Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme IV: POPULATION DYNAMICS AND ECOLOGY Posters
the BNR-7 and BP-7 yrs sites, and rapidly increased from
November to January. At the BNR-7 yrs site the number
gradually decreased from 400 in July to 200 in January. At
the BP-7 yrs site, on the other hand, the number gradually
increased from below 100 in July to 200/10 g dry soil in
January. 3. Number of sporangiocarps at the BNR-22 yrs
site was always higher than those at the BNR-7 and BP-7
yrs sites, in the range of 70-110/10 g dry soil, and showed
the higher level in January. At the BNR-7 and BP-7 yrs
sites the number is always very low (below 10/10g dry
soil). 4. At the BNR-7 and BP-7 yrs sites Gigaspora and
Scutellospora types, at the BNR-22 yrs site Glomus and
Sclerocystis types were typically observed.
988 - Seasonal changes in an ectomycorrhizal
community associated with beech (Fagus sylvatica)
R. Kjøller
Department of Mycology, Botanical Institute, University of
Copenhagen, Øster Farimagsgade 2D, DK-1353,
Denmark. - E-mail: rasmusk@bot.ku.dk
The seasonal fluctuations of species abundances in an
ectomycorrhizal (EM) community associated with beech
(Fagus sylvatica) are being studied. Throughout 2001
intensive sampling was performed in a 15 x 15 m plot in
Lille Bøgeskov, located in mid Zealand. Soil cores were
taken at eight harvest times covering the growing season
from March to November. The sampling in this plot will be
continued in 2002-2003. Fungal species forming EM on the
beech roots are sorted by morphotyping and ITS-RFLP and
then identified by sequencing. In the fall 2001,
basidiocarps of EM species were sampled in the plot
(collected and identified by Morten Christensen, KVL,
Denmark). The basidiocarps are being sequenced in order
to create a reference sequence database to aid in the
identification of the root tips. As other EM communities
the beech community turns out to be species rich. So far,
the two harvests from March and May 2001, which has
been most thoroughly worked through, has yielded approx.
40 species. Some of the more abundant species found are
Cortinarius species, Russulaceae spp., chanterelloid spp.
including Clavulina cristata, Cenococcum geophilum,
thelophoroid spp. and Piloderma spp. While some species
e.g. Lactarius subdulcis are present throughout the growing
season others e.g. a yet unidentified chanterelloid sp. are
absent in the spring harvests but are then becoming
dominant in the fall. Possible explanations for the observed
seasonal trends will be discussed.
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Book of Abstracts
989 - Rhizopogon spore bank communities: Species
distribution within and among Californian pine forests
R. Kjøller 1* & T.D. Bruns 2
1 Department of Mycology, Botanical Institute, University
of Copenhagen, Øster Farimagsgade 2D, DK-1353,
Denmark. - 2 Department of Plant and Microbial Biology,
University of California Berkeley, 321C Koshland Hall, CA
94720-3102, U.S.A. - E-mail: rasmusk@bot.ku.dk
In this study we examine the distribution of Rhizopogon
species in spore banks from five Californian pine forests.
Four of the forest sites were discontinuous populations of
Pinus muricata while the last was a Pinus ponderosa stand
in the Sierra National Forest. Rhizopogon species were
isolated from a soil-bioassay using Pinus muricata as host
followed by isolation from individual root tips with typical
Rhizopogon ectomycorrhizal morphology. The cultures
were screened by ITS-RFLP and from each site unique
patterns were sequenced. These sequences were then
compared with sequences derived from identified
sporocarp material. Bioassaying proved to be an efficient
way to bring Rhizopogon species into culture as 50% of the
pots contained ectomycorrhizal tips with Rhizopogon like
morphology and half of these pots then resulted in axenic
Rhizopogon cultures. Members of all the major pineassociated
sections were represented within the isolates.
Our results showed that Rhizopogon spores are
homogeneously distributed within local forest areas while
there is significant structuring at the regional scale. High
spore longevity and homogenization by soil and water
movement may explain their distribution within local forest
areas while the latter observation fits with an expectation of
limited dispersal because of the hypogeous fruiting of
Rhizopogon species.
990 - Monitoring of white-rot fungus during
bioremediation of dioxin-contaminated soils
R. Kondo * , H. Suhara & C. Daikoku
Fac. of Agriculture, Kyushu University, Hakozaki,
Fukuoka, 812-8581, Japan. - E-mail: kondo@brs.kyushuu.ac.jp
Bioremediation is a low-cost treatment alternative for the
cleanup of dioxin-contaminated soils and -fly ashes, when
pollution spread broad-ranging. An interesting fungus was
isolated from white rotten wood of a broadleaf tree from
Kyushu Island in Japan, named strain MZ-340. This isolate
has high ability to degrade dioxin. We have tried to use the
strain MZ-340 for bioremediation of dioxin-contaminated
soil on site. On the other hand, we have to consider that
this trial has potential problems to bring biohazard on
natural ecosystem, when this organism has been
naturalized. Therefore, we have to develop a monitoring
system for the introduced fungus with the examination of
bioremediation. The bioremediation of the dioxincontaminated
soils by the fungus MZ-340 has been