Part-I - Controller General of Patents Designs and Trademarks

(12) PATENT APPLICATION PUBLICATION (21) Application No.1317/CHE/2011 A
(19) INDIA
(22) Date of filing of Application :18/04/2011 (43) Publication Date : 21/06/2013
(54) Title of the invention : TECHNIQUES FOR BUFFERLESS LYSING OF CELLS AND SEPARATION OF CELLULAR
COMPONENTS USING MODIFIED MEMBRANES
(51) International classification :C12N
(31) Priority Document No :NA
(32) Priority Date :NA
(33) Name of priority country :NA
(86) International Application No
:NA
Filing Date
:NA
(87) International Publication No : NA
(61) Patent of Addition to Application Number :NA
Filing Date
:NA
(62) Divisional to Application Number
:NA
Filing Date
:NA
(71)Name of Applicant :
1)Indian Institute of Science
Address of Applicant :Indian Institute of Science C.V.
Raman Avenue Bangalore-560012 INDIA Karnataka India
(72)Name of Inventor :
1)Siva Rama Krishna Vanjari
2)Navakanta Bhat
3)SampathSrinivasan
4)Bharadwaj Amrutur
5)Sandeep Keshavan Reddy
6)Deepthi Indukuri
(57) Abstract :
A porous membrane for lysis of a cell population enriched from a biological sample, and isolation of cellular components is
provided. The porous membrane contains embedded lysing agents to perform lysing. The biological sample is brought into contact
with the membrane. Lysis occurs through the action of the embedded lysing agents on the biological sample. The pores of the
porous membrane are designed to have dimensions to allow only a desired type of cellular component(s) resulting from lysis to
pass through the membrane, thereby achieving isolation of the desired cellular component(s). The action of lysing agents is
combined with the filtration properties of porous membranes resulting in an easy-to-use and cost-effective technique.
No. of Pages : 16 No. of Claims : 21
The Patent Office Journal 21/06/2013 13786