Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme IV: POPULATION DYNAMICS AND ECOLOGY Posters
SSRs regions. The amplified PCR products were cloned,
sequenced and analyzed for SSRs. Primers were designed,
flanking the SSRs found in these amplicons. Seventy-five
isolates from four populations were tested with ten SSR
markers. A high gene diversity was found (h=0,56).
Analysis of the G statistics showed a low degree of
population structure in Central Europe and a high level of
gene flow between populations (Gst=0,02, Nm=17,17). In
contrast, a high population subdivision was found between
the Japanese and the European populations, indicating
strong geographic isolation. These data based on C.
polonica closely resemble those recently produced for its
insect vector, I. typographus (Stauffer et al., Mol. Ecol. 8,
763-773). The SSR primer pairs could also amplify PCR
products from six other Ceratocystis spp. from conifers in
the C. coerulescens complex. Results from this study
suggest that quarantine strategies in Europe should
consider the entire range of genetic diversity on the
continent and that movement of C. polonica and its vectors
between Europe and Japan should be avoided.
1004 - Differentiation of the macrofungal communities
in high diversity forest ecosystems of central Balkan
peninsula
Z. Marjanovic 1* & B. Karadzic 2
1 Eberhard Karls Universität Tuebingen, Physiol. Ökologie
der Pflanzen, Auf der Morgenstelle 1, 72076 Tuebingen,
Germany. - 2 Institut za bioloska istrazivanja 'Sinisa
Stankovic', 29 Novembra 142, 11 000, Beograd,
Yugoslavia. - E-mail: zaklina.marjanovic@unituebingen.de
To study macrofungal communities in forest ecosystems
we chose a canyon in Western Serbia where the vegetation
showed very high biodiversity and differentiation due to
the climatic conditions. Three ecological groups of
macrofungi (wood and litter decayers and mycorrhizal)
were detected by observing sporocarps during 6 years.
Plant and fungal community differentiation were examined
comparing three techniques of multivariate analyses. To
determine the influence of abiotic factors on differentiation
of plant and macrofungal communities we used a
calibration method for humidity, temperature, soil acidity,
available nitrogen and light intensity. We used canonical
correspondence analysis for analysing influence of abiotic
parameters, as well as for analysing influence of dominant
phanerophytae species on differentiation of macrofungal
communities. Four vegetation complexes were described:
xerophyllous forests of black hornbeam, forests of
xerophyllous oaks, mixed mesophyllous forests of black
hornbeam and mesophyllous beech forests. The highest
macrofungal diversity, as well as the best community
structure and differentiation were detected in oak and beech
forests. The main factors that influenced differentiation of
plant communities were temperature, available nitrogen
and light intensity. Abiotic factors showed much lower
influence on differentiation of macrofungal communities.
The main factor for this process was presence of specific
phanerophytae species.
1005 - Fungal associations of mycorrhizal roots of
conifer seedlings in Lithuanian forest nurseries
A. Menkis * , R. Vasiliauskas, A.F.S. Taylor & R.D. Finlay
Department of Forest Mycology & Pathology, Swedish
University of Agricultural Sciences, P.O. Box 7026, SE-750
07 Uppsala, Sweden. - E-mail:
Audrius.Menkis@mykopat.slu.se
Morphological and molecular identification of fungi
associated with mycorrhizal roots was conducted on 60
pine and spruce seedlings in 5 forest nurseries in Lithuania.
The plants were either grown in nursery beds as bare root
seedlings, in plastic trays or in wrapped polyethylene (WP)
containers. Bare root greenhouse seedlings were also
examined. In total, 5364 & 12441 root tips were
investigated for pine and spruce, respectively. Of the pine
roots, 2131 (39%) were mycorrhizal, and 6527 (52%) of
the spruce roots. The cultivation system had a profound
impact on mycorrhizal colonization for both tree species. In
pine, it was most abundant in the nursery bed bare root
system (49%), while in spruce - in the WP containers 72%
of the roots were mycorrhizal. In total 8658 mycorrhizal
roots were morphotyped, and 7 species were found on pine
and 8 on spruce roots. On pine an unidentified ascomycete
was most common (58% of mycorrhizal tips), while on
spruce a basidiomycete Amphinema byssoides colonized
54% of the mycorrhizal tips. Isolation of the fungi into pure
culture was attempted from 8163 mycorrhizal root tips,
yielding 625 isolates, which were divided into 144 groups
according to morphological characteristics of the
mycelium. One representative of each mycelial group was
subjected to sequencing of the ITS region of the rDNA and
43 fungal species have been determined using the NCBI
BLAST. The most common species were Phialocephala
fortinii (29%) and Phialophora finlandia (37%).
1006 - Rhizomorph Foraging Patterns of Armillaria
gallica and A. mellea from the Ozark Mountains of the
central U.S.A.
J.D. Mihail * & J.N. Bruhn
Dept. of Plant Microbiology & Pathology; University of
Missouri, 108 Waters Hall, Columbia, Missouri, 65211,
U.S.A. - E-mail: mihailj@missouri.edu
Our research is driven by the need to understand inter- and
intra-specific interactions of co-occurring Armillaria genets
with respect to oak deline. Foraging patterns of A. gallica
and A. mellea rhizomorphs were measured in three
experimental series: 1) temporal pattern changes measured
in a uniform environment (agar); 2) pattern responses to
woody baits measured in sand; and 3) pattern responses to
the presence of neighboring genets of the same or different
species, measured in sand. Four response metrics were
used: fractal dimension, number of foraging tips, total
rhizomorph length, and total rhizomorph surface area.
Preliminary analysis indicates that rhizomorph foraging by
Book of Abstracts 303