Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
also known as the ophiostomatoid fungi. Ophiostomatoid
fungi are dispersed by bark beetles (Coleoptera:
Scolytidae) and other phloem feeding and wood boring
beetles or by air-borne and rain-splash inoculum. Since
1992 the assemblages of ophiostomatoid fungi associated
with bark beetles on Norway spruce, Picea abies (Ips
typographus, Ips amitinus, Pityogenes chalcographus,
Hylurgops palliatus, Hylurgops glabratus, Dryocoetes
autographus), European larch, Larix decidua (Ips
cembrae), Swiss stone pine, Pinus cembra (Ips amitinus),
Scots pine, Pinus sylvestris and Austrian pine, Pinus nigra
(Tomicus piniperda, Tomicus minor, Ips sexdentatus), elm,
Ulmus spp. (Scolytus spp.) and European beech, Fagus
sylvatica (Taphrorychus bicolor) have been studied in
Austria. The mycobiota of Tetropium spp. (Coleoptera:
Cerambycidae) on spruce and larch was also investigated.
In addition, a small number of ophiostomatoid fungi were
isolated from conifers and hardwoods without signs of
insect infestation. In total, 40 species of ophiostomatoid
fungi were isolated. These included 3 Ceratocystis spp., 3
Ceratocystiopsis spp., 22 species of Ophiostoma, 5
Leptographium spp., 6 Graphium spp. and 1 Pesotum sp.
This ongoing study has greatly improved our knowledge of
the occurrence, hosts and the vectors of ophiostomatoid
fungi in Austria.
707 - Phylogenetic analyses of four taxa of Fusarium,
based on partial sequences of the translation elongation
factor-1 alpha gene
A.K. Knutsen * , M. Torp & A. Holst-Jensen
National Veterinary Institute, Section of Feed and Food
Microbiology, Ullevaalsveien 68, P.O. Box 8156 Dep,
0033 Oslo, Norway. - E-mail: annkristin.knutsen@vetinst.no
Phylogenetic relationships between four Fusarium species
were studied using parts of the nuclear EF-1α-gene as a
phylogenetic marker. Sequences from 12 isolates of F.
poae, 10 isolates of F. sporotrichioides and 12 isolates of
F. langsethiae Torp & Nirenberg ined. yielded 4, 5 and 5
genotypes respectively. In addition we included one isolate
of F. kyushuense. The aligned sequences were subjected to
neighbor-joining, maximum parsimony and maximum
likelihood analyses. The results from the different analyses
were highly concordant. The EF-1α-based phylogenies
support the classification of F. langsethiae as a separate
taxon in the section Sporotrichiella of Fusarium, as the
closest sister taxon to F. sporotrichioides while F.
kyushuense is the sister taxon to F. poae, corresponding
well with the ability of the former taxa to produce T-2 and
HT-2 toxins. In contrast morphological characters indicate
a closer relationship between F. langsethiae and F. poae on
the one hand, and between F. sporotrichioides and F.
kyushuense on the other hand.
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Book of Abstracts
708 - Chlamydospore formation of Entoloma clypeatum
f. hybridum on mycorrhizas and rhizomorphs associated
with Rosa multiflora
H. Kobayashi 1* & A. Yamada 2
1 Ibaraki Prefectural Forestry Research Institute, Nakamachi,
Naka-gun, Ibaraki 311-0122, Japan. - 2 Faculty of
Agriculture, Shinshu University, Minami-minowa-mura,
kamiina-gun, Nagano 399-4588, Japan. - E-mail:
hisakoba@deneb.freemail.ne.jp
Chlamydospores of Entoloma clypeatum f. hybridum were
described on the mycorrhizas and rhizomorphs associated
with Rosa multiflora. Pinkish mycelia were observed
around rhizomorphs and mycorrhizas of E. clypeatum f.
hybridum associated with R. multiflora. Rhizomorphal
connections with fruiting bodies were traced to identify the
colored mycelia. They were thick walled with roughened
surface, ellipsoid with marginal segments, 12-16 x 5-7
µm (including segments), and hyaline to pinkish color.
Hyaline, roughened-surface and swollen cells were
terminally observed in vegetative hyphae with clamp
connections. Surface view was the same both in the
swollen cells and the spores. Two spores arranged in a
chain were also observed. Fragmented clamp connections
were observed on several hyphal tips. Developmental
pattern of chlamydospore seems to be the Nyctalis type.
This is the first report on chlamydospore formation on the
mycorrhizas in entolomatoid fungi.
709 - A putative hybrid or introgressant between
Ophiostoma ulmi and Ophiostoma novo-ulmi from
Austria, Central Europe
H. Konrad * & T. Kirisits
Institute of Forest Entomology, Forest Pathology and
Forest Protection (IFFF), Universität für Bodenkultur
Wien, Hasenauerstrasse 38, A-1190 Vienna, Austria. - Email:
hkonrad@edv1.boku.ac.at
The ascomycete fungi Ophiostoma ulmi and Ophiostoma
novo-ulmi have been responsible for the two destructive
epidemics of Dutch elm disease since the early 20. century.
Although a strong reproductive barrier operates between
these two species, natural hybridization between them has
been reported (Brasier et al., 1998, Mycol. Res. 102, 45-
57). During recent surveys of the Dutch elm disease
pathogens in Austria an unusual Ophiostoma isolate was
obtained from a twig sample of a diseased elm tree. This
isolate has an unique colony morphology neither
resembling that of O. ulmi nor that of O. novo-ulmi, but
similar to that of certain O. ulmi x O. novo-ulmi laboratory
generated hybrids (Kirisits et al., 2001, Forstwiss. Cbl. 120,
231-241). In laboratory crosses with authenticated isolates
of the Dutch elm disease pathogens this strain proved to be
sterile as recipient (female), while it behaved like O. novoulmi
ssp. americana as donor (male) in crosses with both
subspecies of O. novo-ulmi as recipient. The DNA