Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
741 - Characterization of Lactarius tesquorum
ectomycorrhizae on Cistus sp. and molecular phylogeny
of related European Lactarius taxa
J. Nuytinck 1 , A. Verbeken 1 , A.C. Rinaldi 2 & O.
Comandini 3*
1 Ghent University, Dpt. Biology, Group Mycology, K.L.
Ledeganckstraat 35, B-9000 Gent, Belgium. - 2 Cattedra di
Chimica Biologica, Università di Cagliari, I-09042
Monserrato Cagliari, Italy. - 3 Dipartimento di Scienze
Ambientali, Università de LAquila, Via Vetoio Loc.
Coppito, I-67100 LAquila, Italy. - E-mail:
comandin@univaq.it
Lactarius is one of the larger genera of ectomycorrhizal
Basidiomycota, with about 400 species recognized
worldwide. Members of the genus have been reported in
association with numerous trees and shrubs, and their
important ecological role as late-stage root colonizers in a
range of ectotrophic plant communities is largely
appreciated [1]. We have started a study aimed at
extending our knowledge of the distribution, phylogeny,
and ectomycorrhizal biology of Lactarius species occurring
in selected ecosystems [2]. In the frame of this research, we
have identified the ectomycorrhizae formed by Lactarius
tesquorum on Cistus sp., one of the most common and
ecologically relevant shrubs present in the semi-arid
regions in the Mediterranean basin, and described them in
terms of morphological, anatomical, and molecular
features. In addition, an ITS rDNA sequence-based
phylogenetic analysis of the related European Lactarius
taxa currently classified together with L. tesquorum,
subgenus Piperites section Piperites, was carried out in
order to clarify species-level relationships. [1] Hutchison,
L.J. (1999) Lactarius. In: Ectomycorrhizal Fungi: Key
Genera in Profile. Cairney, J.W.G. and Chambers, S.M.
(eds.), 269-285. Springer-Verlag, Berlin Heidelberg [2]
Eberhardt, U. et al. (2000) Mycologia 92, 860-873.
742 - A molecular phylogeny of coprophilous fungi of
the Delitschiaceae and Sporormiaceae sensu Barr
(Pleosporales, Ascomycota)
Å. Nyberg * & M. Wedin
Department of Ecology and Environmental Science, Umeå
University, SE-901 87 Umeå, Sweden. - E-mail:
asa.nyberg@eg.umu.se
Preliminary results are presented from an ongoing study of
the phylogeny of Pleosporales utilising nLSU-sequences,
with special focus on the families Delitschiaceae and
Sporormiaceae sensu Barr. The main aims of the study are
to test the circumscription of these families, and to trace the
evolution of coprophilous taxa.
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Book of Abstracts
743 - Karyological and molecular phylogenetic
evaluation on Wallemia sebi
G. Okada * & M. Takashima
Japan Collection of Microorganisms, RIKEN (The Institute
of Physical and Chemical Research), 2-1 Hirosawa, Wako,
Saitama 351-0198, Japan. - E-mail:
okada@jcm.riken.go.jp
Wallemia sebi (Fr.) Arx is a unique osmophilic fungus with
some mysterious aspects. Its phylogenetic relationship and
accurate life cycle have not yet been revealed. It can be
treated as an anamorphic ascomycete
(http://www.ncbi.nlm.nih.gov/; Kirk et al., 2001) or a
teleomorphic basidiomycete (Moore, 1986). Pioneer
researches on nuclear behavior in sporogenesis and septal
pore structure were carried out by Hashmi and Morgan-
Jones (1973) and Terracina (1974), respectively. In this
study, we tried to evaluate W. sebi with the focus on
molecular phylogeny based on nuclear rDNA sequences
and karyology especially in the very unique sporogenesis
using some authentic strains (e.g., JCM 8329, 11230,
11231; http://www.jcm.riken.go.jp/). Based on
nSSU/nLSU rDNA sequence analyses (this study) and
dolipore-like septum (Terracina, 1974; Moore, 1980), it
became clear that W. sebi has basidiomycetous affinities.
Our molecular phylogenetic researches showed that this
fungus presumably locates at the base in the
Ustilaginomycetes as a new clade. Moreover, our
karyological research focusing on the number and size of
nuclei in sporogenesis of W. sebi by using fluorescence
microscopy and nuclear staining method agreed fairly well
with the hypothesis proposed by Moore (1986). As a
conclusion of the moment, we believe that W. sebi is very
possibly a teleomorphic basidiomycete.
744 - A revision of Gymnopilus in New Zealand
A.-M.B. Oliver & D.A. Orlovich *
Department of Botany, University of Otago, PO Box 56,
Dunedin, New Zealand. - E-mail:
david.orlovich@botany.otago.ac.nz
Gymnopilus P. Karsten is a genus of wood-rotting
basidiomycetes with over 150 species distributed
worldwide. Gymnopilus produces a rusty-brown spore print
and has rough spore ornamentation, commonly without a
plage. The taxonomy of Gymnopilus in New Zealand is
revised using numerical analysis. Ten species are recorded
and described, including one new species, six new records
for New Zealand (G. ferruginosus, G. allantopus, G.
tyallus, G. austropicreus, G. norfolkensis and G.
mullaunius) and three that were previously known (G.
junonius, G. crociphyllus and G. mesosporus). This
revision indicates a close relationship between New
Zealand and Australian Gymnopilus and it is suggested that
studies of related genera may reveal similar close
associations of species.