Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
Petr.), and Septoriella panicicola (Petr.) comb. nov. (syn.
Hendersonia panicicola Petr.). Other accepted species
include S. canadensis Nag Raj, S. caroliniana Nag Raj, S.
junci (Desm.) B. Sutton, S. phragmitis Oudem., S. rockiana
(Petr.) Nag Raj and S. thalassica (Speg.) Nag Raj. All other
species names described in Septoriella have been excluded
from the genus and reidentified. This results in 1 new
combination, Phaeoseptaria philippinensis (Sacc.) comb.
nov. (S. philippinensis Sacc.). Septoriella atrata Sacc. is
regarded as a synonym of Septoria heterochora Desm. In
addition, one new species of the genus Linochorella Syd. &
P. Syd., L. cylindrospora sp. nov., is also described.
Related taxonomic problems are also discussed. A key to
all accepted species in the genera Septoriella and
Linochorella is provided.
790 - DNA sequence and IGS RFLP analyses and
molecular markers for identification of Fusarium poae,
F. langsethiae, F. sporotrichioides and F. kyushuense
T. Yli-Mattila 1 , P. Konstantinova 1 , R.L. Mach 2 , I.A.
Alekhina 3* , S.A. Bulat 3 , C.M. Kullnig-Gradinger 2 , C.P.
Kubicek 2 & S.S. Klemsdal 4
1 University of Turku, Lab. of Plant Phys. and Mol. Biol.,
Dept. of Biology, FIN-20014 Turku, Finland. - 2 Technical
University of Vienna, Inst. for Chem. Engineering,
Microbial Biochem., Getreidemarkt 9/166, A-1060 Vienna,
Austria. - 3 Petersburg Nuclear Physics Institute, Lab. of
Eukaryote Genetics, Dept. of Mol. and Radiation
Biophysics, Gatchina 188350, Russia. - 4 The Norwegian
Crop Research Institute, Plant Protection Centre,
Høgskoleveien 7, N-1432 Ås, Norway.
Fusarium langsethiae was recently described to
accommodate 'powdery' isolates of F. poae, which
morphologically resemble F. poae, but whose metabolite
profile (including toxins) is similar to that of F.
sporotrichioides. Sequence analysis of ITS1 and 2
separated F. langsethiae from F. poae, but was unable to
separate all F. sporotrichioides strains from F. langsethiae
strains. Sequence analysis of ß-tubulin distinguished all
four species but did not resolve them phylogenetically. The
high intraspecific variability of the IGS sequences were
found useful to group isolates according to their geographic
origin. Analysis of all combined sequences reliably
separated all species studied. Our results are in accordance
with the results of the UP-PCR hybridization analysis. IGS-
RFLP analysis also allowed differentiation between the
closely related species. According to the phylogenetic
analysis of IGS-RFLP patterns F. langsethiae, F.
sporotrichioides, F. poae and F. kyushuense strains formed
four well-supported clades with high bootstrap values.
Based on the sequence differences in the IGS region,
species-specific primers were designed for the F.
langsethiae/F. sporotrichioides group and for F. poae. The
two phylogenetic subgroups of F. langsethiae, found by
IGS sequencing analysis, were clearly separated on the
basis of size differences of the amplification products with
primers CNL12/PulvIGSr (specific for the F.
langsethiae/F. sporotrichioides group).
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Book of Abstracts
791 - Molecular, morphological and phylogenetic
analysis of Fusarium avenaceum / F. arthrosporioides /
F. tricinctum species complex
T. Yli-Mattila 1* , S. Paavanen-Huhtala 1 , S.A. Bulat 2 , I.A.
Alekhina 2 & H.I. Nirenberg 3
1 University of Turku, Lab. of Plant Phys. and Mol. Biol.,
Dept of Biology, FIN-20014 Turku, Finland. - 2 Petersburg
Nuclear Physics Institute, Lab of Eukaryote Genetics,
Dept. of Mol. and Radiation Biophysics, Gatchina 188350,
Russia. - 3 Inst. fur Mikrobiologie, BBA, Königin-Luise-Str.
19, D-14195 Berlin, Germany. - E-mail: tymat@utu.fi
The great variation in the IGS region offers more potential
to resolve intraspecific phylogenetic relationships, while
beta-tubulin gives more reliable phylogenetic information
at species level. The European F. avenaceum strains of
main group II had identical beta-tubulin sequences with
one American F. avenaceum strain and four European F.
arthrosporioides strains, while F. avenaceum strains of
main group I were closely related to two European F.
arthrosporioides strains and to one Japanese F. anguoides
strain. According to the combined beta-tubulin/IGS/ITS
sequence tree, European F. arthrosporioides strains were
divided into four groups. F. tricinctum strains formed a
well-supported cluster, in which two European clusters
were separated from one African isolate. In the IGS
sequence tree two European F. acuminatum strains
together with one American F. acuminatum strain formed a
cluster, which was separate from another American F.
acuminatum strain. The F. acuminatum cluster was nested
within the large F. tricinctum cluster together with one F.
reticulatum strain in the combined IGS/beta-tubulin tree.
Several strains may be intermediate between the F.
avenaceum / F. arthrosporioides / F. anguoides and F.
tricinctum clusters and represent their own species. These
results are partially supported by the results of UP-PCR
hybridization analysis. Thus the molecular results may be
helpful in future revision in the taxonomy of these species.
792 - Clarification of the taxonomic relationhips among
onion neck rot Botrytis spp.: B. allii is an interspecific
hybrid of B. aclada and B. byssoidea
D.S. Yohalem * , K. Nielsen & M. Nicolaisen
Danish Institute of Agricultural Science, Reserach Center
Flakkebjerg, Slagelse, Denmark. - E-mail:
david.yohalem@agrsci.dk
Three species of Botrytis are recognized as associated with
neck rot of onions. Due to the difficulty of distinguishing
them by morphological criteria and lack of type material
associated with two of the names, several synonomies have
been proposed. Species may be differentiated by conidial
size and shape, but the character is subtle, variable and
there is some overlap. Both the smallest spored group and
the largest spored group have 16 mitotic chromosomes,
while the intermediate group has 32. Based on significant