Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
and secondary metabolite data for classifying and betatubulin
sequencing for cladifying them. Effective
identification keys can then be developed after this
important step. In general classification and cladification
pointed to the same species and species series (natural
series). For example the series Olsonii is unique both
concerning morphology, physiology and profiles of
secondary metabolites and this is in agreement with
cladistic analysis of beta-tubulin sequence data. Image
analysis of colonies and chromatographic data or other
rapid methods combined with chemometrics can also be
used at a later stage once the classification is clear.
Examples of the use of the different methods will be given.
720 - Tuberculina: relatives of rusts parasitize rusts
M. Lutz 1* , R. Bauer 1 & D. Triebel 2
1 Universität Tübingen, Spezielle Botanik/Mykologie, Auf
der Morgenstelle 1, 72072 Tübingen, Germany. -
2 Botanische Staatssammlung München, Menzinger Strasse
67, 80638 München, Germany. - E-mail:
matthias.lutz@uni-tuebingen.de
The morphology, ultrastructure, molecular phylogeny, and
infection biology of some species of the hyphomycetous
genus Tuberculina Sacc. were examined. The presented
results indicate that (1) Tuberculina belongs to the
Basidiomycetes and is closely related to the Uredinales, (2)
Tuberculina species are parasites on the haplontic stage of
their rust hosts and show a distinct mode of interaction, and
that (3) the commonly used species concept within the
genus does not meet all aspects of the phylogeny inferred
from molecular data.
721 - Identification of a basidiomycetous fungus with
high ability to degrade dioxins
N. Maekawa 1* , H. Suhara 2 , R. Kondo 2 & K. Sakai 2
1 The Tottori Mycological Institute, 211, Kokoge, Tottori
689-1125, Japan. - 2 Faculty of Agriculture, Kyushu
University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-
8581, Japan. - E-mail: kin-maek@infosakyu.ne.jp
During screening for efficient biodegraders of various
recalcitrant organopollutants, including dioxins, an
interesting fungus, which possesses a high ability to
degrade toxic dioxins, was isolated from white rot of a
broad-leaved tree from Kyushu Island, Japan. An isolate of
this fungus was inoculated on a sterilized log of Quercus
acutissima. After four months of incubation, it produced a
resupinate and poroid basidioma on the inoculated log. The
basidioma is microscopically characterized by having
monomitic hyphal system, clampless hyphae and clavate
basidia, and by lacking cystidia. These morphological
characteristics and the partial sequencing data of 18S
rDNA indicate that this fungus belongs to the genus
Ceriporia (Basidiomycetes). Among the described species
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Book of Abstracts
of the genus, the fungus is microscopically similar to C.
alachuana and C. ferruginicincta. However, its
basidiospores are broader than those of C. alachuana and
narrower than C. ferruginicincta. Further, the specimens of
C. alachuana and C. ferruginicincta have distinctly poroid
hymenial surface with small angular pores (3-8 and 6-8 per
mm, respectively), whereas hymenial surface of the present
fungus is variable in shape, i.e., poroid with larger pores
(2-6 per mm), lacerate to dentate. These results strongly
indicate that the present fungus is an undescribed species
belonging to the genus Ceriporia.
722 - Phenotypic and molecular variability amongst
isolates of Ceratocystis fimbriata, causing coffee tree
canker in Colombia
M. Marin 1* , B. Castro 2 , A. Gaitan 2 , O. Preisig 1 , B.D.
Wingfield 3 & M.J. Wingfield 1
1 Department of Microbiology and Plant Pathology,
Forestry and Agricultural Biotechnology Institute (FABI),
University of Pretoria, Pretoria, 0002, South Africa. -
2 Centro Nacional de Investigaciones de Café
(CENICAFE), Chinchiná, A.A. 2427, Colombia. -
3 Department of Genetics, Forestry and Agricultural
Biotechnology Institute (FABI), University of Pretoria,
Pretoria, 0002, South Africa. - E-mail:
mauricio.marin@fabi.up.ac.za
One of the most important diseases that reduces the
profitability of coffee plantations in Colombia is coffee tree
canker. This disease, caused by Ceratocystis fimbriata, has
increased in severity and incidence in recent years,
resulting in substantial losses to resource-poor farmers. The
aim of this investigation was to consider the relatedness of
C. fimbriata isolates from different Colombian coffeegrowing
regions, and thus to provide a foundation for
future development of resistant coffee varieties. Fifty
isolates from 11 provinces were characterized based on
morphology and pathogenicity. In addition, isolates were
compared using RAPD and microsatellite markers. DNA
sequence comparsions for the ITS region, Mat-2 HMG Box
and partial β-tubulin gene, were also undertaken. Results of
all comparisions clearly showed that Colombian isolates of
C. fimbriata are highly variable. All molecular parameters
used for these comparisons also showed that C. fimbriata
isolates from Colombia, reside in two major phylogenetic
lineages. We believe that these probably represent distinct
taxa, which we are in the process of characterizing further.
Isolates had different levels of pathogenicity on coffee,
with some causing death of more that 90% and others
killing less than 5% of inoculated plants. There was also a
clear pattern to the distribution of the most pathogenic
strains within coffee growing areas. Our results represent
the first intensive study of this plant pathogen in Colombia.