Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
The monospecific genus Hydnodon was described by
Banker (1913), based on Hydnum thelephorum (Léveillé,
1844) because of the salmon-pinkish spore print and shape
and ornamentation of the spores. This genus has been
placed in different families of Aphyllophoralles such as
Hydnaceae (Banker, 1913; Miller, 1933; Harrison, 1973),
Thelephoraceae (Donk, 1964), and Hydnodontaceae
(Jülich, 1981) using different morphological characters.
Hydnodon has also been related with Scytinopogon because
the presence of cyanophilic spores in both genera (Maas
Geesteranus, 1963; Jülich, 1981). This research attempts to
evaluate the phylogenetic affinities of Hydnodon based on
recent phylogenetic hypothesis for the
Homobasidiomycetes (Hibbett, 1997, Hibbett & Thorn,
2001). DNA sequences of the nuc-SSU and mt-SSU of H.
thelephorus were added to a data matrix which included
representatives of each major clade of the
Homobasidiomycetes. The results corroborated a
relationship between Hydnodon and Scytinopogon,
supported by high bootstrap and decay values. This
hypothesis agree with Maas Geestaranus (1963) and Jülich
(1981) proposals, and is congruent with the presence of
inflated and gloeoplerous-like hyphae and a strong
cyanophilic reaction of the spore ornamentation in both
genera. Also, the position of Hydnodon and Scytinopogon
in the general phylogeny of the Homobasidiomycetes
suggests the presence of an additional clade, however, it is
necessary to include more evidence to clarify this problem.
749 - Molecular systematics of Ascochyta spp. infecting
legumes
T.L. Peever * , M. Barve & W.J. Kaiser
Washington State University, Pullman, WA 99164-6430,
U.S.A. - E-mail: tpeever@wsu.edu
Host-specific forms of Ascochyta spp. were sampled from
chickpea (Cicer arietinum), faba bean (Vicia faba), lentil
(Lens culinaris), pea (Pisum sativum) and hairy vetch
(Vicia villosa) from various parts of the world and used to
estimate a phylogeny. Previous results from laboratory
crosses indicated that the Ascochyta spp. infecting faba
bean, lentil and chickpea should be considered distinct
biological species. We tested the hypothesis that these
biological species were also phylogenetic species and
extended the phylogeny to include isolates sampled from
other legumes. We sequenced the nuclear ribosomal
internal transcribed spacer (ITS) and a glyceraldehyde-3phosphate
dehydrogenase gene (G3PD) and estimated a
phylogeny for each dataset. Phylogenies estimated from the
two genomic regions were highly congruent with G3PD
revealing significantly more variation. The G3PD
phylogeny revealed 4 clades, each with high bootstrap
support. Clade 1 contained isolates of A. rabiei from
chickpeas, Clade 2 isolates of Phoma medicaginis and
Mycosphaerella pinodes from pea, Clade 3 isolates of A.
pisi from pea and A. fabae from faba bean, and Clade 4
isolates of A. lentis from lentil and Ascochyta sp. from V.
villosa. These data provide strong support for A. rabiei, A.
fabae and A. lentis as phylogenetic and biological species.
The analysis also revealed that A. lentis is most closely
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Book of Abstracts
related the host specific form on V. villosa and that A.
fabae is most closely related to A. pisi on pea.
750 - The phylogenetic history of Cyttaria, an obligate
parasite of Nothofagus
K.R. Peterson * & D.H. Pfister
Harvard University, 22 Divinity Ave., Cambridge, MA
02138, U.S.A. - E-mail: kpeterson@oeb.harvard.edu
The eleven species of the fungal genus Cyttaria
(Cyttariales, Ascomycota) are obligately parasitic on
several species of the Gondwanan angiosperm Nothofagus
(Nothofagaceae, Hamamelididae). Seven Cyttaria species
are endemic to southern South America (Chile and
Argentina), while the other four are endemic to Australasia
(Australia, Tasmania, and New Zealand). The goals of our
present investigation are to: 1) identify the closest relatives
of Cyttaria; 2) infer the phylogenetic relationships between
Cyttaria species; and 3) infer the phylogenetic relationships
within Cyttaria species. Thus, we have begun to construct
molecular sequence data sets from the following markers:
nuclear ribosomal SSU, LSU, and ITS DNA; mitochondrial
ribosomal SSU and LSU DNA; and the nuclear protein
coding gene RPB2. Preliminary results suggest a close
relationship between Cyttaria and the Helotiales. They also
suggest two major lineages of Cyttaria: one composed
entirely of South American species and another composed
of South American and Australasian species. There also
seems to be significant intraspecific variation within certain
of the more widespread species. Ultimately, we will have a
robust means with which to test the widely accepted
hypothesis that the species of Cyttaria and Nothofagus
have cospeciated. We will also be able to examine the
extent to which vicariance versus dispersal can be invoked
to explain the modern distributions of Cyttaria species and
their hosts.
751 - Diaporthe foeniculacea and its Phomopsis
anamorph in Portugal
A.J.L. Phillips
CREM, Faculdade de Ciências e Tecnologia, Universidade
Nova de Lisboa, Quinta da Torre, 2829-516 Caparica,
Portugal. - E-mail: alp@mail.fct.unl.pt
Niessl described Diaporthe foeniculacea on Foeniculum
officinale from a collection by Moller at Coimbra,
Portugal. Wehmeyer considered it to be a species of
Diaporthopsis and transferred it to Diaporthopsis
angelicae. He did not mention the anamorph. Saccardo
described Phoma foeniculina on F. officinale. Camara
transferred the name to Phomopsis foeniculina based on a
collection from Sacavém, Portugal. Neither Saccardo nor
Camara mentioned the teleomorph. In August 2001, a
fungus with the general characteristics of a Diaporthe sp.
was found on a plant of F. vulgare on the island of