Book of Abstracts (PDF) - International Mycological Association

IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters
686 - A re-evaluation of Lojkania Rehm, with an
addition of two new species
W.H. Hsieh & C.Y. Chen *
Department of Plant Pathology, National Chung Hsing
University, Taichung, Taiwan. - E-mail:
chiyu86@yahoo.com.tw
The circumscription of Lojkania is amended to merely
include species with germ slits on ascospores. Additional to
the germ slit, there are characters found to be remarkably
correlated and consistent, which coincides with the new
definition of this genus. The ascomatal wall is composed of
characteristically thin sclerotial cells. Smooth ascospores
are 1-septate and uniseriate-arranged in ascus. Asci exhibit
different extent of development in an ascoma, and alike
most bitunicate genera, have no ocular chamber at the tip.
Accordingly, the segregations with its similar genera are,
therefore, readily distinguishable. Two new species are
hitherto erected in Lojkania. They are L. incrassata and L.
wushensis.
687 - Phylogenetic relationships between the genus
Saprolegnia and related genera inferred from ITS
sequences
S. Inaba * & S. Tokumasu
Sugadaira Montane Research Center, University of
Tsukuba, 1278-294 Osa, Sanada-machi, Chiisagata-gun,
Nagano Prefecture, Japan. - E-mail:
s975116@icho.ipe.tsukuba.ac.jp
Phylogenetic relationships among the genera of Isoachlya,
Pythiopsis and Saprolegnia (Saprolegniales, Oomycetes)
were studied based on the sequence data of internal
transcribed spacers including 5.8S ribosomal DNA regions.
The genera share the saprolegnoid type of zoospore
discharge, but they are different in some points. Thus, the
zoospores of Pythiopsis show monoplanetic behavior,
while those of the other genera are diplanetic. Isoachlya is
distinguishable from Saprolegnia by its manner of
zoosporangial renewal. Several species selected from three
genera were sequenced. The data were analyzed with
supplement data of Achlya spp. obtained from GenBank.
The results of the neighbor-joining and the maximum
parsimony analysis showed sequenced species separated
into three clusters. Cluster I consisted of several species of
Isoachlya and Saprolegnia. Cluster II included two species
of Pythiopsis (P. cymosa and P. terrestris) and S.
megasperma. It was discovered that the mucilage outer
layer surrounding young oogonia is a common
characteristic to all of them. Cluster III was composed of
three species with eccentric oospore, i. e. I. eccentrica, P.
humphreyana and S. anisospora. These results suggest that
the orthodox generic classification reflects only the part of
phylogenetic relationships among these organisms. We
reexamine the phylogenetic value of the various
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Book of Abstracts
taxonomical criteria that have been used in the orthodox
classification of the family Saprolegniaceae.
688 - Towards a monograph of the Parmulariaceae
C.A. Inacio * , P.F. Cannon & D.W. Minter
CABI Bioscience, Bakeham Lane, Egham, Surrey TW20
9TY, U.K. - E-mail: c.inacio@cabi.org
The family Parmulariaceae is widespread in the tropics and
contains fungi with a large variety of morphological
characters and host species. 31 genera and more than 100
species are included. Type species of the genera Aldonata,
Aldona, Aulacostroma, Coccodothis, Cocconia,
Cycloschizon, Cyclostomella, Cocconia, Dictyocyclus,
Dothidasteroma, Englerodothis, Ferrarisia,
Hysterostomella, Inocyclus, Kentingia, Kiehlia,
Pachypatella, Palawaniella, Parmularia,
Parmulariopsella, Parmulariopsis, Parmulina,
Perischizon, Polycyclina, Polycyclus, Protothyrium,
Pseudolembosia, Rhagadolobium, Rhipidocarpon,
Symphaeophyma, Thallomyces and their synonyms have
been studied. Illustrations with drawings and
microphotographs have been prepared, and all genera redescribed.
Some characters, including ascospores in a
mucous coat, asci with rostrate dehiscence, centrum
immersed in a gelatinous layer which sometimes becomes
blue in iodine and superficial mycelia with hyphopodiumlike
structures are useful as diagnostic features. Useful
characters for generic delimitation include ascomata
(position in the substrate, shape, outer wall, connection to
the host), superficial mycelium (presence or absence,
position, presence of hyphopodium-like structures) and
ascospores (colour, shape, septa). Comparison will be
made with the possibly related family Asterinaceae and a
new concept for the Parmulariaceae introduced.
689 - Molecular systematics of phylloplane yeasts:
Pigmented species belonging to the Erythrobasidium
lineage of the Urediniomycetes (Basidiomycota)
J. Inácio * , A. Fonseca & I. Spencer-Martins
CREM, SABT, Universidade Nova de Lisboa, Quinta da
Torre, 2829-516 CAPARICA, Portugal. - E-mail:
jjis@mail.fct.unl.pt
A screening of the mycobiota on leaves from selected plant
species (Acer monspessulanum, Quercus faginea, Cistus
albidus, Pistacia lentiscus and Osyris quadripartita),
collected at the 'Arrábida Natural Park', an ecosystem of
Mediterranean characteristics in Portugal, yielded about
830 yeast isolates. Two isolation methods were used: the
conventional method, based on plating of leaf washings
onto solid medium, and the spore-fall method. About 30%
of the isolates, a significant fraction of which producing
ballistoconidia, presented the following characteristics:
production of orange to red pigments, inability to produce