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Book of Abstracts (PDF) - International Mycological Association

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IMC7 Main Congress Theme II: SYSTEMATICS, PHYLOGENY AND EVOLUTION Posters<br />

Madeira. A similar fungus was later found at two separate<br />

localities near Lisbon. Two morphologically distinct<br />

Phomopsis species were found on the stems. The<br />

Diaporthe species were compared with specimens in the<br />

herbaria <strong>of</strong> Padova (PAD) and Coimbra (COI). The<br />

specimen in PAD had unicellular ascospores and appeared<br />

to be immature. A specimen in COI (285) collected by<br />

Moller is a typical Diaporthe and was regarded as D.<br />

foeniculacea. The specimens collected from Madeira and<br />

near Lisbon correlated morphologically with COI 285 and<br />

it is recommended that the name Diaporthe foeniculacea<br />

should be retained. One <strong>of</strong> the Phomopsis spp. on fennel<br />

stems correlated with Camaras description <strong>of</strong> P.<br />

foeniculina, but it is not the anamorph <strong>of</strong> D. foeniculacea.<br />

The teleomorph <strong>of</strong> P. foeniculina has not yet been found.<br />

Diaporthe foeniculacea is self-fertile and readily produces<br />

perithecia in culture.<br />

752 - Characterization <strong>of</strong> Pisolithus in Thailand<br />

C. Phosri 1* , R. Watling 2 , M.P. Martín 3 & A.J.S. Whalley 1<br />

1 School <strong>of</strong> Biomolecular Sciences, Liverpool John Moores<br />

University, Byrom Street, Liverpool, L3 3AF, U.K. -<br />

2 Caledonian <strong>Mycological</strong> Enterprises, Crelah, 26<br />

Blinkbonny Ave, Edinburgh, EH4 3HU, Scotland, U.K. -<br />

3 Real Jardin Botnanico de Madrid, CSIC, Plaza de Murillo<br />

2, 28014, Madrid, Spain. - E-mail: bmscphos@livjm.ac.uk<br />

A detailed study <strong>of</strong> Pisolithus, Scleroderma and Astraeus<br />

from a variety <strong>of</strong> hosts and geographic areas <strong>of</strong> Thailand is<br />

currently being undertaken to assess their potential for<br />

enhancement <strong>of</strong> growth in inoculated seedlings <strong>of</strong> Pinus<br />

kesiya and Eucalyptus camaldulensis. The results<br />

demonstrated that basidiospores from most Pisolithus<br />

isolates are ornamented with well-developed spines. At<br />

least 2 groups <strong>of</strong> spore types occur. Most basidiospores<br />

collected from Eucalyptus are characterized by numerous<br />

echinulate, blunt spines whilst basidiospores with<br />

coalesced spines joined at their tip have been found mainly<br />

in isolates collected from pine forest. This corresponds<br />

with a recent study <strong>of</strong> the internal transcribed spacer (ITS)<br />

DNA sequence. Sequences for 14 Pisolithus isolates<br />

associated with native Mediterranean hosts, Eucalyptus<br />

camaldulensis and Pinus kesiya were compared with those<br />

in GenBank. The phylogenetic analyses grouped Thai<br />

Pisolithus isolates into 2 distinct groups. Group 1 contained<br />

Pisolithus isolates collected from Pine forest and Group 2<br />

contained those collected from Eucalyptus plantation. This<br />

present study indicates that basidiospore ornamentation,<br />

together with ITS sequences, can distinguish two<br />

distinctive Pisolithus groups in Thailand which also exhibit<br />

clear host association.<br />

753 - Cercospora species from Venezuela described by<br />

C. Chupp and A.S. Muller<br />

N. Pons 1* , U. Braun 2 & P.W. Crous 3<br />

1 El Milagro, calle F, número 3, Maracay 2101, Venezuela.<br />

- 2 Martin-Luther-University, Institute <strong>of</strong> Geobotany and<br />

Botanical Garden, Neuwerk 21, D-06099, Halle, Germany.<br />

- 3 University <strong>of</strong> Stellenbosch, Stellenbosch 7602, South<br />

Africa. - E-mail: carinipo@cantv.net<br />

The species Cercospora caracasensis (on Annona<br />

purpurea, Annonaceae), C. centrosematis (on Centrosema<br />

virginianum, Leguminosae-Papilionoideae), C. cestri (on<br />

Cestrum latifolium, Solanaceae), C. chirguensis (on<br />

Curatella americana, Dilleniaceae), C. conocarpi (on<br />

Conocarpus erectus, Combretaceae) and C. cyclantherae<br />

(on Cyclanthera pedata, Cucurbitaceae), described by C.<br />

Chupp and A.S. Muller, were revised. Based on the<br />

morphological characteristics <strong>of</strong> reproductive structures<br />

exhibited by the type specimens and, on the correlation <strong>of</strong><br />

these criteria with available molecular data, the following<br />

new species are proposed: Passalora centrosematis,<br />

Stenella cestri, Pseudocercospora chirguensis and P.<br />

conocarpi. Cercospora caracasensis is reduced to<br />

synonymy with Pseudocercospora annonae-squamosae,<br />

and C. cyclantherae is considered a synonym <strong>of</strong> C. apii<br />

s.lat. Descriptions and illustrations <strong>of</strong> the species treated<br />

are also provided.<br />

754 - Morphological characters <strong>of</strong> Lentinellus cultures<br />

N.V. Psurtseva 1* & Ph.B. Gannibal 2<br />

1 Komarov Botanical Institute RAS, Pr<strong>of</strong>. Popov Str., 2, St.<br />

Petersburg 197376, Russia. - 2 St. Petersburg State<br />

University, Dept. <strong>of</strong> Botany, Universitetskaja nab., 7/9, St.<br />

Petersburg 199034, Russia. - E-mail:<br />

NadyaPsu@NP1512.spb.edu<br />

The taxonomy <strong>of</strong> the genus Lentinellus has recently been<br />

reevaluated by Dr. R.H. Petersen and his Lab (The<br />

University <strong>of</strong> Tennessee, USA) using classical morphology<br />

<strong>of</strong> type specimens, mating study and molecular analysis.<br />

Cultural study <strong>of</strong> Lentinellus strains received from Dr.<br />

Petersen's Lab have been undertaken to evaluate the<br />

diagnostic potential <strong>of</strong> mycelial characters in the genus.<br />

Dikaryon cultures from different intersterility groups<br />

belonging to L. ursinus, L. castoreus, L vulpinus, L.<br />

montanus, L. flabelliformis and L. micheneri were used.<br />

The strains were grown on MEA and PDA media. Indexes<br />

<strong>of</strong> growth, macro- and micromorphology were described<br />

using traditional terminology. Mycelial structure was<br />

studied from scanning electron microscope images.<br />

Laccase and tyrosinase activities were studied by plate<br />

diffusion method (Bavendamm reaction) and spot tests<br />

(substrates: guaiacol, syringaldazine and l-tyrosine). The<br />

strains' capacity for fructification was evaluated on<br />

sawdust/bran substrate. It was shown that different species<br />

were distinguishable by differences in growth indexes,<br />

<strong>Book</strong> <strong>of</strong> <strong>Abstracts</strong> 227

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