Postharvest Biology and Technology of Fruits, Vegetables, and Flowers

422 POSTHARVEST BIOLOGY & TECHNOLOGY OF FRUITS, VEGETABLES, & FLOWERS
Resonance signal (RU)
100 200 300 400 500 600
Time (s)
Immobilized
surface
Association
Dissociation
Regeneration
Fig. 20.2
Schematic representation of a Biacore sensorgram.
analysis, whereas the Biacore 3000 is particularly useful for high-sensitivity antibody-based
analyte detection and kinetic analysis. The T100, and recently developed A100, permit highthroughput
analysis of protein–protein and protein–ligand interactions, which may be useful
in the judicious selection of antibodies for specific diagnostic applications. Systems such
as Bio-Rad’s ProteOn XPR36, Nomadic’s SensíQ R○ formats and AlphaSniffer’s common
path interferometry SPR biosensor can also be applied for food quality control, though such
applications are still in development.
An advantage of SPR over other optical methods of detection is that SPR measures the
interaction between immobilized molecules on a surface (e.g., an antigen or an antibody
immobilized onto a sensor chip) and the corresponding ligand in solution passing over this
matrix. This means that the reaction can be measured in a colored solution or in a turbid