Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme V: CELL BIOLOGY AND PHYSIOLOGY Posters These findings confirm our previous observations of mycorrhizas under heavy metal stress. 1160 - Metabolism in ectomycorrhizal preinfection: Expression of malate synthase and acetylCoA acetyltransferase genes from Laccaria bicolor G.K. Podila 1* , S. Balasubramanian 2 & S.T. Hiremath 3 1 University of Alabama, Dept. of Biological Sciences, Huntsville, AL 35899, U.S.A. - 2 Michigan Tech University, Dept. of Biological Sciences, Houghton, MI 49931, U.S.A. - 3 USDA-NEFES, 359 Main Road, Delaware, OH 43015, U.S.A. - E-mail: podilag@email.uah.edu The ectomycorrhizas are beneficial fungal associations with temperate tree roots. Ectomycorrhizal fungi improve survivability, pathogen resistance, drought tolerance and provide mobilized mineral nutrient in exchange for fixed carbon from the plant. Molecular level analysis suggests that, in response to the perception of the plant root, there is a directional shift in fungal cell physiology as early as four hours into the interaction. During the interaction between Laccaria bicolor and Pinus resinosa, we have observed the upregulation of metabolic genes such as Lb-MS coding for a malate synthase and Lb-AAT that codes for an acetylCoA acetyltransferase. The former, one of the two unique enzymes of the glyoxylate pathway and the latter the terminal thiolase of beta oxidation of fatty acids. The expression of these two genes in conjunction with others (LB-AUT7) suggests that recycling of cell contents and breakdown of reserve food and channeling towards growth occur early in the preinfection stage of the symbiosis. Regulation of Lb-MS and Lb-AAT under interaction conditions and under alternate carbon sources have been studied. Lb-MS has been analyzed at the protein level and gene structure of Lb-AATg has been elucidated. We present our findings on these two metabolic genes and their significance in the symbiosis via the agency of the peroxisome. 1161 - Pre-infection and nutrient starvation expression profiling of interaction related ESTs from the ectomycorrhizal fungus Laccaria bicolor G.K. Podila 1* , M.J. Hymes 2 & J.H. Brand 2 1 University of Alabama, Dept. of Biological Sciences, Huntsville, AL 35899, U.S.A. - 2 Michigan Tech University, Dept. of Biological Sciences, Houghton, MI 49931, U.S.A. - E-mail: podilag@email.uah.edu In this study, a 384 member macroarray was created consisting of 340 ESTs derived from mRNA of Laccaria bicolor during early interaction with Pinus resinosa. The macroarray also contained several positive and negative controls as internal standards and to confirm reproducibility and reduce experiment-to-experiment variability. This array was used to study multiple factors of 352 Book of Abstracts interaction, including expression patterns at various time points of early interaction (
IMC7 Main Congress Theme V: CELL BIOLOGY AND PHYSIOLOGY Posters Tehran, Iran. - 3 Faculty of Medical Sciences, Department of Mycology,Tarbiat Modares University, 14115- 111,Tehran, Iran. - E-mail: mrab@institute.pasteur.ac.ir Aflatoxins are secondary fungal metabolites produced by toxigenic strains of Aspergillus flavus and A. parasiticus. The relationship between aflatoxin synthesis with glutathione S-transferase (GST) activity and microscopic morphology in A. parasiticus grown under different culture conditions has been investigated in order to find out the regulatory mechanism(s) of aflatoxin biosynthesis at cellular level. Aflatoxin formation was inhibited in A. parasiticus grown in the presence of various concentrations(1.56-50%, v/v) of aqueous neem leaf extract with a maximum about >90% at 50% concentration (P
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11-17 August 2002 IMC7 The imc7 Org
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Page 301 and 302: IMC7 Main Congress Theme IV: POPULA
Page 323 and 324: IMC7 Main Congress Theme V: CELL BI
Page 351: IMC7 Main Congress Theme V: CELL BI
Page 367 and 368: Cavernularia: 356 Cenococcum: 500,
Page 369 and 370: Hyalorbilia: 479 Hyalorhinocladiell
Page 371 and 372: Phlebopus: 828 Pholiota: 304, 515 P
Page 373 and 374: Verrucaria: 616 Verruculina: 963 Ve
Page 375 and 376: Order Index Agaricales: 40, 50, 51,
Page 377 and 378: Bogomolova, E.V.: 1078 Bohar, Gy.:
Page 379 and 380: Forlani, G.: 565 Forsby, A.: 842, 8
Page 381 and 382: Juuti, J.T.: 701, 1126 Kabrick, J.M
Page 383 and 384: Morocko, I.: 859 Moser, J.C.: 426,
Page 385 and 386: Sattayaphisut, W.: 1171 Saunders, E
Page 387 and 388: Vukojevic, J.: 363 Vurro, M.: 116 V
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