Postharvest Biology and Technology of Fruits, Vegetables, and Flowers

PHOSPHOLIPASE D, MEMBRANE DETERIORATION, AND SENESCENCE 215
Table 9.3 Chemical and physical parameters of PLD alpha proteins encoded by genes in tomato (LePLDα1,
LePLDα2, and LePLDα3), strawberry (FaPLDα1), honeydew melon (CmPLDα1 and CmPLDα2), and
cucumber (CsPLDα1)
Amino MM ASP + ARG + Acidic/ Theoretical Grand average
acids (kDa) GLU LYS basic pI hydropathicity
LePLDα1 809 92.2 121 89 A 5.39 −0.447
LePLDα2 807 92.0 112 86 A 5.59 −0.349
LePLDα3 806 92.7 105 94 A − 6.29 −0.378
FaPLDα1 810 91.4 114 91 A 5.76 −0.395
CmPLDα1 808 92.0 122 92 A 5.37 −0.483
CmPLDα2 807 92.1 109 97 A − 6.20 −0.398
CsPLDα1 808 91.8 122 92 A 5.37 −0.472
Parameters shown in columns 1–7 from left to right include total encoded amino acids, molecular mass (MM),
sum of aspartic plus glutamic acid residues (ASP + GLU), sum of arginine plus lysine residues (ARG + LYS),
acidic or basic polypeptide (A, acidic; A − , weakly acidic), theoretical isoelectric point (pI), and grand average
hydropathicity. PLD alpha protein accession numbers are shown in Table 9.2.
phospholipase D alpha 2 (CmPLDa2) [Cucumis melo var. inodorus]
phospholipase D alpha 2 (LePLDa2) [Lycopersicon esculentum]
phospholipase D alpha 3 (LePLDa3) [Lycopersicon esculentum]
phospholipase D1 [Craterostigma plantagineum]
phospholipase D2 [Craterostigma plantagineum]
phospholipase D alpha [Cynara cardunculus]
phospholipase D alpha 1 (PLD alpha 1) [Nicotiana tabacum]
phospholipase D alpha 1 (LePLDa1) [Lycopersicon esculentum]
phospholipase D alpha 1 (PLD alpha 1) [Pimpinella brachycarpa]
phospholipase D alpha [Vitis vinifera]
phospholipase D alpha 2 [Arachis hypogaea]
phospholipase D alpha 1 [Medicago truncatula]
phospholipase D alpha 2 [Medicago truncatula]
phospholipase D alpha 1 (CmPLDa1) [Cucumis melo var. inodorus]
phospholipase D [Cucumis sativus]
phospholipase D alpha [Gossypium hirsutum]
phospholipase D alpha 1 (PLD alpha 1) [Vigna unguiculata]
phospholipase D alpha 2 (PLD 2) [Brassica oleracea]
phospholipase D alpha 1 (PLD 1) [Brassica oleracea]
phospholipase D alpha 1 [Arabidopsis thaliana]
phospholipase D alpha [Arabidopsis thaliana]
phospholipase D Alpha 2 [Arabidopsis thaliana]
phospholipase D alpha 1 precursor (PLD 1) [Ricinus communis]
phospholipase D alpha [Fragaria × ananassa]
phospholipase D2 [Papaver somniferum]
phospholipase D1 [Papaver somniferum]
Fig. 9.11 Phylogenetic tree showing the relatedness of plant PLD alphas from 17 dicot species based on alignment
of their complete deduced amino acid sequences ranging from 806 to 813 residues. Accessions with arrowheads
on the right indicate the seven PLD alpha genes currently being investigated in relation to ripening and senescence
of tomato (Lycopersicon esculentum—light gray), honeydew melon (C. melo—medium gray), and strawberry
(F. × ananassa—dark gray) fruits. The other two highlighted accessions indicate PLD alphas cloned from tissues
of grape berry (V . vinifera—dark gray) and cucumber fruit (C. sativus—light gray).