov.au
Book of Abstracts 233