Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme I: BIODIVERSITY AND CONSERVATION Posters
information from the projects working on specific groups.
Scientific experts will guarantee the high quality standard
of the database. Eventually, scientists will not only be able
to search for specific parasite-host-interactions, also a
literature reference and geographic relations are given.
Major achievements of the GLOPP-LIT project are a
digitization of the major published host-pathogen lists for
Europe. In addition, highly detailed literature reviews of
the distribution of Erysiphales, Peronosporales, and
Uredinales in Germany are currently developed. These will
lead to an improved understanding of the prevalence and
distribution of important pathogen groups. It is hoped that
the tools and processes currently developed for this
purpose can be used to develop similar data sets in other
countries as well.
515 - Identification of basidiomycetes using image
analysis of pigments and colony morphology
M.E. Hansen 1* , P.W. Hansen 2 , S. Landvik 3 , M. Sasa 3 , K.F.
Nielsen 4 & J.M. Carstensen 2
1 IMM, DTU, Richard Petersens Plads, build 321, DK-2800
Lyngby, Denmark. - 2 Videometer, Lyngsoe Alle 3, DK-2970
Hoersholm, Denmark. - 3 NovoZymes A/S, Smoermosevej
25, DK-2880 Bagsværd, Denmark. - 4 BioCentrum-DTU,
Soeltofts Plads, build 221, DK-2800 Lyngby, Denmark. -
E-mail: meh@imm.dtu.dk
It has been shown that image-analysis can be used for the
identification of terverticillate species of Penicillium based
on colour-calibrated images obtained from the
VideometerLab system. The capability of this newly
developed method to distinguish between fungal cultures
that appear identical but are known to be different species
is very convincing and implies its usefulness in recognizing
fungal cultures. The establishment of a public database of
images captured under standardized conditions will allow
any scientist using the system to compare their data to
reference information in the database. The species tested so
far, however, represent only a minor part of the fungal
diversity. Furthermore, these species often produce
strongly pigmented cultures. In this study, we have
challenged the system's ability to recognize and group
species and strains of two genera of the Basidiomycota;
three species each of Polyporus (Polyporales) which
produce whitish/lightly coloured mycelia, and of Pholiota,
Agaricales, which form mottled cultures. A total of 21
isolates were cultivated in triplicates on three different
media in 9 cm Petri dishes, and images were captured of
each plate on day 8 and 15. The results of the imageanalyses,
including groupings of strains and their growth
on the various media were compared to the metabolite
profile and taxonomy of the groups. The system facilitates
comparison of subtle visual differences in an accurate and
reproducible way.
516 - Multispectral macroscopy for mycology
P.W. Hansen * & J.M. Carstensen
Videometer A/S, Lyngsø Allé 3, DK-2970 Hørsholm,
Denmark. - E-mail: PWH@videometer.com
Imaging technology has proved very useful for
classification of fungi which are difficult to separate by
other means without performing a labor demanding
chemical analysis. Studies have been carried out using
traditional trichromatic camera technology, producing three
images corresponding approximately to the colors red,
green, and blue, which are sufficient for many purposes
where information on subtle color differences in the visible
region is required. A new and innovative technology based
on light emitting diodes (LEDs) that adds to the advantages
of the trichromatic technology, is presented. By combining
LEDs with a black-and-white digital camera, multiple
advantages are obtained, of which an increased spatial
resolution (in the megapixel range) and the possibility of
using wavelengths outside the visible range, such as
ultraviolet and near-infrared light, are the most notable. At
present, up to ten relevant wavebands may be combined
into the same unit producing multispectral images
incorporating information not visible to the human eye,
such as information on metabolites or chemical
composition. In order to reduce the wealth of information
produced by a multispectral unit to simple properties
perceivable to the human brain, multivariate statistical
methods are applied to the image data. By supervised or
unsupervised learning these methods are used for building
mathematical models relating image data to properties of
interest, e.g. species, strain, or clone.
517 - Host preferences of wood-decaying
basidiomycetes in a cool-temperate area of Japan
T. Hattori
Forestry and For. Prod. Res. Inst., Norin-Kenkyu-Danchi,
Tsukuba, Ibaraki 305-8687, Japan. - E-mail:
hattori@ffpri.affrc.go.jp
I examined host ranges of wood-decaying basidiomycetes
in a cool-temperate forest in Japan. Fagus spp. and
Quercus spp. are the main tree species within the forest. I
marked fallen trees (more than 20 cm diam and 2 m long)
within a 300 x 200 m plot, then listed polypores, stereoid,
and hydnoid fungi on each tree. Diameter, tree species
were also recorded for each tree. In total, 250 trees were
marked, then 51 species of fungi (44 polypores) were
recorded. Following species did not show any preference:
Bjerkandera adusta, Ganoderma applanatum, Rigidoporus
cinereus, Stereum ostrea, Trametes versicolor, etc. All
collections of Daedalea dickinsii (26/26) were on Quercus
spp. (including Castanea crenata). Other species as follows
were also recurrent on Quercus spp.: Hymenochaete
rubiginosa (39/41; 2 on undetermined trees), Melanoporia
castanea (18/18), Piptoporus soloniensis (8/8), Xylobolus
Book of Abstracts 157